Difference between revisions of "SUIT-003 O2 ce D009"
From Bioblast
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::: '''[[SUIT protocol pattern]]:''' linear [[coupling control protocol]] (CCP) | ::: '''[[SUIT protocol pattern]]:''' linear [[coupling control protocol]] (CCP) | ||
::: '''[[Mark names in DatLab]]:''' CCP_02 | ::: '''[[Mark names in DatLab]]:''' CCP_02 | ||
::: '''[[DatLab- | ::: '''[[DatLab-Analysis templates |DatLab-Analysis template]]:''' [[Media:SUIT MiPNet08.09 CellRespiration.xlsx|CCP_02.xlsx]] | ||
[[File:CCP_02(R,L,E).jpg|left|150px]] | [[File:CCP_02(R,L,E).jpg|left|150px]] | ||
:::* The CCP diagram may show the respiratory states directly (left) as an alternative to presenting the titration steps. | :::* The CCP diagram may show the respiratory states directly (left) as an alternative to presenting the titration steps. | ||
Revision as of 16:14, 20 September 2016
- high-resolution terminology - matching measurements at high-resolution
SUIT-003 O2 ce D009
Description
Abbreviation: CCP_R,1Omy,2U-
Reference: A MiPNet08.09 CellRespiration, Huetter 2004 Biochem J
MitoPedia concepts:
SUIT protocol,
SUIT A
- SUIT-catg: CCP
- SUIT protocol pattern: linear coupling control protocol (CCP)
- Mark names in DatLab: CCP_02
- DatLab-Analysis template: CCP_02.xlsx
- The CCP diagram may show the respiratory states directly (left) as an alternative to presenting the titration steps.
- Rot and Ama
- In this CCP, ROX is measured after titration of rotenone, antimycin A, or both in a single step. Validity of using rotenone only for inducing ROX in intact cells is evaluated by stepwise measurement of ROX after titration of rotenone (3Rot) followed by titration of animycin A (4Ama).
CCP_02-Rot,Ama
- CCP_R,1Omy,2U,3Rot-4Ama
- Rot and Ama
- In this CCP, ROX is measured after stepwise titration of rotenone (3Rot) and antimycin A (4Ama). In many intact cells ROX measured after titration of rotenone (3Rot) is not or only slightly further inhibited by subsequent titration of animycin A (4Ama).
- Respiration in senescent human primary fibroblasts (0.2×106 cells/ml). Arrows show steps in the titration regime of the coupling control protocol, inducing the following respiratory states: ROUTINE (R; routine state in cell-culture medium); LEAK (L; inhibition of ATPsynthase by 1 μg/ml oligomycin); ETS (E; maximal stimulation by uncoupling of oxidative phosphorylation in four subsequent titrations of the uncoupler, U, FCCP (2.5–4 μM final concentration); ROX (rotenone, Rot, and antimycin A; residual oxygen consumption). Modified after Huetter_2004_Biochem J.
- Hütter E, Renner K, Pfister G, Stöckl P, Jansen-Dürr P, Gnaiger E (2004) Senescence-associated changes in respiration and oxidative phosphorylation in primary human fibroblasts. Biochem J 380:919-28. - »Bioblast link«
