SUIT-003 O2 ce D028: Difference between revisions
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m (Gnaiger Erich moved page 1ce;2Omy;3U;4Rot;5S;6Ama to 1ce;2ceOmy;3ceU;4ceRot;5ceS;6ceAma) |
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|abbr=RLE(S) | |abbr=RLE(S) | ||
|description=[[File:CCP(S)04.jpg|200px]] | |description=[[File:CCP(S)04.jpg|200px]] | ||
|info='''A''' CCP(S) | |info='''A''' CCP(S) | ||
}} | }} | ||
{{MitoPedia concepts | {{MitoPedia concepts | ||
|mitopedia concept=SUIT protocol, SUIT A | |mitopedia concept=SUIT protocol, SUIT A | ||
}} | }} | ||
::: '''[[Categories of SUIT protocols |SUIT-category]]:''' | ::: '''[[Categories of SUIT protocols |SUIT-category]]:''' ceRLE(S) | ||
::: '''[[SUIT protocol pattern]]:''' linear [[coupling control protocol]] (CCP) | ::: '''[[SUIT protocol pattern]]:''' linear [[coupling control protocol]] (CCP) | ||
== | == ceR,L,E format == | ||
[[File:CCP(S)04(R,L,E).jpg|200px]] In the diagrams of coupling control protocols for intact cells (CCP), the steps may be replaced by the respiratory states. | [[File:CCP(S)04(R,L,E).jpg|200px]] In the diagrams of coupling control protocols for intact cells (CCP), the steps may be replaced by the respiratory states. | ||
:::* ''' | :::* '''3ceRot,4ceS;5ceAma''' | ||
:::: ''' | :::: '''3ceRot''': In many intact cells ROX measured after titration of rotenone (3ceRot) is not or only slightly further inhibited by subsequent titration of antimycin A. | ||
:::: ''' | :::: '''4ceS''': Succinate is not permeable through the plasma membrane of many intact cells ([[Steinlechner-Maran 1997 Transplantation]]). If succinate is accessible to rotenone-inhibited uncoupled mitochondria in dead cells (dce), respiration will be stimulated. | ||
:::: ''' | :::: '''5ceAma''': Inhibition of CIII by antimycin A inhibits succinate-stimulated respiration ([[Stadlmann_2006_Cell Biochem Biophys]]). |
Revision as of 23:33, 4 August 2017
Description
Abbreviation: RLE(S)
Reference: A CCP(S)
MitoPedia concepts:
SUIT protocol,
SUIT A
- SUIT-category: ceRLE(S)
- SUIT protocol pattern: linear coupling control protocol (CCP)
ceR,L,E format
In the diagrams of coupling control protocols for intact cells (CCP), the steps may be replaced by the respiratory states.
- 3ceRot,4ceS;5ceAma
- 3ceRot: In many intact cells ROX measured after titration of rotenone (3ceRot) is not or only slightly further inhibited by subsequent titration of antimycin A.
- 4ceS: Succinate is not permeable through the plasma membrane of many intact cells (Steinlechner-Maran 1997 Transplantation). If succinate is accessible to rotenone-inhibited uncoupled mitochondria in dead cells (dce), respiration will be stimulated.
- 5ceAma: Inhibition of CIII by antimycin A inhibits succinate-stimulated respiration (Stadlmann_2006_Cell Biochem Biophys).