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...nterest=Isolated mitochondria, Cultured cells, Intact cells, Permeabilized cells ...nterest=Isolated mitochondria, Cultured cells, Intact cells, Permeabilized cells

...nterest=Isolated mitochondria, Cultured cells, Intact cells, Permeabilized cells ...nterest=Isolated mitochondria, Cultured cells, Intact cells, Permeabilized cells

...aerobic energy metabolism in cultured cells. In: Energy transformations in cells and organisms'. Wieser W, Gnaiger E (eds), Thieme, Stuttgart:91-7. |tissues=Liver, Blood cells, Fat, Other cell lines, HeLa, Fibroblast, Lymphocyte

...nterest=Isolated mitochondria, Cultured cells, Intact cells, Permeabilized cells, Permeabilized muscle fibers ...nterest=Isolated mitochondria, Cultured cells, Intact cells, Permeabilized cells, Permeabilized muscle fibers

...vá M, Vištejnová L, Štengl M (2018) Beneficial effects of mesenchymal stem cells on adult porcine cardiomyocytes in non-contact co-culture. Physiol Res 67:6 ...ed no significant differences between viable CMCs from co-culture and CMCs cultured alone. In conclusion, non-contact co-culture of porcine MSCs and CMCs impro

...clopiazonic acid. Taken together, these results demonstrate that culturing cells under hyperoxic conditions reduces their ability to efficiently regulate [C |preparations=Intact cells

...n cultured and were immediately frozen at -80°C. One bulk culture of those cells had been permeabilized with digitonine before freezing, the other bulk was ...the process also HUH7 cells have been cultured and were frozen at -80°C in intact form as well.

...ena J, Neuzil J (2015) Evaluation of respiration of mitochondria in cancer cells exposed to mitochondria-targeted agents. Methods Mol Biol 1265:181-94. assess respiration in cultured cells, followed by the evaluation of the effect of one anticancer agent targeted

...drial function and response to oxidative stress in human neural progenitor cells. PeerJ 3:e1486. ...olism. Furthermore, antioxidant enzymatic activity was not altered in NPCs cultured in 3% oxygen under normal conditions, however, when exposed to external age

...omated method for isolating functionally intact mitochondria from cultured cells and tissue biopsies. Anal Biochem 443:66-74. ...isolate functionally active, intact mitochondria from cultured or primary cells and minute tissue samples in a rapid, highly reproducible manner.

...at the calorimetric-respirometric (CR) ratio does for our understanding of cells? Thermochim Acta 355:115-24. ...loric equivalent for the relevant catabolic substrate. The validity of the value for the CR ratio can be determined by Mayer’s enthalpy balance method. Hi

...idative phosphorylation increases the toxicity of SYD-1 on hepatocarcinoma cells (HepG2). Chem Biol Interact 256:154-60. ...s. These results show that SYD-1 is able to change the metabolism of HepG2 cells, and suggest that its cytotoxicity is related to impairment of mitochondria

...intact cultured neuronal cells; (2) by mitochondria isolated from HEK293T cells, and (3) by mitochondria isolated from mouse liver, which are useful as a g |preparations=Intact cells, Isolated mitochondria

...that 2 milions cells per chamber is enough for HRR measurements of SH-SY5Y cells (Figure 1). We did not observe the statistic significant difference in the |preparations=Intact cells

...iarities in disease settings that might not be evident in intact cells and allows for assessment of mitochondrial membrane integrity and sample purity.

...Culture at low glucose up-regulates mitochondrial function in pancreatic β cells with accompanying effects on viability. Islets 8:165-76. ...ic DNA). Thus culture at reduced glucose of pancreatic islets and clonal β cells leads to mitochondrial adaptions which possibly lessen the negative impact

...ning optimum concentrations for cell membrane permeabilization in cultured cells. |preparations=Intact cells, Permeabilized cells

...(1999) Detection of the changing substrate requirements of cultured animal cells by stoichiometric growth equations validated by enthalpy balances. J Biotec ...art of an overall aim to base the feeding of substrates to cultured animal cells on their actual metabolic needs,

|title=Welker L (2017) Metabolic and morphologic shifts in Neuro2a cells cultured in galactose medium. Master's Thesis p26. ...5-10, p < .05). Mitochondrial protein shows an increase in galactose-grown cells (n=3, p < .05).

