Succinate: Difference between revisions
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|abbr=S | |abbr=S | ||
|description=Succinate<sup>2-</sup> is formed in the [[TCA cycle]], and is a substrate of [[CII]], reacting to fumarate and feeding electrons into the [[Q-junction]] through flavin adenine dinucleotide (FADH2). Succinate supports electron flux exclusively through Complex II via FADH2. | |description=Succinate<sup>2-</sup> is formed in the [[TCA cycle]], and is a substrate of [[CII]], reacting to fumarate and feeding electrons into the [[Q-junction]] through flavin adenine dinucleotide (FADH2). Succinate supports electron flux exclusively through Complex II via FADH2. | ||
|info=MiPNet11.09, MiPNet12.11 | |info=[[MiPNet11.09]], [[MiPNet12.11]] | ||
|type=Respiration | |type=Respiration | ||
}} | }} | ||
{{Labeling | {{Labeling | ||
|discipline=Mitochondrial Physiology | |discipline=Mitochondrial Physiology | ||
|enzymes=Complex II; Succinate Dehydrogenase | |||
|topics=Substrate; Glucose; TCA Cycle | |topics=Substrate; Glucose; TCA Cycle | ||
| | |instruments=Chemicals; Media | ||
|type=Respiration | |||
}} | }} | ||
==HRR== | |||
S: Succinate disodium salt, hexahydrate, C4H4O4Na2.6H2O; FW=270.1. | |||
Stock solution: 1.000 mM (1 M; 2.701 g/10 ml H2O); adjust pH to 7.0 with 37% HCl. Divide into 0.5 ml portions. Store frozen at -20 °C. Sigma, S 2378 (100 g) RT. | |||
Final conc. in 2 ml O2k-chamber: 10 mM; titrate 20 µl using the 50 µl syringe. | |||
Revision as of 19:41, 26 October 2010
- high-resolution terminology - matching measurements at high-resolution
Succinate
Description
Succinate2- is formed in the TCA cycle, and is a substrate of CII, reacting to fumarate and feeding electrons into the Q-junction through flavin adenine dinucleotide (FADH2). Succinate supports electron flux exclusively through Complex II via FADH2.
Abbreviation: S
Reference: MiPNet11.09, MiPNet12.11
Labels:
Enzyme: Complex II; Succinate Dehydrogenase"Complex II; Succinate Dehydrogenase" is not in the list (Adenine nucleotide translocase, Complex I, Complex II;succinate dehydrogenase, Complex III, Complex IV;cytochrome c oxidase, Complex V;ATP synthase, Inner mt-membrane transporter, Marker enzyme, Supercomplex, TCA cycle and matrix dehydrogenases, ...) of allowed values for the "Enzyme" property. Regulation: Substrate; Glucose; TCA Cycle"Substrate; Glucose; TCA Cycle" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property.
HRR: Chemicals; Media"Chemicals; Media" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.
HRR
S: Succinate disodium salt, hexahydrate, C4H4O4Na2.6H2O; FW=270.1.
Stock solution: 1.000 mM (1 M; 2.701 g/10 ml H2O); adjust pH to 7.0 with 37% HCl. Divide into 0.5 ml portions. Store frozen at -20 °C. Sigma, S 2378 (100 g) RT.
Final conc. in 2 ml O2k-chamber: 10 mM; titrate 20 µl using the 50 µl syringe.
