- high-resolution terminology - matching measurements at high-resolution
Safranin
Description
Safranin is amongst the extrinsic fluorophores used in fluorometry and is one of the most established dyes for measuring mitochondrial membrane potential. The excitation wavelength for this dye is 495 nm and the emission wavelength is 587 nm.
Abbreviation: Saf
Reference: Krumschnabel 2014 Methods Enzymol, Kiss 2014 FASEB J, Pham 2014 Am J Physiol, Komary 2010 Biochim Biophys Acta, Figueira 2012 Methods Mol Biol, Kauppinen RA, Hassinen IE (1984) Monitoring of mitochondrial membrane potential in isolated perfused rat heart. Am J Physiol 247: H508-H516.
MitoPedia methods:
Fluorometry
Synonyms
Safranin O, Safranin Y, Saranin T, Gossypimine, Cotton Red, Basic Red2
Application in high-resolution respirometry
Safranin is a potent inhibitor of Complex I linked respiration and of the phosphorylation system. At a concentration of 2 µM, safranin can be applied for simultaneous monitoring of oxygen consumption and mtMP of mt-preparations in a CII linked substrate state.[1] Oroboros Instruments uses Safranin O from Sigma (#S2255, 25 g).
See Discussion.
Data acquisition and analysis
A step-by-step protocol of our current way of acquiring and analyzing Safranin data is given in MiPNet19.19
References
- ↑ Krumschnabel G, Eigentler A, Fasching M, Gnaiger E (2014) Use of safranin for the assessment of mitochondrial membrane potential by high-resolution respirometry and fluorometry. Methods Enzymol 542: 163-181. »Pubmed 24862266