...nergy provision in hepatocytes and that this effect was more pronounced on cells in culture than those in suspension. |preparations=Intact cells

...le=Asiri A (2017) Effects of asiatic acid on neurite outgrowth in Neuro-2a cells. Master's Thesis p42. ...ic acid on neuronal growth in murine neuroblastoma cells, Neuro2a. Neuro2a cells

...cell wall invertase. In conclusion, Al tolerance in cultured ''J. curcas'' cells involves a inhibition of mitochondrial AOX pathway, which seems to start an |preparations=Intact cells

...with ApoA1 for 4 hours. ROUTINE respiration reflects respiration in intact cells by growth medium substrates. ET capacity reflects the maximal capacity of o |keywords=ApoA1, Cell respiration, Leukocyte, Jurkat cells, Biomarker, High-resolution respirometry

...ndrial ATP synthesis, respiration, isolation of mitochondria from cultured cells and tissues, and OXPHOS enzymology. We also explain common pitfalls, guide |tissues=Nervous system, Blood cells

|title=Djafarzadeh S, Jakob SM (2017) Isolation of intact mitochondria from skeletal muscle by differential centrifugation for High-R ...solation procedure and that large amounts of the tissue biopsy or cultured cells are required for the mitochondrial isolation.

...genation on the mitochondrial function and the free radicals production of cultured primary equine skeletal myoblasts. Res Vet Sci 95:870-8. ...he exercise can cause excessive production of free radicals. This study on cultured primary equine myoblasts investigated the effect of different kinds of anox

...uces oxidative phosphorylation by activating cytochrome c oxidase in human cultured neurons and astrocytes. Oncotarget 7:7426-40 ..., this is the first study showing that EGCG promotes CcO activity in human cultured neurons and astrocytes. Considering that CcO dysfunction has been reported

.... RNA sequencing confirmed that gene expression differences between islets cultured in suspension and hydrogel largely fell within gene ontology terms related |preparations=Intact cells

...h the accumulation and subsequent oxidation of (14)C-labeled substrates in cultured human myotubes, adipocytes, and hepatocytes. Both methods are adaptable for

...50</sub>) in intact coupled cells and in digitonin-permeabilized uncoupled cells. In both cases, the P₅₀ in patients was elevated 2.1- and 3.3-fo |preparations=Intact cells

...ant oxygen tensions differentially regulate hepatotoxic responses in HepG2 cells. https://doi.org/10.1016/j.tiv.2021.105156 ...gen tensions better matched primary human hepatocyte data than HepG2 cells cultured under standard conditions. Despite altered transcriptional regulation with

...t SKN-SH cells have higher spare respiratory capacity compared to SH-SY 5Y cells. |preparations=Intact cells

...ndrial density and mild uncoupling of respiration. Importantly, only cells cultured in HPLM displayed mitochondrial dysfunction upon exposure with the clinical ...dia, cell proliferation, cell growth, nutrients, media formulation, living cells, high-resolution respirometry, coupling control, ROUTINE respiration, LEAK

...interleukine-6 of human amniotic mesenchymal stromal cells in vitro. Stem Cells Int 2018:9502451. |journal=Stem Cells Int

...in some studies of intracellular processes due to the presence of blood in intact vertebrates. ...a, Wax worms, Sulfide, Cyanide, Azide, Bovine pulmonary artery endothelial cells, BPAEC

...tion of free radicals and oxygen consumption by primary equine endothelial cells during anoxia-reoxygenation. Open Biochem J 5:52-59. ...ese results show that A/R induces mitochondrial alterations in endothelial cells, and strongly stimulates their oxidative activity as demonstrated by ESR an

...hrome c oxidase in wild-type and mitochondrial DNA mutation-carrying human cells. Proc Natl Acad Sci U S A 94:1166-71. ...ondrial DNA mutation-carrying human cell lines. Surprisingly, in wild-type cells, only a slightly higher COX capacity was detected than required to support

...ion was measured using a Clark-type oxygen electrode. Levels of ATP in the cells were determined according to the luciferin-luciferase method. Cellular synt RESULTS: Respiration and ATP synthesis of cultured chondrocytes were inhibited by NO, particularly under low oxygen concentrat

...substrate succinate cannot support respiration when administered to intact cells due to inadequate cell membrane permeability. We introduce a group of new c ...ssayed using high-resolution respirometry of intact human peripheral blood cells. Several protocols were used – with and without rotenone inhibition of CI

...ls are widely understood to significantly impact the phenotype of cultured cells in the abstract, in practise the importance of the above parameters does no |preparations=Intact cells

...btree-like effect. These results show that glycolysis predominated in heme-cultured epimastigotes over oxidative phosphorylation for energy supply when glucose |preparations=Intact cells

...996) Oxygen dependence of respiration in coupled and uncoupled endothelial cells. Am J Physiol Cell Physiol 271:C2053-61. https://doi.org/10.1152/ajpcell.19 ...ls. Therefore 70 % of the flux dependence of ''p''₅₀ in coupled cells was caused by changes in metabolic state, which correlated with respiratory

...on, reduced proliferation, autophagy, and cell degradation. The ability of cells to tolerate this type of stress may be important in toxicity and chemoresis |tissues=Blood cells

...alemma and the mitochondrial membrane. Phototoxicity assays of PDT-treated cells carried out under different partial pressures of oxygen showed dose-depende |preparations=Intact cells

...most cytotoxic of reactive oxygen species (ROS), and effectively protected cells; however, H₂ did not react with other ROS, which possess physiol |preparations=Intact cells

|title=Gnaiger E, Kemp RB (1990) Anaerobic metabolism in aerobic mammalian cells: information from the ratio of calorimetric heat flux and respirometric oxy ...tain an anaerobic contribution to their total heat flux. In many mammalian cells this can be explained quantitatively by lactate production observed under f

|preparations=Intact organ, Intact cells ...tion of intracellular Ca²⁺ homeostasis in EA.hy 926 endothelial cells.

