Difference between revisions of "Smart Fluo-Sensor Green"

Latest revision as of 17:14, 16 March 2021

Smart Fluo-Sensor Green

MitoPedia O2k and high-resolution respirometry: O2k-Open Support

Description

Smart Fluo-Sensor Green: Excitation LED 525 nm (dominant wavelength) with short pass filter, emission (red) with long pass filter, individual sensors are calibrated with sensor-specific memory and direct input into DatLab 7 to obtain reproducible light intensities with different sensors, including photodiode, Filter-Cap equipped with Filter Set AmR for Amplex UltraRed and TMRM measurements when delivered.

Reference: Product details and purchase information

Additional resources

O2k-Fluo Smart-Module

Troubleshooting

-See: Defective fluorescence module

MitoPedia O2k and high-resolution respirometry: O2k hardware, O2k-Open Support, O2k-Respirometry, O2k-FluoRespirometry

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+{{Technical support}}
+__TOC__
+{{MitoPedia without banner
+|abbr=
+|description=[[Image:SmartFluo.png|200px|right]]'''Smart Fluo-Sensor Green''': Excitation LED 525 nm (dominant wavelength) with short pass filter, emission (red) with long pass filter, individual sensors are calibrated with sensor-specific memory and direct input into DatLab 7 to obtain reproducible light intensities with different sensors, including photodiode, [[Filter-Cap]] equipped with [[Filter Set AmR]] for [[Amplex® UltraRed|Amplex UltraRed]] and [[TMRM]] measurements when delivered.
+|info=[https://www.oroboros.at/index.php/product/smart-fluo-sensor-green/ '''Product details and purchase information''']
+}}
-{{Product
+== Additional resources ==
-|description='''Smart Fluo-Sensor''': LED with specified wavelength, photodiode, [[Filter-Cap]] attached with specific optical filter for the LED and/or photodiode. See [[Smart Fluo-Sensor Green]] and [[Smart Fluo-Sensor Blue]]. Smart Fluo-Sensors are precalibrated with sensor-specific memory and direct input into DatLab 7.
+:::*[[O2k-Fluo Smart-Module]]
-|product id=xxx
+== Troubleshooting ==
-|product type=O2k-FluoRespirometer, O2k-Module
+:::* ''-See'': [[Defective fluorescence module]]
-|info= <--!'''''>> More details? Consult [[O2k-Fluo LED2-Module]]'''''-->
+{{MitoPedia O2k and high-resolution respirometry
-|product image=[[image:SmartFluo.png|180px|right]]
+|mitopedia O2k and high-resolution respirometry=O2k hardware, O2k-Open Support, O2k-Respirometry, O2k-FluoRespirometry
 }}
-__TOC__
-== O2k-Guide ==
-=== Select the Smart Fluo-Sensors ===
-:::: Switching between different excitation wavelengths and filters is achieved by simply exchanging the Smart Fluo-Sensors. Two types of optical sensors are supplied with different LEDs for fluorescence excitation, and the effective spectra of the LEDs are modified by filters.
-:::: Select the fluorescence sensor and filter set form the table on [[MiPNet17.05_O2k-Fluorescence_LED2-Module#Fluorophores|application-specific settings]]. Each fluorescence sensor is delivered with a mounted filter set:
-::::# [[Fluorescence-Sensor Green]]: 525 nm. The installed Filter-Cap is ready for H2O2 measurement with [[Amplex red|Amplex® UltraRed]].
-::::# [[Fluorescence-Sensor Blue]]: 465 nm. The installed Filter-Cap is ready for measurements of mt-membrane potential with [[safranin]]. A different filter is used for measurement with [[Magnesium green| Magnesium green®]] or Calcium green®  .
- '''O2k-Guide: Setup - next step [[Filter-Cap#Mounting_a_Filter-Cap|Mounting a Filter-Cap]]'''
-[[File:Fluorescence-Control Unit lettered.jpg|400px|right|link=]]
-=== Connect Fluorescence-Sensor to O2k ===
-:::: For recording of fluorescence signals, insert the black sensor into the window of the O2k-Chamber as far as possible, aligning the Sensor-Guide Sector with the Sensor-Guide of the O2k-Front Fixation. Then connect the cable of the Fluorescence-Sensor to the Fluorescence-Sensor Plug of the Fluorescence-Control Unit.
-:::: Removing a Fluorescence-Sensor from the O2k chamber: Remove the Sensor-Guide to allow rotation of the sensor. Pull out the Fluorescence-Sensor while rotating it. Do not pull on the cable. Replace the Sensor-Guide.
-=== Stoppers ===
-:::: Use only [[Stopper\black PEEK\conical Shaft\central Port|black PEEK stoppers]] in conjunction with fluorometric measurements. If necessary, replace the previous white PVDF stoppers. The black stoppers can be used for all HRR applications in general. See [[MiPNet19.18A_O2k-start]] for calibration of the O2k-chamber volume, which is identical for PEEK and PVDF stoppers.
- '''O2k-Guide: [[Fluorescence-Control_Unit#O2k-Guide|Electronic settings]]'''
-[[Category:Technical service]]