Difference between revisions of "OroboPOS"

Revision as of 00:17, 20 May 2015

OroboPOS

Description	The OroboPOS is a polarographic oxygen sensor (POS), with an amperometric mode of operation. The OroboPOS meets the highest quality criteria in terms of linearity, stability and sensitivity of the signal. The Clark type polarographic oxygen sensor (POS) remains the gold standard for measuring dissolved oxygen in biomedical, environmental and industrial applications over a wide dynamic oxygen range. It consitsts of a gold cathode, a silver/silverchloride anode and a KCl electrolyte reservoir separated from the sample by a 25 µm membrane (FEP). The main body of the OroboPOS is made of PEEK. With application of a polarization voltage (0.8 V), a current is obtained as an amperometric signal, which is converted to a voltage.
Product ID	26000-01
Type	O2k, OroboPOS
Link	MiPNet19.18B POS-Service, Gnaiger 1983 POS
Image

Specifications

From nM to mM O₂ - a million-fold dynamic range: The dynamic range of oxygen measurement spans from air saturation (about 200 µM) to the normoxic intracellular range (10-30 µM) and severe hypoxia (<0.1 µM), but also to hyperoxia (oxygen saturation: c. 1 mM). In measurements of cell respiration in conjunction with the Titration-Injection microPump TIP2k, allow accurate steady-state measurement of oxgen levels and respiration with resolution of better than ±1 nM O₂.

Shipment

O2k-Core: 2 OroboPOS are included and shipped in the O2k-Service Box. A spare (third) OroboPOS may be added to the items ordered with the O2k-System.

OroboPOS are protected in a transparent perspex vial screwed onto a blue POM base, with the OroboPOS-Membrane Ring arrested on the POS shaft between the POM base and the thicker part of the POS head which holds an O-ring\Viton\8x1 mm.

POS service and membrane mounting

>> O2k-Manual for OroboPOS Service

>> OroboPOS-Service Kit

POS calibration and SOP

>> MiPNet19.18D O2k-Calibration

>> MiPNet06.03 POS-Calibration-SOP

References

Gnaiger E, Forstner H, eds (1983) Polarographic Oxygen Sensors. Aquatic and Physiological Applications. Springer, Berlin, Heidelberg, New York: 370 pp.
Gnaiger E (2001) Bioenergetics at low oxygen: dependence of respiration and phosphorylation on oxygen and adenosine diphosphate supply. Respir Physiol 128: 277-297.
Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial Dysfunction in Drug-Induced Toxicity (Dykens JA, Will Y, eds) John Wiley: 327-352.

@@ Line 5: / Line 5: @@
 |product id=26000-01
 |product type=O2k, OroboPOS
-|info=[[MiPNet19.18B POS-Service]], [[Gnaiger_1983_POS]]
+|info=[[MiPNet19.18B POS-Service]], [[Gnaiger 1983 POS]]
 |product image=[[Image:OroboPOS sensor head.jpg|right|180px]]
 }}
@@ Line 19: / Line 19: @@
 == POS service and membrane mounting ==
->> [[MiPNet19.18B POS-Service |O2k-Manual for OroboPOS Service]]
+:>> [[MiPNet19.18B POS-Service |O2k-Manual for OroboPOS Service]]
+:>> [[OroboPOS-Service Kit]]
->> [[OroboPOS-Service Kit]]
-== OroboPOS technical support ==
-{{Technical support integrated}}
-If a [[Stirrer test]] shows a slow response of the sensor, see more details in [[Slow responding sensor]].
-As the heart of the OROBOROS O2k the OroboPOS is the prime suspect when technical problems are encountered. However, frequently problems can be traced either to inadequate software settings or to other hardware components, such as the [[POS connector (technical service) |POS connector]]). Therefore, it is important to [[Locating a problem |locate the problem]], primarily with a [[Sensor test]].
-=== OroboPOS technical support pages ===
-{{#ask: mainlabel=Title|[[Category:All]] | [[Technical service::O2 sensor||O2 signal ]]| ?Technical service}}
-=== Problems that require a full Sensor test to locate the problem ===
-* Unstable O2 signal.
-* Unstable O2 flux.
-* High O2 signal at air saturation.
-* High O2 signal at zero oxygen.
-=== Special cases: no further localization necessary ===
-* [[Slow responding sensor]], if observed in a [[Stirrer test]]. Note: The stirrer test is part of the sensor test. It is therefore recommended to do a full [[Sensor test]] whenever problems are encountered.
-=== General trouble shooting procedure for the OroboPOS ===
-Confirm the problem by doing a [[Sensor test]] in the absence of biological material
-==== Case 1.A: No problem was visible in the [[Sensor test]] ====
-Check the following DatLab settings:
-* [[Gain (O2 channel)]]
-* [[POS calibration]]
-* Scaling
-If the problem is not visible when observing the [[Raw signal]] but is visible when observing the calibrated oxygen signal, then there is probably a problem with the [[POS calibration]].
-==== Case 1.B: The problem was visible in the [[Sensor test]] ====
-* Proceed to [[ Locating a problem|Locate the Problem]] and continue as shown below.
-==== Case 2.A: The problem could be localized on the [[OroboPOS]] ====
-'''Solutions''': Follow the instructions for oxygen sensor service [[MiPNet08.04]]. Apply [[contact oil]] to the gold pin and thread connecting the [[OroboPOS]] and [[sensor connector]]. After a sensor service or after applying a new membrane, repeating the [[sensor test]]. In some cases the O2k may be left running over night to achieve a stable signal, as seen by a repeated [[sensor test]].
-If all service precautions fail to solve the problem, the [[OroboPOS]] has to be sent to OROBOROS INSTRUMENTS for further service (a recovery cannot be guaranteed), or a new sensor is applied.
-==== Case 2.B: The problem was not located on the [[POS]] ====
-'''Solutions''': If the problem was localized on an other component follow the instructions for this component. If you could not locate the problem, contact OROBOROS INSTRUMENTS.
+== POS calibration and SOP ==
+:>> [[MiPNet19.18D O2k-Calibration]]
+:>> [[MiPNet06.03 POS-Calibration-SOP]]
 == References ==