...xplant of the rat embryonic spinal cord is placed on a monolayer of muscle cells. In those co-cultures motor neurons functionally innervate myotubes which t |preparations=Intact cells

...tion affect insulin signaling and action in cultured human skeletal muscle cells? Am J Physiol Endocrinol Metab 294:E97-102. ...ntreated cells. This increased to 669 +/- 69 and 823 +/- 83 pmol/min/mg in cells treated with 50 and 75 microM azide, respectively (vs. untreated, both P <

...rage of the mitochondrial proteome of crude and purified mitochondria from cultured liver cancer cell lines.

:::: '''MitoEAGLE data repository for blood cells and clutured cells''' ::::» [[WG4 MitoEAGLE data: blood and cultured cells |WG4 Action]]

...mage response that ultimately leads to premature senescence of susceptible cells. Our findings provide a mechanism for integrating metabolic effects of THs |preparations=Intact cells

..., Arroyo V, Clària J (2023) Essential role for albumin in preserving liver cells from TNFα-induced mitochondrial injury. https://doi.org/10.1096/fj.2022015 ...hat the albumin molecule is required for the effective protection of liver cells from mitochondrial oxidative stress induced by TNFα. These findings emphas

.... A decrease in the mitochondrial membrane potential (MMP) was observed in cultured hepatocytes treated with tBHP, as illustrated by a significant reduction in |preparations=Intact cells, Permeabilized cells, Isolated mitochondria

...perfusion: mitochondrial respiratory defects in cultured human endothelial cells. Transplantation 74:1800-3. ...a useful model to study the mechanisms of CIR injury to human endothelial cells, whereas the full pattern of CIR injury could not be induced by a pulse of

...ková A, Ježek P (2020) Glucose-induced expression of DAPIT in pancreatic β-cells. Biomolecules 10:E1026. ...whereas net GSIS rates accounted for 80% and 90% in USMG5/DAPIT-deficient cells. Consequently, the sufficient DAPIT expression and complete ATP synthase as

...promotes mitochondrial biogenesis in human pulmonary bronchial epithelial cells. Biochem Biophys Res Commun 515:31-36. ...ne is a potent metabolic inducer of mitochondrial biogenesis in epithelial cells. Aminophylline could have a therapeutic effect on epithelial mitochondrial

...ochondrial transfer enhances recovery from models of reperfusion injury in cultured cardiomyocytes. J Cell Mol Med 24:5007-20. ...reviously reported uptake of isolated exogenous mitochondria into cultured cells through co-incubation, mediated by macropinocytosis. Here, we report the us

...ork characteristics in C2C12 mouse myoblasts and PC3 human prostate cancer cells under conditions of physiological (5 %) and supra-physiological (18 %) oxyg |preparations=Intact cells

...lture medium for the growth and productivity of genetically engineered CHO cells. Cytotechnol 30:107-20. ...ors to monitor and control on-line and in real time the growth of cultured cells. Since growth is accompanied by an enthalpy change, heat dissipation measur

...intain the normal biogenesis of this respiratory complex in cultured human cells. |preparations=Intact cells

Human Caco-2 cells were cultured for 10 days in culture flasks or Caco-2 cells cultured in galactose-containing, but not in glucose-containing,

...trate use in mitochondria. Additionally, explants of placental tissue were cultured for four, 12, 24, 48, or 96 h and respiration measured. ...ptible to changes in respiratory function relative to first trimester when cultured ''in vitro'', perhaps reflecting changes in metabolic demands as gestation

...d fatigue. Using fibre-type-specific promoters, we show in cultured muscle cells that [[PGC-1alpha]] activates transcription in cooperation with Mef2 protei |preparations=Intact organism, Intact cells

...heterozygous deletion of ''Sod2'' did not accelerate the depletion of stem cells and the impairment in organ maintenance in aging ''mTerc‐/‐'' mice. In ...eroxide, Telomere shortening, Aging, DNA damage, SOD2, Free radicals, Stem cells

...ake, whereas mitochondrial respiration and membrane potential remain fully intact. MCU has two predicted transmembrane helices, which are separated by a high |preparations=Intact cells, Permeabilized cells, Permeabilized tissue, Isolated mitochondria

