Difference between revisions of "Oroboros Ecosystem Agenda 2024 Archive"
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== Last week == | == Last week == | ||
2024 Week 06 | |||
* ''' | |||
:::: ''' | * '''Feb-05 Mo''' | ||
:{| | |||
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] | |||
|'''"We demonstrated deficient platelet mitochondrial respiratory chain functions, oxidative phosphorylation (OXPHOS), and electron transfer (ET) capacity with complex I (CI)-linked substrates, and reduced [...] endogenous platelet coenzyme Q10 (CoQ10) concentration in UC [urothelial carcinoma] patients."''' <br> | |||
* Palacka P, Gvozdjáková A, Rausová Z, Kucharská J, Slopovský J, Obertová J, Furka D, Furka S, Singh KK, Sumbalová Z (2021) Platelet mitochondrial bioenergetics reprogramming in patients with urothelial carcinoma. [[Palacka 2021 Int J Mol Sci|»Bioblast link«]] | |||
:from the O2k-Network »[[SK Bratislava Sumbalova Z]]« | |||
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* ''' | * '''Feb-06 Tu''' | ||
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* ''' | * '''Feb-07 We''' | ||
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* '''Feb- | * '''Feb-08 Th''' | ||
:{| | :{| | ||
| width="110" align="center" | [[Image:O2k- | | width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]] | ||
|''' | |'''Can I use the same stock solutions for all reagents for different O2k-chamber volumes?'''<br> | ||
For some reagents, the concentration of the stock solution differs between the 2.0-mL and the 0.5-mL chamber (O2k-sV-Module) to facilitate a precise volume titration.<br> | |||
- »'''[[O2k- | -»'''[[MiPNet09.12 O2k-Titrations]]'''«<br> | ||
- communicated by »[[Timon-Gomez Alba]]« and »[[Leo Elettra]]« | |||
|} | |} | ||
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* '''Feb- | * '''Feb-09 Fr''' | ||
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* '''Feb-10 Sa''' | |||
[[File:Year of the Dragon.jpg|400px]] [[File:ChineseNewYear2024 Taiwan.jpg|400px]]<br> | |||
== Jan 2024 == | == Jan 2024 == | ||
2024 Week 01 | 2024 Week 01 | ||
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* '''Jan-26 Fr''' | * '''Jan-26 Fr''' | ||
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2024 Week 05 | |||
* '''Jan-29 Mo''' | |||
:::: '''»[[E-mit Winter School 2024 Brixen IT]]«''' Jan 28 - Feb 02 | |||
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* '''Jan-30 Tu''' | |||
:::: '''16:30 [[Gnaiger Erich|Erich Gnaiger]] presenting at [[E-mit Winter School 2024 Brixen IT]]''' - ''Coupling control and pathway control in mitochondrial high-resolution respirometry'' | |||
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* '''Jan-31 We''' | |||
:{| | |||
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]] | |||
|''' Can the S-pathway O<sub><small>2</small></sub> flux be derived by subtracting N-pathway O<sub><small>2</small></sub> flux from NS-pathway O<sub><small>2</small></sub> flux?'''<br> | |||
No, the sum of the O<sub><small>2</small></sub> fluxes measured in the presence of NADH-linked substrates alone and succinate as the single substrate is typically higher than using a combination of all these substrates. This effect is described as incomplete additivity: <br> | |||
- »'''[[Additive effect of convergent electron flow]]'''« <br> | |||
- communicated by »[[Cardoso Luiza]]« and »[[Grings Mateus]]« | |||
|} | |||
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* '''Feb-01 Th''' | |||
:{| | |||
| width="110" align="center" | [[Image:O2k-Feedback.jpg|60px|link=https://wiki.oroboros.at/index.php/O2k-Feedback |O2k-Feedback]] | |||
|''' ''“Doing the entire hands-on module from start to finish was the best for really “cementing” theory and hands-on application together.”'''''<br> | |||
[[Cosgrove Richard]] from Eli Lilly Research Labs, on what he liked best on [[IOC162]]<br> | |||
- »'''[[O2k-Feedback]]'''«<br> | |||
|} | |||
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* '''Feb-02 Fr''' | |||
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Revision as of 09:46, 12 February 2024
Oroboros Ecosystem Agenda 2024 Archive
- Mitochondria and Cell Research - the Oroboros Ecosystem
Last week
2024 Week 06
- Feb-05 Mo
"We demonstrated deficient platelet mitochondrial respiratory chain functions, oxidative phosphorylation (OXPHOS), and electron transfer (ET) capacity with complex I (CI)-linked substrates, and reduced [...] endogenous platelet coenzyme Q10 (CoQ10) concentration in UC [urothelial carcinoma] patients."
- Palacka P, Gvozdjáková A, Rausová Z, Kucharská J, Slopovský J, Obertová J, Furka D, Furka S, Singh KK, Sumbalová Z (2021) Platelet mitochondrial bioenergetics reprogramming in patients with urothelial carcinoma. »Bioblast link«
- from the O2k-Network »SK Bratislava Sumbalova Z«
- Feb-06 Tu
- Feb-07 We
- Feb-08 Th
Can I use the same stock solutions for all reagents for different O2k-chamber volumes?
