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Property "Has-20abstract" (as page type) with input value "-5B-5BImage:MITOEAGLE-2Dlogo.jpg-7Cleft-7C100px-7Clink=http:-2F-2Fwww.mitoglobal.org-2Findex.php-2FMITOEAGLE-7CCOST-20Action-20MitoEAGLE-5D-5D-0AZinc-20is-20an-20essential-20element-20in-20human-20body-20regulating-20many-20physiological-20processes.-20It-20is-20involved-20in-20DNA-20and-20protein-20synthesis,-20apoptosis-20and-20cardiac-20contractility.-20We-20hypothesized-20that-20Zn-3Csup-3E2+-3C-2Fsup-3E-20transporter-20ZIP7-20Zip7-20localize-20not-20only-20into-20SER-20but-20also-20mitochondria-20affect-20the-20sarco(endo)plasmic-20reticulum-20S(E)R-2Dmitochondria-20coupling,-20and-20thereby-20contribute-20to-20cellular-20Zn-3Csup-3E2+-3C-2Fsup-3E-2Dmuffling-20between-20S(E)R-2Dmitochondria-20in-20hyperglycemic-20H9c2-20cells.-20We-20found-20that-20ZIP7-20is-20highly-20expressing-20in-20cardiomyocytes-20and-20H9c2-20cells.-20We-20first-20observed-20subcellular-20localization-20of-20ZIP7-20in-20mitochondria-20by-20using-20fluorescence-20technique-20or-20biochemical-20analysis-20in-20H9c2-20cells.-20We-20incubated-20the-20H9c2-20cells-20with-2025mM-20glucose-20for-2024h-20(HG).-20By-20using-20recombinant-2Dtargeted-20Förster-20resonance-20energy-20transfer-20sensors,-20we-20showed-20that-20HG-20induced-20a-20marked-20redistribution-20of-20cellular-20free-20Zn-3Csup-3E2+-3C-2Fsup-3E,-20increasing-20cytosolic-20and-20mitochondrial-20free-20Zn-3Csup-3E2+-3C-2Fsup-3E-20and-20lowering-20free-20Zn-3Csup-3E2+-3C-2Fsup-3E-20in-20the-20S(E)R.-20On-20the-20other-20hand,-20HG-20caused-20significant-20increase-20of-20ZIP7-20protein-20level-20both-20in-20S(E)R-20and-20mitochondria.-20Deletion-20of-20ZIP7-20prevented-20changes-20of-20S(E)R-20and-20mitochondrial-20and-20intracellular-20free-20Zn-3Csup-3E2+-3C-2Fsup-3E-20in-20HG-20group.-20Determine-20the-20role-20of-20ZIP7-20on-20sarco(endo)plasmic-20reticulum-2Dmitochondria-20coupling-20and-20apoptosis,-20we-20measured-20mitofusin-2Dprotein-20Mfn-2D1-2F2,-20a-20mitochondrial-20ﬁssion-20protein,-20Fis-2D1,-20which-20is-20also-20a-20bridge-20proteins-20between-20mitochondria-2DS(E)R-20interface,-20and-20another-20bridge-20proteins-20between-20mitochondria-2DS(E)R-20interface,-20Bap31-20and-20apoptosis-20markers-20BCL2-20and-20Bax-20in-20H9c2-20cells.-20We-20found-20the-20expression-20levels-20of-20Mfn-2D1-2F2-20and-20Fis-2D1-20in-20ZIP7-20included-20cells-20were-20markedly-20high-20while-20the-20Bap31-20expression-20level-20was-20dramatically-20decreased-20in-20HG-2DH9c2-20cells.-20ZIP7-20silenced-20cells-20slightly-20prevented-20these-20changes.-20Overall,-20this-20study-20provides-20an-20important-20description-20about-20the-20role-20of-20ZIP7,-20localized-20to-20both-20mitochondria-20and-20S(E)R-20and-20contribute-20to-20cellular-20Zn-3Csup-3E2+-3C-2Fsup-3E-2Dmuffling-20between-20cellular-2Dcompartments-20in-20HG-20or-20hypertrophic-20cardiomyocytes-20via-20affecting-20S(E)R-2Dmitochondria-20coupling.-20Any-20alteration-20in-20this-20axis-20and-2For-20cellular-20-5BZn-3Csup-3E2+-3C-2Fsup-3E-5D-20may-20provide-20new-20insight-20for-20prevention-2Ftherapy-20of-20HF-20in-20diabetes-20and-2For-20hypertrophy." contains invalid characters or is incomplete and therefore can cause unexpected results during a query or annotation process.

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