...ea pigs displayed reduced respiration compared with NBW/CD. Cultured C2C12 cells were utilized to better understand independent effects of hypoxia and fatty |preparations=Permeabilized tissue, Intact cells

...more detailed understanding of the basic molecular alterations in leukemic cells induced by reactive oxygen species. Acute lymphoblastic leukemic cell lines Jurkat and CCRF-SB were cultured according to standard operating procedures and exposed to a single bout of

...ed human articular chondrocytes and immortalized costal chondrocytes (TC28 cells). We also assessed the effects of antimycin A and oligomycin (inhibitors of ...oprotein 1 (PC-1) to increase chondrocyte PPi generation. Finally, MV from cells treated with antimycin or oligomycin contained less PPi and precipitated >5

...fusion via OPA1 and MFN1 supports liver tumor cell metabolism and growth. Cells 9:E121. |journal=Cells

...han rats treated with vehicle. ''In vitro'' analysis on cultured EA.hy 926 cells demonstrated that pHPP improved cell growth rate and promoted cell survival |preparations=Intact cells

:: e) [''Y/N'']: Permeabilized cells :: f) [''Y/N'']: Intact cultured cells (fibroblasts, ..)

:: e) [''Y/N'']: Permeabilized cells :: f) [''Y/N'']: Intact cultured cells (fibroblasts, ..)

.../sub>-pumping is impaired. Enzyme inactivation by the detergent in patient cells indicates instability of incomplete CIV assemblies. |preparations=Intact cells, Permeabilized cells

...iakis et al 2014). Since cultured cells are routinely stored at -80 °C and cultured after thawing, it appears most promising to optimize conditions for cryopre

...ber S, Aichler M, Zischka H (2015) Isolation of mitochondria from cultured cells and liver tissue biopsies for molecular and biochemical analyses. Methods M |abstract=We recently reported a new method to isolate functionally intact mitochondria from cell culture and small tissue samples (Schmitt et al., An

...ntent on metabolic properties of renal epithelial cell cultures. Are renal cells in tissue culture hypoxic? https://doi.org/10.1159/000016312 ...ontent-on-metabolic-properties-of-renal-epithelial-cell-cultures-Are-renal-cells-in-tissue-culture-hypoxic-Cell-Physiol-Biochem.pdf Open Access]

...andidates, we designed ''in vitro'' assays using equine skeletal myoblasts cultured from muscle biopsies and subjected to toxins involved in atypical myopathy. |preparations=Permeabilized cells, Intact cells

...fect as an early event in preservation/reoxygenation injury in endothelial cells. Transplantation 63:136-42. ...y, of controls (46.5±3.3 pmol O₂·s^-1·10^-6 cells), indicating significantly better protection by UW solution than HTK soluti

...ared to L0 and LC. This protection against mitochondrial dysfunction in LI cells was associated with lower activity of mechanistic target of rapamycin compl |preparations=Intact cells, Isolated mitochondria

...rved between attached and suspended cells in ROUTINE respiration of living cells and LEAK respiration obtained after inhibition of ATP synthase by oligomyci |keywords=attached cells; ace, suspended cells; sce, fibroblasts, high-resolution respirometry; HRR, Oroboros O2k, Seahors

...hology) in intact living primary human skin fibroblasts (PHSFs). Cells are cultured in 96-well plates and stained with tetramethyl rhodamine methyl ester (TMRM

...n. In this study we investigated the effect of Hg(II) (HgCl(2)) on GJIC in cultured human keratinocytes. It is shown that subcytotoxic concentrations of HgCl(2 |preparations=Intact cells

...ifferent ''in vitro'' sensitivity to ceftriaxone among patient and control cells, suggested that the mitochondrial disease symptoms were hastened by the inf |preparations=Intact cells

...(PTPases)-upstream factor of AKT. Interestingly, PTEN knockdown sensitized cells to VAOS, whereas ectopic expression of PTEN markedly rescued VAOS-mediated ...te oligosaccharides, A549 adenocarcinomic human alveolar basal epithelial cells

...expression levels. In conclusion the addition of EGCG to DHA returned the cells to the control conditions in terms of mitochondrial morphology, energy and |preparations=Intact cells

...whole and permeabilized neurons from 9-month and 24-month adult rat cortex cultured in common conditions. After permeabilization, respiration increased in both |preparations=Intact cells, Permeabilized cells

...metabolism and induces sensitivity to menadione in cultured tumor-derived cells. Cancer Metab 9:3. ...ed (in part) by variation in the metabolic phenotypes of the tumor-derived cells that can be induced by selective adaptation to subculture conditions.

