Saponin: Difference between revisions
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{{MitoPedia | {{MitoPedia | ||
|abbr=Sap | |||
|description='''Saponin''' is a mild detergent that [[Permeabilization of plasma membrane|permeabilizes plasma membranes]] completely and selectively due to their high cholesterol content, whereas mt-membranes with lower cholesterol content are affected only at higher concentrations. Applied for [[Permeabilized tissue or cells|permeabilization of muscle fibres]]. | |description='''Saponin''' is a mild detergent that [[Permeabilization of plasma membrane|permeabilizes plasma membranes]] completely and selectively due to their high cholesterol content, whereas mt-membranes with lower cholesterol content are affected only at higher concentrations. Applied for [[Permeabilized tissue or cells|permeabilization of muscle fibres]]. | ||
|type=Chemicals | |type=Chemicals | ||
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|type=Chemicals | |type=Chemicals | ||
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== Application in [[HRR]] == | |||
'''Sap: Saponin''' (from quillaja bark); Sigma S 2149, 25 g, store at R.T.; FW = 1229.31 | |||
<span style="color:#8B008B"> '''Caution:''' Prepare fresh everyday!</span> | |||
'''Comment:''' ''The product is withdrawn from Sigma!'' | |||
'''Preparation of 5 mg/ml stock solution (dissolved in H<sub>2</sub>O)''': | |||
::1) weigh 5 mg of Saponin | |||
::2) dissolve in 1 ml H<sub>2</sub>O | |||
→ use 50 µg Saponin/ml [[Biopsy preservation solution|BIOPS]]: add 20 µl of 5 mg/ml saponin stock solution per 2 ml BIOPS | |||
Revision as of 11:49, 18 May 2012
- high-resolution terminology - matching measurements at high-resolution
Saponin
Description
Saponin is a mild detergent that permeabilizes plasma membranes completely and selectively due to their high cholesterol content, whereas mt-membranes with lower cholesterol content are affected only at higher concentrations. Applied for permeabilization of muscle fibres.
Abbreviation: Sap
MitoPedia topics:
Permeabilization agent
Application in HRR
Sap: Saponin (from quillaja bark); Sigma S 2149, 25 g, store at R.T.; FW = 1229.31
Caution: Prepare fresh everyday!
Comment: The product is withdrawn from Sigma!
Preparation of 5 mg/ml stock solution (dissolved in H2O):
- 1) weigh 5 mg of Saponin
- 2) dissolve in 1 ml H2O
→ use 50 µg Saponin/ml BIOPS: add 20 µl of 5 mg/ml saponin stock solution per 2 ml BIOPS
