Difference between revisions of "Rodrigues 2021 Sci Rep"
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|title=Rodrigues AQ, Picolo VL, Goulart JT, Silva IMG, Ribeiro RB, Aguiar BA, Ferreira YB, Oliveira DM, Lucci CM, de Bem AF, Paulini F (2021) Metabolic activity in cryopreserved and grafted ovarian tissue using high-resolution respirometry. Sci Rep 11:21517. | |title=Rodrigues AQ, Picolo VL, Goulart JT, Silva IMG, Ribeiro RB, Aguiar BA, Ferreira YB, Oliveira DM, Lucci CM, de Bem AF, Paulini F (2021) Metabolic activity in cryopreserved and grafted ovarian tissue using high-resolution respirometry. Sci Rep 11:21517. | ||
|info=[https://www.ncbi.nlm.nih.gov/pubmed/34728762 PMID: 34728762 Open Access] | |info=[https://www.ncbi.nlm.nih.gov/pubmed/34728762 PMID: 34728762 Open Access] | ||
|authors=Rodrigues Aline Q, Picolo Victor L, Goulart Jair T, Silva Isabella M G, Ribeiro Rayane B, Aguiar Beatriz A, Ferreira Yasmin B, Oliveira Daniela M, Lucci Carolina M, de Bem Andreza | |authors=Rodrigues Aline Q, Picolo Victor L, Goulart Jair T, Silva Isabella M G, Ribeiro Rayane B, Aguiar Beatriz A, Ferreira Yasmin B, Oliveira Daniela M, Lucci Carolina M, de Bem Andreza Fabro, Paulini Fernanda | ||
|year=2021 | |year=2021 | ||
|journal=Sci Rep | |journal=Sci Rep | ||
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{{Labeling | {{Labeling | ||
|area=Respiration | |area=Respiration | ||
|injuries=Cryopreservation | |||
|organism=Mouse | |||
|tissues=Genital | |||
|preparations=Homogenate | |||
|couplingstates=LEAK, OXPHOS, ET | |||
|pathways=S | |||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
|additional=2021-11 | |additional=2021-11 | ||
}} | }} |
Revision as of 17:20, 9 November 2021
Rodrigues AQ, Picolo VL, Goulart JT, Silva IMG, Ribeiro RB, Aguiar BA, Ferreira YB, Oliveira DM, Lucci CM, de Bem AF, Paulini F (2021) Metabolic activity in cryopreserved and grafted ovarian tissue using high-resolution respirometry. Sci Rep 11:21517. |
Rodrigues Aline Q, Picolo Victor L, Goulart Jair T, Silva Isabella M G, Ribeiro Rayane B, Aguiar Beatriz A, Ferreira Yasmin B, Oliveira Daniela M, Lucci Carolina M, de Bem Andreza Fabro, Paulini Fernanda (2021) Sci Rep
Abstract: Cryopreservation of ovarian tissue followed by transplantation represents a strategy to restore ovarian function and fertility. Stress from cryopreservation-thawing processes can lead to alterations and/or damage to mitochondrial structure and functionality. High resolution respirometry and histological analysis were used to evaluate the effect of cryopreservation and transplantation on ovarian tissue. Four different conditions were performed: Fresh non-transplanted tissue, Fresh transplanted tissue, Cryopreserved non-transplanted tissue and Cryopreserved transplanted tissue. All groups were able to respond to the substrates-uncoupler-inhibitor protocol. We found a dramatic decrease in general oxygen consumption in hemi-ovaries submitted to cryopreservation and/or transplantation. The effect of cryopreservation on mitochondrial metabolism was less intense than effect of transplantation, since the transplantation affected all of the mitochondrial states. A total of 2644 follicles were analyzed. Of these, 2198 were classified as morphologically normal. The percentage of morphologically normal follicles was significantly lower in the Cryopreserved transplanted group when compared to the Cryopreserved non-transplanted group and the Fresh transplanted group (p-value < 0.05). Despite decreased follicular viability and mitochondrial activity, the cryopreservation followed by transplantation of ovarian tissue proved feasible for attempts to restore ovarian function.
β’ Bioblast editor: Plangger M
Labels: MiParea: Respiration
Stress:Cryopreservation Organism: Mouse Tissue;cell: Genital Preparation: Homogenate
Coupling state: LEAK, OXPHOS, ET
Pathway: S
HRR: Oxygraph-2k
2021-11