...a-Kiec A, (1010) The protective effect of L-arginine in LN-18 glioblastoma cells. FENS Abstr 5:050.67. Methods: The cultured glioblastoma LN-18 cells were preincubated with L-arginine (L-arg; 0.1, 0.3 or 1 mM) for 24 hours an

...te. Effects of direct nitrite exposure on cell respiration were studied in cultured human primary myotubes. |tissues=Skeletal muscle, Stem cells

...ka-Kiec A (2010) The protective effect of L-arginine in LN-18 glioblastoma cells. FENS Abstr vol.5,050.67. Methods: The cultured glioblastoma LN-18 cells were preincubated with L-arginine (L-arg; 0.1, 0.3 or 1 mM) for 24 hours an

...e damage induced by ''tert''-butyl hydroperoxide (tBHP) in rat hepatocytes cultured in suspension and in monolayer. At the lowest (0.1 mM) concentration, decre |preparations=Intact cells

...as alternative permeabilization agent in respirometric assays of cultured cells and isolated synaptosomes, superior to digitonin in its tolerability for mi |preparations=Permeabilized cells, Isolated mitochondria, Intact cells

...ucose concentration in cell culture media on hepatocellular carcinoma HuH7 cells evaluated by high-resolution respirometry. ...ed to study coupling control and electron-transfer (ET) capacity in living cells.

...ondrial function and reactive oxygen species in porcine aortic endothelial cells. Am J Physiol Endocrinol Metab 298:E89-98. ...nd the activities of key enzymes in the mitochondrial respiratory chain of cultured porcine aortic EC. Oxygraphy detected that eoLDL significantly reduced oxyg

...fied by oxygen polarography and applied in isolated mitochondria, cultured cells, and permeabilized fibers derived from human or animal tissue biopsies. Tra |preparations=Intact organism, Isolated mitochondria

...on of HIF-1α by adenosine-A_2B R signaling in epicardial stromal cells formed after myocardial infarction. FASEB J 35:21517. ...ivation and hypoxia-hypoxia-inducible factor 1 alpha (HIF-1α) signaling in cultured EpiSC, isolated from rat hearts 5 days after MI. We found substantial nucle

...ondria on the response of highly proliferative and invasive bladder cancer cells to the combined inhibition of mTOR and SIRT1. ...ria mediates the positive response of highly proliferative and invasive BC cells to the combined inhibition of mTOR and SIRT1.

|title=Mitochondrial function of cyropreserved HEK 293T cells: development of a reference sample for high-resolution respirometry. ...lity and stabilize respiratory characteristics of intact and permeabilized cells over variable periods of time.

...with identical exposures. In both murine tissues and cultured endothelial cells, expression of mutant Bmpr2 was sufficient to cause oxidant injury that was |preparations=Intact cells

...family of non-syndromic HON and of functional analyses in patient-derived cultured skin fibroblasts and the yeast ''Yarrowia lipolytica''. ...causing mutations (p.Tyr53Cys; p.Tyr308Cys). Studies using patient-derived cultured skin fibroblasts revealed mildly decreased ''NDUFS2'' and complex I abundan

...[2]. Studies conducted in mouse liver, hamster lung and human hepatocytes cells reported that amiodarone mitochondrial toxicity was caused by an impairment ...ties of intact and digitonin-permeabilized platelets (200 x 10⁶ cells/mL) were analyzed using the substrate-uncoupler-inhibitor titration protoco

...amide treatment reduced mitochondrial O2 consumption (i.e. respiration) in cultured myotubes and permeabilized red gastrocnemius muscle fibre bundles. Ceramide |preparations=Intact cells, Permeabilized tissue

...el E (2014) Characterization of human bone marrow derived mesenchymal stem cells as a model for ''in vitro'' adipocytes studies. PhD Thesis 1-106. ...s increased during adipocyte differentiation. Oxygen consumption of intact cells was measured with a Seahorse flux analyzer. Basal respiration as well as le

...nes and pathways, whereas functional assessment in both cardiac tissue and cultured cardiomyocytes indicated that oxidative metabolism decreases between the fi |preparations=Intact cells

...er body mass index and tended to have less muscle mass. In cultured muscle cells, mitochondrial protein content and myosin heavy chain isoform I (but not II |preparations=Intact cells

...nd mitochondrial Ca²⁺ homeostasis in the vascular smooth muscle cells. TRPM8 activation by menthol antagonized angiotensin II induced mitochondri |preparations=Intact cells, Permeabilized cells

...ow offers the opportunity to measure oxygen consumption in intact cultured cells. Magnetic resonance spectroscopy provides the most direct way of assessing

....jpg|left|90px|Ana Bastos Sant'Anna]] Role of succinate in prostate cancer cells: uptake and mitochondrial respiratory function. ...ts mitochondrial utilisation is increased in permeabilized prostate cancer cells [3].

