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{{MitoPedia without banner |description=[[Image:ISS-Filter and Tubing.JPG|right|180px]]'''ISS-Filter and Tubing''', [[ISS-Integrated Suction System]].

{{MitoPedia without banner ...rial membrane potential with [[Safranin]] when delivered. The filter set [[Filter Set MgG / CaG]] for [[Magnesium green]]® / [[Calcium green]]® measurement

{{MitoPedia without banner ...equipped with [[Filter Set AmR]] for [[Amplex® UltraRed|Amplex UltraRed]] and [[TMRM]] measurements when delivered.

{{MitoPedia without banner |description=[[Image:TIP2k Filter Papers.JPG|right|180px]]'''TIP2k Filter Papers''' (package of 10) to be used for the stoppers during TIP2k titratio

{{MitoPedia without banner ...mine 123|rhodamine 123]]. [[Filter Set MgG / CaG]] for [[Magnesium green]] and [[Calcium green]] measurements are included.

{{MitoPedia without banner ...D 525 nm (dominant wavelength), photodiode, [[Filter-Cap]] equipped with [[Filter Set AmR]] for [[Amplex® UltraRed|Amplex UltraRed]] measurements when deliv

{{MitoPedia without banner ...orescence-Sensor Green]]. The filter set consists of 6 LED filters (round) and 6 photodiode filters (rectangular).

{{MitoPedia without banner ...Smart Fluo-Sensor Blue]]. The filter set consists of 6 LED filters (round) and 6 photodiode filters (rectangular).

{{MitoPedia without banner ...Smart Fluo-Sensor Blue]]. The filter set consists of 6 LED filters (round) and 6 photodiode filters (rectangular).

{{MitoPedia without banner ...nge of optical filters for the optical pathways from the LED to the sample and from the sample to the photodiode.

{{MitoPedia without banner ...ganisms, various poisons (inhibitors, uncouplers) and mixtures of proteins and substrates are safely disposed off in the 2-litre waste bottle.

{{MitoPedia without banner ...mittent by default, or all information. The experimental log can be viewed and saved as a PDF file by clicking on [Preview].

{{MitoPedia ...e parameters: the [[response time of the POS]], the data sampling interval and the number of points used for flux calculation.

{{MitoPedia without banner ...Ca²⁺ (Calcium Green™), and numerous other applications open for O2k-user innovation.

{{MitoPedia without banner ...Ca²⁺ (Calcium Green™), and numerous other applications open for O2k-user innovation.

|title=Fasching M, Sumbalova Z, Gnaiger E (2012) O2k-Fluorometry - a ''MitoCom'' project. Mitochondr Physiol Network 17.12. ...al membrane potential, intracellular pH, Ca²⁺, Mg²⁺, and NADH levels.

{{MitoPedia == Calcium Green in high-resolution respirometry ([[HRR]]) ==

{{MitoPedia without banner ...ED in the fluorescence sensors. This item is a standard component of the [[O2k-Fluorescence LED2-Module]].

{{MitoPedia without banner ...zed cells. To overcome oxygen diffusion limitation in permeabilized fibers and homogenates, an elevated oxygen regime is applied, requiring instrumental b

| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]] - » [[Komlodi Timea]]« and » [[Doerrier Carolina]]«

{{MitoPedia ...b>. Upon accumulation of the dye it exhibits a red shift in its absorption and fluorescence emission spectrum. The fluorescence intensity is quenched when

...-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]] - communicated by »[[Komlodi Timea]]« and [[Iglesias-Gonzalez Javier]]«

{{MitoPedia ...nhibitor of [[NADH electron transfer-pathway state |N-linked respiration]] and of the [[phosphorylation system]].

{{MitoPedia ...up>2+</sup> and ATP-Mg²⁺ and initial concentrations of ADP, ATP and Mg²⁺, the change in ATP concentration in the medium is calculate

{{MitoPedia without banner ...n flux]], and (4) [[flux smoothing]], selection of the [[scaling factor]], and stoichiometric normalization using a stoichiometric coefficient.

| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]] It needs to be determined for each cell type and digitonin batch. The SUIT protocol SUIT-010 was designed to evaluate the op

{{MitoPedia ...or mitochondrial preparations. The reaction of H₂O₂ and AmR is catalyzed by [[horseradish peroxidase]] to produce the red fluoresce

...-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]] ...ab 8 software is designed for 64-bit versions of Windows operating systems and does not run on MAC devices.

| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]] ...chondrial electron transfer system transfers electrons from dehydrogenases and respiratory complexes downstream to Complex III. <br>

....jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] |'''Antioxidant supplementation: Effects of MitoQ and CoQ10 on ROS production and mitochondrial function'''<br>

...ight KJ, Thomas MM, Romestaing C, Hepple RT (2011) Mitochondrial structure and function are disrupted by standard isolation methods. PLoS One 6:e18317. ...function in tissue preparations where mitochondrial structure is preserved and all mitochondria are represented.