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Difference between revisions of "SUITbrowser"

From Bioblast
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::::* [[Coupling control state|Coupling control]]
::::* [[Coupling control state|Coupling control]]


::::* [[Oxidative phosphorylation|Physiological pathway control]]
::::* [[Oxidative phosphorylation|OXPHOS Respiration]]
ย 
::::* [[ROUTINE|ROUTINE Respiration]]
ย 
::::* [[NS-pathway_control_state#SUITbrowser_question:_Maximum_physiological_capacity|Maximum physiological capacity]]
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::::* [[Living cells|Cell viability test]]
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::::* [[Crabtree effect]]
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::::* [[AOX|AOX activity]]
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::::* [[MitoKit-CII MitoPedia|Permeable succinate/malonate]]


::::* [[Carbohydrate]]
::::* [[Carbohydrate]]
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::::* [[Glutamate anaplerotic pathway control state|Glutamine/glutamate]]
::::* [[Glutamate anaplerotic pathway control state|Glutamine/glutamate]]
::::* [[Complex_I-linked_substrate_state#SUITbrowser_question:_CI_threshold|CI threshold]]
::::* [[NS-pathway_control_state#SUITbrowser_question:_Maximum_physiological_capacity|Physiological capacity]]


::::* [[Succinate|Succinate pathway]]
::::* [[Succinate|Succinate pathway]]
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::::* [[Q-junction|Q-junction (FNSGp)]]
::::* [[Q-junction|Q-junction (FNSGp)]]


::::* [[Complex_IV#SUITbrowser_question:_CIV|Complex IV (CIV)]]
::::* [[Complex_I-linked_substrate_state#SUITbrowser_question:_CI_threshold|CI threshold]]
ย 
::::* [[Complex_IV#SUITbrowser_question:_CIV|Complex IV activity]]


::::* [[Cytochrome_c#SUITbrowser_question:_mt_outer_membrane_integrity|mt outer membrane integrity]]
::::* [[Cytochrome_c#SUITbrowser_question:_mt_outer_membrane_integrity|mt outer membrane integrity]]


::::* [[Living cells|Cell viability test]]
::::* [[ATPases|Unspecific ATPase activity]]
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::::* [[Digitonin|Optimum digitonin concentration]]
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::::* [[Magnesium Green|Mitochondrial ATP production]]
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::::* [[Mitochondrial membrane potential]]
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::::* [[Hydrogen peroxide|H<sub>2</sub>O<sub>2</sub> - membrane potential dependence]]
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::::* [[Hydrogen peroxide|H<sub>2</sub>O<sub>2</sub> - [O<sub>2</sub>] dependence]]
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::::* [[Hydrogen peroxide|H<sub>2</sub>O<sub>2</sub> - RET initiated]]
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::::* [[Hydrogen peroxide|H<sub>2</sub>O<sub>2</sub> - NADH-pathway initiated]]
ย 


::::* [[Crabtree effect]]


{{MitoPedia concepts
{{MitoPedia concepts

Revision as of 09:06, 2 July 2019


high-resolution terminology - matching measurements at high-resolution


SUITbrowser

Description

Use the SUITbrowser to find the best substrate-uncoupler-inhibitor-titration (SUIT) protocol for your research questions.

Open the SUITbrowser: http://suitbrowser.oroboros.at/

Each SUIT protocol receives a score from 0 to 3 in different categories that correspond to a research question (e.g. information on coupling control, information on fatty acid oxidation, among others). This score is set depending of the information which one can get from each SUIT protocol (3: this SUIT protocol is ideal, highly informative for the specific question; 2: information provided; 1: information provided, but is not a SUIT protocol mainly focused for the study of this question; 0: the specific information/question is not provided by the SUIT protocol). It is possible to choose multiple reasearch questions at a time, to find the best protocols that adress this combination of problems.


Template NextGen-O2k.jpg

Define your experimental strategy

Mitochondrial preparations or living cells

  1. Mitochondrial preparations: imt, thom, pce, pti (including pfi)
  2. Living cells: ce = vce + dce

Applications

  1. O2
  2. H2O2 and O2
  3. Mitochondrial ATP production and O2
  4. Membrane potential and O2


Research questions

The following research questions can be selected in the SUITbrowser in various combinations to provide links to the best SUIT protocols:




MitoPedia concepts: SUIT concept, SUIT protocol 


MitoPedia methods: Respirometry, Fluorometry 


MitoPedia O2k and high-resolution respirometry: DatLab