Difference between revisions of "Smart Fluo-Sensor Green"
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{{Product | |||
|description='''Smart Fluo-Sensor''': LED with specified wavelength, photodiode, [[Filter-Cap]] attached with specific optical filter for the LED and/or photodiode. See [[Smart Fluo-Sensor Green]] and [[Smart Fluo-Sensor Blue]]. Smart Fluo-Sensors are precalibrated with sensor-specific memory and direct input into DatLab 7. | |||
|product id=xxx | |||
|product type=O2k-FluoRespirometer, O2k-Module | |||
|info= <--!'''''>> More details? Consult [[O2k-Fluo LED2-Module]]'''''--> | |||
|product image=[[image:SmartFluo.png|180px|right]] | |||
}} | |||
__TOC__ | |||
== O2k-Guide == | |||
=== Select the Smart Fluo-Sensors === | |||
:::: Switching between different excitation wavelengths and filters is achieved by simply exchanging the Smart Fluo-Sensors. Two types of optical sensors are supplied with different LEDs for fluorescence excitation, and the effective spectra of the LEDs are modified by filters. | |||
:::: Select the fluorescence sensor and filter set form the table on [[MiPNet17.05_O2k-Fluorescence_LED2-Module#Fluorophores|application-specific settings]]. Each fluorescence sensor is delivered with a mounted filter set: | |||
::::# [[Fluorescence-Sensor Green]]: 525 nm. The installed Filter-Cap is ready for H2O2 measurement with [[Amplex red|Amplex® UltraRed]]. | |||
::::# [[Fluorescence-Sensor Blue]]: 465 nm. The installed Filter-Cap is ready for measurements of mt-membrane potential with [[safranin]]. A different filter is used for measurement with [[Magnesium green| Magnesium green®]] or Calcium green® . | |||
'''O2k-Guide: Setup - next step [[Filter-Cap#Mounting_a_Filter-Cap|Mounting a Filter-Cap]]''' | |||
[[File:Fluorescence-Control Unit lettered.jpg|400px|right|link=]] | |||
=== Connect Fluorescence-Sensor to O2k === | |||
:::: For recording of fluorescence signals, insert the black sensor into the window of the O2k-Chamber as far as possible, aligning the Sensor-Guide Sector with the Sensor-Guide of the O2k-Front Fixation. Then connect the cable of the Fluorescence-Sensor to the Fluorescence-Sensor Plug of the Fluorescence-Control Unit. | |||
:::: Removing a Fluorescence-Sensor from the O2k chamber: Remove the Sensor-Guide to allow rotation of the sensor. Pull out the Fluorescence-Sensor while rotating it. Do not pull on the cable. Replace the Sensor-Guide. | |||
=== Stoppers === | |||
:::: Use only [[Stopper\black PEEK\conical Shaft\central Port|black PEEK stoppers]] in conjunction with fluorometric measurements. If necessary, replace the previous white PVDF stoppers. The black stoppers can be used for all HRR applications in general. See [[MiPNet19.18A_O2k-start]] for calibration of the O2k-chamber volume, which is identical for PEEK and PVDF stoppers. | |||
'''O2k-Guide: [[Fluorescence-Control_Unit#O2k-Guide|Electronic settings]]''' | |||
[[Category:Technical service]] | |||
Revision as of 12:01, 25 July 2017
Smart Fluo-Sensor Green
| Description | Smart Fluo-Sensor: LED with specified wavelength, photodiode, Filter-Cap attached with specific optical filter for the LED and/or photodiode. See Smart Fluo-Sensor Green and Smart Fluo-Sensor Blue. Smart Fluo-Sensors are precalibrated with sensor-specific memory and direct input into DatLab 7. |
|---|---|
| Product ID | xxx |
| Type | O2k-FluoRespirometer, O2k-Module |
| Link | <--!>> More details? Consult O2k-Fluo LED2-Module--> |
| Image |  |
O2k-Guide
Select the Smart Fluo-Sensors
- Switching between different excitation wavelengths and filters is achieved by simply exchanging the Smart Fluo-Sensors. Two types of optical sensors are supplied with different LEDs for fluorescence excitation, and the effective spectra of the LEDs are modified by filters.
- Select the fluorescence sensor and filter set form the table on application-specific settings. Each fluorescence sensor is delivered with a mounted filter set:
- Fluorescence-Sensor Green: 525 nm. The installed Filter-Cap is ready for H2O2 measurement with Amplex® UltraRed.
- Fluorescence-Sensor Blue: 465 nm. The installed Filter-Cap is ready for measurements of mt-membrane potential with safranin. A different filter is used for measurement with Magnesium green® or Calcium green® .
O2k-Guide: Setup - next step Mounting a Filter-Cap
Connect Fluorescence-Sensor to O2k
- For recording of fluorescence signals, insert the black sensor into the window of the O2k-Chamber as far as possible, aligning the Sensor-Guide Sector with the Sensor-Guide of the O2k-Front Fixation. Then connect the cable of the Fluorescence-Sensor to the Fluorescence-Sensor Plug of the Fluorescence-Control Unit.
- Removing a Fluorescence-Sensor from the O2k chamber: Remove the Sensor-Guide to allow rotation of the sensor. Pull out the Fluorescence-Sensor while rotating it. Do not pull on the cable. Replace the Sensor-Guide.
Stoppers
- Use only black PEEK stoppers in conjunction with fluorometric measurements. If necessary, replace the previous white PVDF stoppers. The black stoppers can be used for all HRR applications in general. See MiPNet19.18A_O2k-start for calibration of the O2k-chamber volume, which is identical for PEEK and PVDF stoppers.
O2k-Guide: Electronic settings
