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OroboPOS

From Bioblast

                



OroboPOS

O2k-Catalogue

Description The OroboPOS is a polarographic oxygen sensor (POS), with an amperometric mode of operation. With application of a polarization voltage (0.6 to 0.8 V), a current is obtained as an amperometric signal, which is converted to a voltage.

Shipment: OroboPOS are protected in a transparent perspex vial screwed onto a blue POM base, with the OroboPOS-Membrane Ring arrested on the POS shaft between the POM base and the thicker part of the POS head which holds an O-ring\Viton\8x1 mm. The main body of the OroboPOS is made of PEEK.

Product ID 26000-01
Type O2k, OroboPOS
Link Previous Product ID 26100-24, MiPNet08.04, Gnaiger_1983_POS
Image
OroboPOS sensor head.jpg
  • O2k-Core: 2 OroboPOS are included. A spare (third) OROBoPOS may be added to the items ordered with the O2k-System. OroboPOS are shipped with the O2k in the O2k-Service Box.


POS Service and Membrane Mounting

Please refer to the newest version of the manual B MiPNet08.04 POS-Service. If a Stirrer test shows a slow response of the sensor some hints in Slow responding sensor.

Trouble shooting

As the heart of the OROBOROS Oxygraph the Polarographic oxygen sensor is of course the prime suspect when any kind of troubles are encountered. However, frequently problems can be traced either to inadequate software settings or to other hardware components (like the POS connector). Therefore it is important to confirm the problem doing a Sensor test and to localize the problem.

Problems that require a full Sensor test and localization of the problem

  • unstable O2 signal
  • unstable O2 flux
  • high O2 signal at air saturation
  • high O2 signal at zero oxygen

Special cases: no further localization necessary

General Trouble Shooting Procedure for the OroPos

Confirm the problem by doing a Sensor test in the absence of biological material

Option 1.A: No problem was visible in the Sensor test

check the following DatLab settings

If the problem is not visible when observing the Raw signal but is visible when observing the calibrated oxygen signal, then there is probable a problem with the POS calibration.

Option 1.B: The problem was visible in the Sensor test

Option 2.A: The problem could be localized on the POS

Solutions 2.A Follow the instructions for oxygen sensor service [MiPNet08.04]. Use this opportunity to apply contact oil to the gold pin and thread connecting POS and sensor connector. After a sensor service or after applying anew membrane allow the oxygraph to run over night to achieve a stable signal before repeating the sensor test. If all service precautions fail to solve the problem, the POS has to be sent to us for further service (a recovery can not be guaranteed), or a new sensor is to be applied.

Option 2.B: The problem was not localized on the POS

Solutions 2.B If the problem was localized on an other component follow the instructions for this component. If you could not locate the problem contact OROBOROS Instruments.

OroboPOS Technical Service Pages

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... more about "OroboPOS"
The OroboPOS is a polarographic oxyg … The OroboPOS is a polarographic oxygen sensor (POS), with an amperometric mode of operation. With application of a polarization voltage (0.6 to 0.8 V), a current is obtained as an amperometric signal, which is converted to a voltage. </br></br>Shipment: OroboPOS are protected in a transparent perspex vial screwed onto a blue POM base, with the OroboPOS-Membrane Ring arrested on the POS shaft between the POM base and the thicker part of the POS head which holds an O-ring\Viton\8x1 mm. The main body of the OroboPOS is made of PEEK.main body of the OroboPOS is made of PEEK. +
26000-01 +
O2k +  and OroboPOS +
O2 sensor +  and O2 signal +