Difference between revisions of "SUIT-003 O2 ce D028"
From Bioblast
Line 10: | Line 10: | ||
::: '''[[SUIT protocol pattern]]:''' linear [[coupling control protocol]] (CCP) | ::: '''[[SUIT protocol pattern]]:''' linear [[coupling control protocol]] (CCP) | ||
::: '''[[Mark names in DatLab]]:''' CCP(S)R2 | ::: '''[[Mark names in DatLab]]:''' CCP(S)R2 | ||
::: '''[[DatLab- | ::: '''[[DatLab-Analysis templates |DatLab-Analysis template]]:''' ''in prep.'': CCP(S)R2.xlsx | ||
== R,L,E format == | == R,L,E format == |
Revision as of 16:18, 20 September 2016
Description
Abbreviation: CCP_R,1Omy,2U-3Rot,4S,5Ama
Reference: A CCP_RP2
MitoPedia concepts:
SUIT protocol,
SUIT A
- SUIT-catg: CCP(S)
- SUIT protocol pattern: linear coupling control protocol (CCP)
- Mark names in DatLab: CCP(S)R2
- DatLab-Analysis template: in prep.: CCP(S)R2.xlsx
R,L,E format
In the diagrams of coupling control protocols for intact cells (CCP), the steps may be replaced by the respiratory states.
- 3Rot,4S-5Ama
- 3Rot: In many intact cells ROX measured after titration of rotenone (3Rot) is not or only slightly further inhibited by subsequent titration of animycin A (CCP_02a.
- 4S: Succinate is not permeable through the plasma membrane of many intact cells (Steinlechner-Maran 1997 Transplantation). If succinate is accessible to rotenone-inhibited uncoupled mitochondria, respiration will be stimulated.
- 5Ama: Inhibition of CIII by antimycin A inhibits succinate-stimulated respiration (Stadlmann_2006_Cell Biochem Biophys).