- high-resolution terminology - matching measurements at high-resolution
SUIT-003 Ce1;ce2SD;ce3Omy;ce4U-
Description
Abbreviation: CCP(SD)
Reference: A Stadlmann 2006 Cell Biochem Biophys
MitoPedia concepts:
SUIT protocol,
SUIT A
- SUIT-catg: CCP(S)
- SUIT protocol pattern: complex coupling control protocol (CCP)
Coupling control protocol
Step | Respiratory state | Pathway control | Comment |
---|---|---|---|
1R | ROUTINE | ce | pce without substrate are in a state of ROX. |
2S,2D | R+SP | ROUTINE (R) + S (SP) | pce are in state SP. |
3Omy | L+SL | LEAK (L) + S (SL) | ce and pce are in the LEAK state. |
4U | E+SE | ETS (E) + S (SE) | ce and pce are in the ETS state. |
5Rot | ROX+SE | ROX+S (SE) | ce are in the ROX state, pce are in the ETS state. |
6Ama | ROXAma | ROX |
- 1R 1S .. versus 1R 2Omy 3U 4S .. and respiration medium
- Intac cells & succinate: ADP is not permeable through the plasma membrane of intact cells, and this is the case for succinate in many cell types (Steinlechner-Maran 1997 Transplantation). If succinate and ADP are accessible to mitochondria in a fraction of permeabilized (non-viable) cells, respiration will be stimulated, if these mitochondria retain a functional S-pathway (CII-linked respiration). Preservation of mitochondrial respiratory function provides the argument of using mitochondrial respiration medium (MiR05) in this CCP with cells of limited viability (Stadlmann_2006_Cell Biochem Biophys).