Paumelle 2019 J Hepatol

[[Has title::Paumelle R, Haas J, Hennuyer N, Bauge E, Deleye Y, Mesotten D, Langouche L, Vanhoutte J, Cudejko C, Wouters K, Hannou SA, Legry V, Lancel S, Lalloyer F, Polizzi A, Smati S, Gourdy P, Vallez E, Bouchaert E, Derudas B, Dehondt H, Gheeraert C, Fleury S, Tailleux A, Montagner A, Wahli W, Van Den Berghe G, Guillou H, Dombrowicz D, Staels B (2019) Hepatic PPARα is critical in the metabolic adaptation to sepsis. J Hepatol 70:963-73.]]

» [[Has info::PMID: 30677458]]

Was written by::Paumelle R, Was written by::Haas J, Was written by::Hennuyer N, Was written by::Bauge E, Was written by::Deleye Y, Was written by::Mesotten D, Was written by::Langouche L, Was written by::Vanhoutte J, Was written by::Cudejko C, Was written by::Wouters K, Was written by::Hannou SA, Was written by::Legry V, Was written by::Lancel S, Was written by::Lalloyer F, Was written by::Polizzi A, Was written by::Smati S, Was written by::Gourdy P, Was written by::Vallez E, Was written by::Bouchaert E, Was written by::Derudas B, Was written by::Dehondt H, Was written by::Gheeraert C, Was written by::Fleury S, Was written by::Tailleux A, Was written by::Montagner A, Was written by::Wahli W, Was written by::Van Den Berghe G, Was written by::Guillou H, Was written by::Dombrowicz D, Was written by::Staels B (Was published in year::2019) Was published in journal::J Hepatol

Abstract: [[has abstract::Although the role of inflammation to combat infection is known, the contribution of metabolic changes in response to sepsis is poorly understood. Sepsis induces the release of lipid mediators, many of which activate nuclear receptors such as the peroxisome proliferator-activated receptor (PPAR)α, which controls both lipid metabolism and inflammation. However, the role of hepatic PPARα in the response to sepsis is unknown.

Sepsis was induced by intraperitoneal injection of Escherichia coli in different models of cell-specific Pparα-deficiency and their controls. The systemic and hepatic metabolic response was analysed using biochemical, transcriptomic and functional assays. PPARα expression was analysed in livers from elective surgery and critically ill patients and correlated with hepatic gene expression and blood parameters RESULTS: Both whole body and non-hematopoietic Pparα-deficiency in mice decreased survival upon bacterial infection. Livers of septic Pparα-deficient mice displayed an impaired metabolic shift from glucose to lipid utilization resulting in more severe hypoglycemia, impaired induction of hyperketonemia and increased steatosis due to lower expression of genes involved in fatty acid catabolism and ketogenesis. Hepatocyte-specific deletion of PPARα impaired the metabolic response to sepsis and was sufficient to decrease survival upon bacterial infection. Hepatic PPARA expression was lower in critically ill patients and correlated positively with expression of lipid metabolism genes, but not with systemic inflammatory markers.

Metabolic control by PPARα in hepatocytes plays a key role in the host defense to infection. Lay summary: As the main cause of death of critically ill patients, sepsis remains a major health issue lacking efficacious therapies. While current clinical literature suggests an important role for inflammation, metabolic aspects of sepsis have been mostly overlooked. Here, we show that mice with an impaired metabolic response, due to deficiency of the nuclear receptor PPARα in the liver, exhibit enhanced mortality upon bacterial infection despite a similar inflammatory response, suggesting that metabolic interventions may be a viable strategy for improving sepsis outcomes.

Copyright © 2019. Published by Elsevier B.V.]] • Keywords: has publicationkeywords::Hepatocytes, has publicationkeywords::Inflammation, has publicationkeywords::Metabolism, has publicationkeywords::Nuclear receptors, has publicationkeywords::Sepsis • Bioblast editor: [[has editor::Plangger M]] • O2k-Network Lab: Was published by MiPNetLab::FR Lille Duez H, Was published by MiPNetLab::FR Lille Lancel Steve

Labels: MiParea: MiP area::Respiration  Pathology: Diseases::Infectious, Diseases::Sepsis 

Organism: Organism::Human  Tissue;cell: tissue and cell::Liver  Preparation: Preparation::Homogenate 

Coupling state: Coupling states::LEAK, Coupling states::OXPHOS  Pathway: Pathways::F, Pathways::N  HRR: Instrument and method::Oxygraph-2k