...e body surface. Serum was collected 24 hours post injury. HepG2 cells were cultured with serum-enriched media from either sham or burn treated rats. Protein le ...liver increased after burn by 17% and 14%, respectively. Exposure of HepG2 cells to serum from burned rats increased the pAMPKα:AMPKα ratio (p < 0.001

...TOEAGLE|COST Action MITOEAGLE]] Metabolic studies in human skeletal muscle cells. [[Media:Rustan Presentation Mitoeagle final.pdf|Rustan_Presentation]] ...les, with special attention to changes in muscle energy metabolism between cells established from various donors, after different treatments of the myotubes

...sue samples from animal models and human patients. Using cultured cells or cells extracted from blood samples, the effects of genotypic variations or of var ::::* coupling control protocols (CCP) in intact cells to evaluate effects on ROUTINE respiration, biochemical coupling efficiency

...iques (Oroboros-O2k) allowing the study of mitochondrial function in blood cells. Based on the experience and knowledge that I will get, it could lead to th ...ccess to specimens from human subjects, which will allow me to address the value of obtained results as biomarkers for mitochondrial dysfunction and linked

...ob SM (2012) Effect of remifentanil on mitochondrial oxygen consumption of cultured human hepatocytes. PLoS One 7:e45195. ...rior remifentanil incubation prevented TNF-α-induced IL-6 release of HepG2 cells, and attenuated fragmentation of pro-caspase-3 into cleaved active caspase

...ondrial respiratory capacity in IL-1ß treated human peritoneal mesothelial cells. Cell Biochem Biophys 44:179-86. ...y chain, but is associated with a decrease of mitochondrial content of the cells that is correlated with an increase in LDH (and thus glycolytic) capacity.

...several parameters of mitochondrial function from adherent intact cultured cells were measured. Western blotting analysis was performed for detection of the ...increased the intracellular ROS production and apoptotic rate of the LoVo cells, while the expression of apoptosis inducer, p53 and caspase-3 also increase

...direct manipulation of mitochondrial calcium by overexpression of MCUb in cultured adipocytes was able to alter a number of metabolic parameters, including mi |preparations=Intact cells

...ue in the ''in vivo'' studies, in translational research easily accessible cells that could possibly act as the indicator(s) of these changes are looked for ...buffer, and then homogenized. The mitochondrial respiration parameters in intact blood platelets were monitored with the use of O2k high-resolution respirom

...al and endoplasmic reticulum morphology, as well as the mtDNA content in a cultured primary fibroblast obtained from a CMT2A patient harboring a ''de novo'' Ar |preparations=Intact cells

...g an increase in proton leak. Next, we assessed respiration in MII oocytes cultured for 8 h. The aged oocytes had a significantly reduced maximum respiratory c |preparations=Intact cells

...al and endoplasmic reticulum morphology, as well as the mtDNA content in a cultured primary fibroblast obtained from a CMT2A patient harboring a ''de novo'' Ar |preparations=Intact cells

...metformin and rotenone prevented ionomycin-induced MPTP opening in intact cells. The data suggest that, depending on the age, phenformin and metformin may

...n concentrations for complete plasma membrane permeabilization of cultured cells can be determined directly in a respirometric protocol (see: [[SUIT-010 O2 ::::# Incubate cells in the O2k in mt-respiration medium (MiR05 or [[MiR06]]) and measure ROUTIN

.... From a technical standpoint, BAM15 represents an effective new tool that allows the study of mitochondrial function in the absence of off-target effects th |preparations=Intact organism, Intact cells

...nflammatory mediators (IM) that were obtained by incubation of white blood cells with LPS. MitoTempo, a mitochondria targeted antioxidant, was used to clari Our data suggest that inflammation mediated elevation of mtROS in liver cells reduce membrane potential without significant impairment of mitochondrial f

...ith age. Neurons isolated from the brains of 24 month and 9 month rats and cultured in common conditions provide a model of intrinsic neuronal aging. ''In situ |preparations=Intact cells

...tochondrial uncouplers in cardiomyocytes. Neonatal rat cardiomyocytes were cultured. Protein levels were measured by using western blot technique. The whole ce |preparations=Intact cells

...f "glucose-dependent" insulin resistance. Using primary rat adipocytes and cultured 3T3-L1 adipocytes, we observed that insulin increased respiration, but nota |preparations=Intact cells

...lood in vivo at high concentration in peripheral tissues [5]. Furthermore, cells are chronically exposed to these compounds for long periods of time which i ...e assay. In addition, high-resolution respirometry on corresponding intact cells enabled the measurement of mt respiration and analysis of oxidative phospho

...hology) in intact living Primary Human Skin Fibroblasts (PHSFs). Cells are cultured in 96-well plates and stained with tetramethyl rhodamine methyl ester (TMRM |preparations=Intact cells

...se in oxidative glucose metabolism. We speculate, that expression of hGDH2 allows astrocytes to spare glucose and utilize BCAAs during substrate shortages. T |preparations=Enzyme, Intact cells

...y labelled as ''intact cells'' in the sense of the total cell count, but ''intact'' may suggest dual meanings of ''viable'' or unaffected by a disease or mit ::::* ce - [[living cells]]

