Difference between revisions of "SUIT-003 O2 ce D028"
From Bioblast
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|abbr=CCP_R,1Omy,2U-3Rot,4S,5Ama | |abbr=CCP_R,1Omy,2U-3Rot,4S,5Ama | ||
|description=[[File:CCP(S)04.jpg|200px]] | |description=[[File:CCP(S)04.jpg|200px]] | ||
|info=CCP_RP2 | |info='''A''' CCP_RP2 | ||
}} | }} | ||
{{MitoPedia concepts | {{MitoPedia concepts |
Revision as of 05:13, 18 September 2016
Description
Abbreviation: CCP_R,1Omy,2U-3Rot,4S,5Ama
Reference: A CCP_RP2
MitoPedia concepts:
SUIT protocol,
SUIT A
- SUIT-catg: CCP(S)
- SUIT protocol pattern: linear coupling control protocol (CCP)
- Mark names in DatLab: CCP(S)R2
- DatLab-Excel template: in prep.: CCP(S)R2.xlsx
R,L,E format
In the diagrams of coupling control protocols for intact cells (CCP), the steps may be replaced by the respiratory states.
- 3Rot,4S-5Ama
- 3Rot: In many intact cells ROX measured after titration of rotenone (3Rot) is not or only slightly further inhibited by subsequent titration of animycin A (CCP_02a.
- 4S: Succinate is not permeable through the plasma membrane of many intact cells (Steinlechner-Maran 1997 Transplantation). If succinate is accessible to rotenone-inhibited uncoupled mitochondria, respiration will be stimulated.
- 5Ama: Inhibition of CIII by antimycin A inhibits succinate-stimulated respiration (Stadlmann_2006_Cell Biochem Biophys).