For some reagents, the concentration of the stock solution differs between the 2.0-mL and the 0.5-mL chamber (O2k-sV-Module) to facilitate a precise volume titration.
-»MiPNet09.12 O2k-Titrations«
- communicated by »Timon-Gomez Alba« and »Leo Elettra«
- Feb-09 Fr
- Feb-10 Sa
Jan 2024
2024 Week 01
- Jan-01 Mo
- Jan-02 Tu
New year, new state? What is REN state?
REN state is now distinguished from ROX state: REN is measured in mitochondrial preparations without addition of substrates, whereas ROX is due to oxidative side reactions remaining after inhibition of the electron transfer pathway.
- »REN«, »ROX«
- communicated by »Timon-Gomez Alba« and »Leo Elettra«
- Jan-03 We
- Jan-04 Th
"Oxygen Consumption Rate (OCR) measurements of fresh and cryopreserved placental specimens are comparable whereas snap frozen procedure impairs mitochondrial activity"
- Giovarelli M, Serati A, Zecchini S, Guelfi F, Clementi E, Mandò C (2023) Cryopreserved placental biopsies maintain mitochondrial activity for high-resolution respirometry. »Bioblast link«
- from the O2k-Network »IT Milan Clementi E«
- Jan-05 Fr
2024 Week 02
- Jan-08 Mo
Do you need to export DatLab traces for your publication?
Save your graph as an editable picture using "Copy to clipboard" in DatLab 7.4 (.wmf) or "Save" in DatLab 8 (.svg).
- »Copy to clipboard in DatLab 7«, »Download DatLab 8«
- communicated by »Baglivo Eleonora« and »Grings Mateus«
- Jan-09 Tu
- Jan-10 We
- »AICBC 2024 Navi Mumbai IN« Jan 10-12
- Jan-11 Th
What are special features of the new SmartPOS (O2k-Series J and NextGen-O2k-Series XB)?
- "The electrical connections of the SmartPOS are protected thus there is no need to clean them."
- "The serial number of the SmartPOS is automatically recognized by the DatLab 8 software."
»MiPNet28.10 SmartPOS-service«
- communicated by »Grings Mateus« and »Baglivo Eleonora«
- Jan-12 Fr
2024 Week 03
- Jan-15 Mo
Why electron transfer pathway and not electron transfer chain?
The term “chain” suggests a linear sequence, however, the convergence of electron flow at the Q-junction indicates a higher degree of complexity, making “pathway” a more fitting descriptor.
- »Electron transfer pathway«, »Gnaiger 2020 BEC MitoPathways«
- communicated by »Leo Elettra« and »Timon-Gomez Alba«
- Jan-16 Tu
- Jan-17 We
- Jan-18 Th
The study on rats by Jedlička et al indicates that "...the concept of general pattern of age-dependent decline in mitochondrial oxygen consumption across different organs and tissues could be misleading."
- Jedlička J, Tůma Z, Razak K, Kunc R, Kala A, Proskauer Pena S, Lerchner T, Ježek K, Kuncová J (2022) Impact of aging on mitochondrial respiration in various organs. »Bioblast link«
- from the O2k-Network »CZ_Pilsen_Kuncova_J«
- Jan-19 Fr
2024 Week 04
- Jan-22 Mo
“We are truly glad to have your continued support and partnership.”
Lu Amelia from the O2k-Network Lab »TW Taoyuan Sunpoint«
- »O2k-Feedback«
- Jan-23 Tu
- Jan-24 We
- Jan-25 Th
How do I view and change the settings of a measurement/file in DatLab 8?
"O2k control (active) will always show the settings of the active recording [and allow changes], while O2k info (view) will show the settings of the recorded selected file."
-»MiPNet28.07 NextGen-O2k Series XB manual«; »MiPNet28.08 Oroboros O2k Series J manual«
- communicated by »Baglivo Eleonora« and »Grings Mateus«
- Jan-26 Fr
2024 Week 05
- Jan-29 Mo
- »E-mit Winter School 2024 Brixen IT« Jan 28 - Feb 02
- Jan-30 Tu
- 16:30 Erich Gnaiger presenting at E-mit Winter School 2024 Brixen IT - Coupling control and pathway control in mitochondrial high-resolution respirometry
- Jan-31 We
Can the S-pathway O2 flux be derived by subtracting N-pathway O2 flux from NS-pathway O2 flux?
No, the sum of the O2 fluxes measured in the presence of NADH-linked substrates alone and succinate as the single substrate is typically higher than using a combination of all these substrates. This effect is described as incomplete additivity:
- »Additive effect of convergent electron flow«
- communicated by »Cardoso Luiza« and »Grings Mateus«
- Feb-01 Th
“Doing the entire hands-on module from start to finish was the best for really “cementing” theory and hands-on application together.”
Cosgrove Richard from Eli Lilly Research Labs, on what he liked best on IOC162
- »O2k-Feedback«
- Feb-02 Fr