...respiratory control ratio and spare respiratory capacity) was aberrant in cultured sensory neurons from streptozotocin-diabetic rats and was corrected by resv |preparations=Intact cells

...ing the size and increasing the number of this organelle in C6 astroglioma cells. However, this phenomenon that points to increased mitochondria fission, wa ...ites (MG and AGEs) disrupts mitochondrial dynamics and physiology in nerve cells. The phenomenon might induce cell death, and therefore, a higher predisposi

...ecreased by 44.4% ± 9.8%. Respiratory depression by rotenone suggests that cultured myobundles rely heavily on the complex I pathway for ATP synthesis during t |preparations=Intact cells

species in cultured human vascular endothelial cells. When Mt are Intact placental Mt were isolated from of NP and PE

...he mtPT. Similarly, salicylate induces a CsA-sensitive mtPT and killing of cultured hepatocytes. These in vitro findings suggest that the mtPT is the pathophys |preparations=Intact cells, Isolated mitochondria

...the observed decrease in oxygen affinity with increasing metabolic rate in cells. In addition, mitochondrial oxygen affinities decrease from the resting to ...tions=Isolated mitochondria, Enzyme, Oxidase;biochemical oxidation, Intact cells

...s mediated silencing of the FAHD1 gene in Human umbilical vein endothelial cells (HUVEC) [3]. Moreover, it is known that the FAHD1 gene is amplified in a su ...d by lentivirus infection in the breast cancer cell line MCF-7. Cells were cultured in DMEM containing both, pyruvate and L-Glutamine or none. Cell number was

...invasive then a collection of other tissues, b) there is a large number of cells in blood that are easy to isolate, c) blood circulating in the bloodstream ...ry without further processing. The mitochondrial respiration parameters in intact blood platelets were monitored with the use of high-resolution respirometry

...daptation of iPD to a simulated pre-diabetogenic state, we exposed primary cultured fibroblasts from iPD patients and controls to standard (5 mM) and high (25 |preparations=Permeabilized cells, Intact cells

...ithin 3 h after CLP. The decrease of cytokine expression was reproduced in cultured macrophages after exposure to HBOT. Early HBOT could be of benefit in the t |preparations=Intact cells

...ed by decreased respiratory capacity and elevated superoxide production in cultured myotubes. |preparations=Isolated mitochondria, Intact cells

...d mitochondrial protein translation are deficient in patients' fibroblasts cultured in the absence of glutamine but restore in high glutamine. Lentiviral rescu |preparations=Permeabilized cells, Intact cells

...olic acid delays aging in yeast and exhibits an anti-tumor effect in human cells by altering mitochondrial composition, structure and function. Mitochondr P ...cancer cell cultures are opposite of those observed in non-cancerous human cells and in chronologically aged, quiescent yeast.

...cellular physiology, as the range of ''p''_c values in isolated cells, e.g. passive and active myocytes (31). The microxic region thus incorporat ...erion for the conforming pattern. The plot may terminate at some arbitrary value without further attention as to the shape at low oxygen levels (Fig. 1; cur

...to oxygen radical production rather than the kinetics of CIV. In cultured cells, the pronounced oxygen uptake above mitochondrial saturation at air-level o ...pmol·s^-1·Mx^-1 || pmol·s^-1·10^-6 cells

...egradation of existing protein, caused Nrf2 to accumulate significantly in cells from young animals (p < 0.05), but not old, indicating a lack of new Nrf2 s ...i-quinone. GSH declined by a 3-fold greater margin in old versus young rat cells given 300 µM menadione (p<0.05 and p≤0.01 respectively; N=3), and provid

=== RP1 and RP2 in cells === ...luate respiration in two different modules: intact cells and permeabilized cells. ROUTINE respiration (ce1) is obtained before digitonin is employed, wherea

...of ruminants. Hutchinson, London: 332 pp [1]). In many cultured mammalian cells, aerobic glycolysis contributes to total ATP turnover ([[Gnaiger_1990_Bioch |preparations=Intact cells, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria

...horylation in freshly isolated mitochondria and in other preparations with intact, fresh mitochondria. ...Similar type of measurements can be done in cultured skin fibroblasts and cells harvested from blood.

