Difference between revisions of "TMRM"
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|abbr=TMRM | |abbr=TMRM | ||
|description='''TMRM''' ( tetramethylrhodamine methyl ester) is an [[extrinsic fluorophores|extrinsic fluorophore]] and can be used as a probe to determine changes in [[Mitochondrial_membrane_potential|mitochondrial membrane potential]]. TMRM is a lipophilic cations that is accumulated by mitochondria in proportion to Ξ''Ο''<sub>mt</sub>. Upon accumulation of the dye it exhibits a red shift in its absorption and fluorescence emission spectrum. The fluorescence intensity is quenched when the dye is accumulated by mitochondria. | |description='''TMRM''' ( tetramethylrhodamine methyl ester) is an [[extrinsic fluorophores|extrinsic fluorophore]] and can be used as a probe to determine changes in [[Mitochondrial_membrane_potential|mitochondrial membrane potential]]. TMRM is a lipophilic cations that is accumulated by mitochondria in proportion to Ξ''Ο''<sub>mt</sub>. Upon accumulation of the dye it exhibits a red shift in its absorption and fluorescence emission spectrum. The fluorescence intensity is quenched when the dye is accumulated by mitochondria. | ||
|info=[[Ehrenberg 1988 Biophys J]] | |||
}} | }} | ||
{{MitoPedia methods | {{MitoPedia methods |
Revision as of 10:09, 21 March 2014
Description
TMRM ( tetramethylrhodamine methyl ester) is an extrinsic fluorophore and can be used as a probe to determine changes in mitochondrial membrane potential. TMRM is a lipophilic cations that is accumulated by mitochondria in proportion to ΞΟmt. Upon accumulation of the dye it exhibits a red shift in its absorption and fluorescence emission spectrum. The fluorescence intensity is quenched when the dye is accumulated by mitochondria.
Abbreviation: TMRM
Reference: Ehrenberg 1988 Biophys J
MitoPedia methods:
Fluorometry
The TMRM method can be used with the O2k-Fluorescence LED2-Module. For an example of the use of TMRM with mouse liver mitochondria see the discussion page of this entry