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OroboPOS

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OroboPOS

O2k-Catalogue

Description The OroboPOS is a polarographic oxygen sensor (POS), with an amperometric mode of operation. The OroboPOS meets the highest quality criteria in terms of linearity, stability and sensitivity of the signal. The Clark type polarographic oxygen sensor (POS) remains the gold standard for measuring dissolved oxygen in biomedical, environmental and industrial applications over a wide dynamic oxygen range.

It consitsts of a gold cathode, a silver/silverchloride anode and a KCl electrolyte reservoir separated from the sample by a 25 µm membrane (FEP). The main body of the OroboPOS is made of PEEK. With application of a polarization voltage (0.8 V), a current is obtained as an amperometric signal, which is converted to a voltage.

Product ID 26000-01
Type O2k, OroboPOS
Link MiPNet19.18B POS-Service, Gnaiger_1983_POS
Image
OroboPOS sensor head.jpg

Specifications

From nM to mM O2 - a million-fold dynamic range: The dynamic range of oxygen measurement spans from air saturation (about 200 µM) to the normoxic intracellular range (10-30 µM) and severe hypoxia (<0.1 µM), but also to hyperoxia (oxygen saturation: c. 1 mM). In measurements of cell respiration in conjunction with the Titration-Injection microPump TIP2k, allow accurate steady-state measurement of oxgen levels and respiration with resolution of better than ±1 nM O2.

Shipment

OroboPOS are protected in a transparent perspex vial screwed onto a blue POM base, with the OroboPOS-Membrane Ring arrested on the POS shaft between the POM base and the thicker part of the POS head which holds an O-ring\Viton\8x1 mm.

POS service and membrane mounting

>> O2k-Manual for OroboPOS Service

>> OroboPOS-Service Kit


OroboPOS technical support


Template NextGen-O2k.jpg


MitoPedia O2k and high-resolution respirometry: O2k-Open Support 



If a Stirrer test shows a slow response of the sensor, see more details in Slow responding sensor.

As the heart of the OROBOROS O2k the OroboPOS is the prime suspect when technical problems are encountered. However, frequently problems can be traced either to inadequate software settings or to other hardware components, such as the POS connector). Therefore, it is important to locate the problem, primarily with a Sensor test.

OroboPOS technical support pages

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Problems that require a full Sensor test to locate the problem

  • Unstable O2 signal.
  • Unstable O2 flux.
  • High O2 signal at air saturation.
  • High O2 signal at zero oxygen.

Special cases: no further localization necessary

General trouble shooting procedure for the OroboPOS

Confirm the problem by doing a Sensor test in the absence of biological material

Case 1.A: No problem was visible in the Sensor test

Check the following DatLab settings:

If the problem is not visible when observing the Raw signal but is visible when observing the calibrated oxygen signal, then there is probably a problem with the POS calibration.

Case 1.B: The problem was visible in the Sensor test

Case 2.A: The problem could be localized on the OroboPOS

Solutions: Follow the instructions for oxygen sensor service MiPNet08.04. Apply contact oil to the gold pin and thread connecting the OroboPOS and sensor connector. After a sensor service or after applying a new membrane, repeating the sensor test. In some cases the O2k may be left running over night to achieve a stable signal, as seen by a repeated sensor test. If all service precautions fail to solve the problem, the OroboPOS has to be sent to OROBOROS INSTRUMENTS for further service (a recovery cannot be guaranteed), or a new sensor is applied.

Case 2.B: The problem was not located on the POS

Solutions: If the problem was localized on an other component follow the instructions for this component. If you could not locate the problem, contact OROBOROS INSTRUMENTS.


References

  • Gnaiger E, Forstner H, eds (1983) Polarographic Oxygen Sensors. Aquatic and Physiological Applications. Springer, Berlin, Heidelberg, New York: 370 pp.
  • Gnaiger E (2001) Bioenergetics at low oxygen: dependence of respiration and phosphorylation on oxygen and adenosine diphosphate supply. Respir Physiol 128: 277-297.
  • Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial Dysfunction in Drug-Induced Toxicity (Dykens JA, Will Y, eds) John Wiley: 327-352.