...we analyse mitochondrial function using several basic models: hepatocytes cultured ''in vitro''; mitochondria in permeabilised hepatocytes; tissue homogenates |preparations=Intact cells, Permeabilized cells, Homogenate, Isolated mitochondria

...tration]] (SUIT) protocols with [[isolated mitochondria]], [[permeabilized cells]] or tissues, in particular permeabilized muscle fibres [4]. Physiological ..., ''(2)'' the amount of biological material is limited (<0.5 mill cultured cells, 1-2 mg of fresh tissue from biopsies; <0.05 mg of mitochondrial protein),

...retation of various experiments on isolated mitochondria and permeabilized cells and tissue or homogenates. It is important to provide validated guidelines * calcium insults in intact and permeabilised cells;

...ic study tasks for mitochondrial respiratory studies in blood and cultured cells, ...itoEAGLE_data:_blood_and_cultured_cells|MitoEAGLE data: blood and cultured cells]]

* '''Bioblast news''': '''Light in the powerhouse of the cells''' - [[Houska Award 2012| a mt-project is the winner of the Houska Award 20 ...cultured cells, including human pluripotent stem cells and differentiated cells Nat Protoc 7: 1068-85.]] >> ''Continue the [[O2k_versus_multiwell_respirome

...ich contains HGA, MCPA and a general increase in acylcarnitine species) on cultured equine myoblasts depresses NS-linked respiration. Secondly, we have showed ...for evaluating the effectiveness of selected therapeutics assumed to be of value to prevent and/ or counteract the mitochondrial dysfunction associated to H

| High-resolution respirometry in blood cells and cell lines | Investigation of mitochondrial function in blood cells by high-resolution respirometry (Orosboros NextGen-O2k)

...'single individual cell'. A cell count is the multitude or number ''N'' of cells, ''N''_ce = ''N''·''U''_ce ([[Gnaiger MitoFit Preprint :::: Cells are frequently pipetted into the O2k-chamber. Start with chamber and stoppe

...tudents, the team will share expertises and work synergistically along the value creation chain to address the unmet medical need of more informative NAFLD ::::* Testing activation of Kupffer cells by mitochondrial protein components

...uding isolated mitochondria, intact and permeabilized cultured and primary cells) for future QA workshops.

...r E (2018) High-Resolution FluoRespirometry and OXPHOS protocols for human cells, permeabilized fibers from small biopsies of muscle, and isolated mitochond

...can be used immediately for respirometry of both intact and permeabilized cells. Thus interlaboratory proficiency testing may now be feasible as a world-wi ...O2k-demo experiment: reference SUIT protocol with cryopreserved mammalian cells'''

...Comparative study of respiration in Atlantic salmon (''Salmo salar'', L.) cells and mitochondria from blood, heart, liver, muscle and brain]]||A2-06 Poster ..., US||[[Hendricks 2013 Abstract MiP2013|Bioenergetics of permeabilized and intact nerve cell terminals from ApoE deficient and wild type mice]]||C3-07 Poster

...possess functional characteristics that differ fundamentally from those of intact mitochondria in permeabilized myofibers. Our work and that of others unders ...tative of in vivo mitochondrial arrangement than isolated mitochondria and allows determination of mitochondrial functional parameters in small endomyocardia

...5 mM ADP, yet few studies use such high [ADP] in permeabilized tissues and cells. - Oxygen limitation of respiration below air saturation. [[File:Renner_200 ...tochondrial content and cell size after induction of apoptosis in leukemia cells. Biochim Biophys Acta 1642:115-23. - [[Renner 2003 Biochim Biophys Acta |»

...ject: Coordination of glutaminolysis and glycolysis in PC3 prostate cancer cells. - [[Media:20190711_Abstract_MitoFitOpenSeminar_DiPaolaFJ.pdf |»Abstract« ...acellular coenzyme Q10 (CoQ10) is reduced to ubiquinol-10 by intact Hep G2 cells independent of intracellular CoQ10 reduction", [https://www.ncbi.nlm.nih.go

...is associated with increased mitochondrial bioenergetic function of immune cells in mothers, but not in their newborns'''<br> ...ges in mitochondrial bioenergetics in maternal, but not in neonatal immune cells. Proc Natl Acad Sci U S A 117:24778-84. [[Gumpp 2020 Proc Natl Acad Sci U

...stematic unit of bioenergetics and chemiosmotic coupling studied in living cells and mitochondrial preparations. A rigorous understanding of mitochondrial r ...eaning of respiratory states and rates, from ROUTINE respiration of living cells to the capacity of oxidative phosphorylation (OXPHOS) determined in mitocho

...ss]], [[Normalization of rate]], [[Object]], [[Organism]], [[Permeabilized cells]], [[Publish]], [[Sample]], [[System]], [[Unit]], [[Volume]], ...g-house is an abstract number of lifeless monetary units, or the numerical value of a dead mass of gold. The message of ''A Christmas Carol'' is emotional a

...two methoxy groups and one methyl group being present in all tissues and cells. In humans, CoQ₁₀ occurring in the mitochondrial electron transf ROUTINE respiration is the respiratory activity of living (non-permeabilized) cells in the physiological coupling state, which is controlled by cellular energy

...is associated with increased mitochondrial bioenergetic function of immune cells in mothers, but not in their newborns'''<br> ...ges in mitochondrial bioenergetics in maternal, but not in neonatal immune cells. Proc Natl Acad Sci U S A 117:24778-84. [[Gumpp 2020 Proc Natl Acad Sci U