Difference between revisions of "SUITbrowser"
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::::* [[Coupling control state|Coupling control]] | ::::* [[Coupling control state|Coupling control]] | ||
::::* [[Oxidative phosphorylation| | ::::* [[Oxidative phosphorylation|OXPHOS Respiration]] | ||
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::::* [[ROUTINE|ROUTINE Respiration]] | |||
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::::* [[NS-pathway_control_state#SUITbrowser_question:_Maximum_physiological_capacity|Maximum physiological capacity]] | |||
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::::* [[Living cells|Cell viability test]] | |||
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::::* [[Crabtree effect]] | |||
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::::* [[AOX|AOX activity]] | |||
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::::* [[MitoKit-CII MitoPedia|Permeable succinate/malonate]] | |||
::::* [[Carbohydrate]] | ::::* [[Carbohydrate]] | ||
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::::* [[Glutamate anaplerotic pathway control state|Glutamine/glutamate]] | ::::* [[Glutamate anaplerotic pathway control state|Glutamine/glutamate]] | ||
::::* [[Succinate|Succinate pathway]] | ::::* [[Succinate|Succinate pathway]] | ||
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::::* [[Q-junction|Q-junction (FNSGp)]] | ::::* [[Q-junction|Q-junction (FNSGp)]] | ||
::::* [[Complex_IV#SUITbrowser_question:_CIV|Complex IV | ::::* [[Complex_I-linked_substrate_state#SUITbrowser_question:_CI_threshold|CI threshold]] | ||
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::::* [[Complex_IV#SUITbrowser_question:_CIV|Complex IV activity]] | |||
::::* [[Cytochrome_c#SUITbrowser_question:_mt_outer_membrane_integrity|mt outer membrane integrity]] | ::::* [[Cytochrome_c#SUITbrowser_question:_mt_outer_membrane_integrity|mt outer membrane integrity]] | ||
::::* [[ | ::::* [[ATPases|Unspecific ATPase activity]] | ||
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::::* [[Digitonin|Optimum digitonin concentration]] | |||
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::::* [[Magnesium Green|Mitochondrial ATP production]] | |||
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::::* [[Mitochondrial membrane potential]] | |||
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::::* [[Hydrogen peroxide|H<sub>2</sub>O<sub>2</sub> - membrane potential dependence]] | |||
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::::* [[Hydrogen peroxide|H<sub>2</sub>O<sub>2</sub> - [O<sub>2</sub>] dependence]] | |||
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::::* [[Hydrogen peroxide|H<sub>2</sub>O<sub>2</sub> - RET initiated]] | |||
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::::* [[Hydrogen peroxide|H<sub>2</sub>O<sub>2</sub> - NADH-pathway initiated]] | |||
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{{MitoPedia concepts | {{MitoPedia concepts |
Revision as of 09:06, 2 July 2019
Description
Use the SUITbrowser to find the best substrate-uncoupler-inhibitor-titration (SUIT) protocol for your research questions.
Open the SUITbrowser: http://suitbrowser.oroboros.at/
Each SUIT protocol receives a score from 0 to 3 in different categories that correspond to a research question (e.g. information on coupling control, information on fatty acid oxidation, among others). This score is set depending of the information which one can get from each SUIT protocol (3: this SUIT protocol is ideal, highly informative for the specific question; 2: information provided; 1: information provided, but is not a SUIT protocol mainly focused for the study of this question; 0: the specific information/question is not provided by the SUIT protocol). It is possible to choose multiple reasearch questions at a time, to find the best protocols that adress this combination of problems.
Define your experimental strategy
Mitochondrial preparations or living cells
- Mitochondrial preparations: imt, thom, pce, pti (including pfi)
- Living cells: ce = vce + dce
Applications
- O2
- H2O2 and O2
- Mitochondrial ATP production and O2
- Membrane potential and O2
Research questions
- The following research questions can be selected in the SUITbrowser in various combinations to provide links to the best SUIT protocols:
MitoPedia concepts:
SUIT concept,
SUIT protocol
MitoPedia methods:
Respirometry,
Fluorometry
MitoPedia O2k and high-resolution respirometry:
DatLab