Palmitoyl-CoA: Difference between revisions

Latest revision as of 17:08, 18 January 2022

high-resolution terminology - matching measurements at high-resolution

Palmitoyl-CoA

Description

Palmitoyl-CoA is a coenzyme A derivative of palmitate formed by acyl-CoA synthase. In contrast to medium- and short-chain acyl-CoA, palmitoyl-CoA cannot freely diffuse into the mitochondrial matrix. Formation of palmitoylcarnitine by CPTI is necessary prior to transfer into mitochondria for further fatty acid oxidation (β-oxidation). To study Fatty acid oxidation using Palmitoyl-CoA, Carnitine and low amount of malate is needed on mitochondrial preparations.

Application in HRR

Pa-CoA: Palmitoyl-CoA (Palmitoyl-Coenzyme A lithium salt; C₃₇H₆₅N₇O₁₇P₃S · xLi⁺), Sigma-Aldrich: P9716, store at -20 °C, CAS: 188174-64-3, M = 1004.94 g·mol^-1

Preparation of 10 mM stock solution (dissolved in H₂O):

Weigh 5 mg Palmitoyl-CoA.
Add 497.5 µL H₂O.
Divide into 100 µL portions.
Store at -20°C.

»O2k manual titrations MiPNet09.12 O2k-Titrations

Titration volume: 8 µL 10 mM using a 25 µL Hamilton syringe (2 mL O2k-Chamber).
Final concentration: 40 µM.
Comments: Please consider that the final concentration needed in the O2k-Chamber may vary according to your cell type and overtitration of Palmitoyl-CoA might inhibit the respiration.

MitoPedia topics: Substrate and metabolite

Labels:

MitoPedia:FAT4BRAIN

@@ Line 1: / Line 1: @@
 {{MitoPedia
-|abbr=
+|description='''Palmitoyl-CoA''' is a coenzyme A derivative of palmitate formed by acyl-CoA synthase. In contrast to medium- and short-chain acyl-CoA, palmitoyl-CoA cannot freely diffuse into the mitochondrial matrix. Formation of palmitoylcarnitine by CPTI is necessary prior to transfer into mitochondria for further fatty acid oxidation (β-oxidation). To study [[Fatty acid oxidation]] using Palmitoyl-CoA, [[Carnitine]] and low amount of malate is needed on mitochondrial preparations.
-|description=PalmitoylCoA is a coenzyme A derivative of palmitate formed by acylCoA synthetase. In contrast to medium- and short-chain acylCoA, palmitoylCoA cannot briefly diffuse into mitochondrial matrix. Formation of palmitoylcarnitine by CPTI is necessary prior transfer into mitochondria for further β-oxidation.
 |type=
 }}
-{{MitoPedia methods
+__TOC__
-|mitopedia method=
-|type=
+== Application in [[HRR]] ==
+{{Chemical_description
+|abbr=Pa-CoA
+|trivial name=Palmitoyl-CoA
+|complete name=Palmitoyl-Coenzyme A lithium salt
+|chem formula=C<sub>37</sub>H<sub>65</sub>N<sub>7</sub>O<sub>17</sub>P<sub>3</sub>S · xLi<sup>+</sup>
+|molar mass=1004.94
+|vendor=Sigma-Aldrich
+|product number=P9716
+|store at=-20 °C
+|sensitivity=
+|cas=188174-64-3
+|h statements=
+|h info=
+}}<!--:::'''Pa-CoA: Palmitoyl-CoA''' (Palmitoyl-Coenzyme A lithium salt; Sigma Aldrich, P9716, 5 mg, store at -20°C; M = 1004.94 g·mol<sup>-1</sup>-->
+::::'''Preparation of 10 mM stock solution''' (dissolved in H<sub>2</sub>O):
+::::# Weigh 5 mg Palmitoyl-CoA.
+::::# Add 497.5 µL H<sub>2</sub>O.
+::::# Divide into 100 µL portions.
+::::# Store at -20°C.
+:::»'''O2k manual titrations''' [[MiPNet09.12 O2k-Titrations]]
+::::* Titration volume: 8 µL 10 mM using a 25 µL Hamilton syringe (2 mL O2k-Chamber).
+::::* Final concentration: 40 µM.
+::::* Comments: Please consider that the final concentration needed in the O2k-Chamber may vary according to your cell type and overtitration of Palmitoyl-CoA might inhibit the respiration.
+{{MitoPedia methods|type=
 }}
 {{MitoPedia topics
-|mitopedia topic=
+|mitopedia topic=Substrate and metabolite
 |type=
+}}
+{{Labeling
+|additional=MitoPedia:FAT4BRAIN
 }}