Pyruvate: Difference between revisions
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|abbr=P | |abbr=P | ||
|description=[[File:Pyruvic_acid.jpg|left|80px|Pyruvic acid]] | |description=[[File:Pyruvic_acid.jpg|left|80px|Pyruvic acid]] | ||
'''Pyruvic acid''', C<sub>3</sub>H<sub>4</sub>O<sub>3</sub>, is an alpha-keto acid which occurs under physiological conditions mainly as the anion '''pyruvate<sup>-</sup>, P''', with ''p''K<sub>a</sub> = 2.5. Pyruvate is formed in glycolysis from phosphoenolpyruvate. In the cytosol, pyruvate is a substrate of [[lactate dehydrogenase]]. Pyruvate enters the mitochondrial matrix via a specific low ''K''<sub>m</sub>' | '''Pyruvic acid''', C<sub>3</sub>H<sub>4</sub>O<sub>3</sub>, is an alpha-keto acid which occurs under physiological conditions mainly as the anion '''pyruvate<sup>-</sup>, P''', with ''p''K<sub>a</sub> = 2.5. Pyruvate is formed in glycolysis from phosphoenolpyruvate. In the cytosol, pyruvate is a substrate of [[lactate dehydrogenase]]. Pyruvate enters the mitochondrial matrix via a specific low ''K''<sub>m</sub>' H<sup>+</sup>/monocarboxylate cotransporter known as the [[pyruvate carrier]]. In the mt-matrix the oxidative decarboxylation of pyruvate is catalyzed by [[pyruvate dehydrogenase]] and yields [[acetyl-CoA]]. Pyruvate competitively reverses the inhibition of [[Complex IV | cytochrome ''c'' oxidase]] by [[cyanide]]. Pyruvate is an antioxidant reacting with [[hydrogen peroxide]]. | ||
In the mt-matrix the oxidative decarboxylation of pyruvate is catalyzed by [[pyruvate dehydrogenase]] and yields [[acetyl-CoA]]. | |info=[[Gnaiger 2014 MitoPathways]], [[MiPNet09.12]] | ||
Pyruvate competitively reverses the inhibition of [[Complex IV | cytochrome ''c'' oxidase]] by cyanide. Pyruvate is an antioxidant reacting with [[hydrogen peroxide]]. | |||
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|type=Substrate ETS | |type=Substrate ETS | ||
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: Prepare fresh everyday. | : Prepare fresh everyday. | ||
::1 | ::1. Weigh 44 mg of pyruvic acid directyl into a 1 ml Eppendorf tube. | ||
::2 | ::2. Add 0.2 ml H<sub>2</sub>O. | ||
::3 | ::3. Adjust pH to 7.0. | ||
Revision as of 01:00, 10 March 2015
Description
Pyruvic acid, C3H4O3, is an alpha-keto acid which occurs under physiological conditions mainly as the anion pyruvate-, P, with pKa = 2.5. Pyruvate is formed in glycolysis from phosphoenolpyruvate. In the cytosol, pyruvate is a substrate of lactate dehydrogenase. Pyruvate enters the mitochondrial matrix via a specific low Km' H+/monocarboxylate cotransporter known as the pyruvate carrier. In the mt-matrix the oxidative decarboxylation of pyruvate is catalyzed by pyruvate dehydrogenase and yields acetyl-CoA. Pyruvate competitively reverses the inhibition of cytochrome c oxidase by cyanide. Pyruvate is an antioxidant reacting with hydrogen peroxide.
Abbreviation: P
Reference: Gnaiger 2014 MitoPathways, MiPNet09.12
MitoPedia topics: Substrate and metabolite
Application in HRR
P: Pyruvate (pyruvic acid, sodium salt, C3H3O3Na); Sigma P 2256, 25 g, store at 4 ยฐC; FW = 110.0
Preparation of 2 M stock solution (dissolved in H2O)
- Prepare fresh everyday.
- 1. Weigh 44 mg of pyruvic acid directyl into a 1 ml Eppendorf tube.
- 2. Add 0.2 ml H2O.
- 3. Adjust pH to 7.0.
Oxygraph-2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume: 5 ยตl using a 25 ยตl syringe (2 ml O2k-chamber).
- Final concentration: 5 mM.