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- Calabria 2017 MiP2017 WG4 + ([[Image:MITOEAGLE-logo.jpg|left|100px|link … [[Image:MITOEAGLE-logo.jpg|left|100px|link=http://www.mitoglobal.org/index.php/MITOEAGLE|COST Action MITOEAGLE]]</br>In our laboratory we are mainly focused on PBMCs blood cells. One of the main issues of working</br>with PBMCs is the quality of the final batch of cells. In particular the contamination of platelets in the final sample could affect the respiratory profile of the sample when evaluating mitochondrial</br>function. We have monitored all the steps of the PBMCs purification procedure with a Sysmex</br>XN-1000 hematology analyzer. This instruments allows a high degree of accuracy of platelets</br>counts, also using a specific fluorescent reagent [1]. The data collected show</br>that we achieve 97% depletion of platelets after two washing steps in RPMI, and we collect the</br>30% of the initial amount of PBMCs.</br></br>One of the main aim of [[WG4]] is also protocols in the harmonization to SUIT reference protocols</br>(RP1 and RP2). Since we are not currently running exactly the suggested RP protocols, we tried to verify if at least some common functional steps of the protocol are comparable to RP1 and RP2 protocols. Preliminary data show that at least in some common steps there is no significant</br>difference between the old protocol and the suggested RP1 and protocol in PBMCs human cells,</br>further experiments are needed to evaluate RP2.er experiments are needed to evaluate RP2.)
