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| {{Technical support}}
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| Biological contamination is caused by the growth of biological material (originating from samples or otherwise). It should not be confused with other kinds of chamber contamination mentioned in [[Cleaning the glass chamber]].
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| Biological contamination of the chamber is typically characterized by a high O2 flux with a closed chamber near air saturation. It is detected by an [[O2 sensor test]], an [[instrumental background test]] or by observing the flux at closed chamber for a short time routinely before any experiment. In the latter two cases the high flux contamination may be caused by biological contamination of the chamber itself or by a contaminated medium. This can be tested by observing the flux in pure water.
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| The ideal remedial agent is 70% ethanol with 30% water (not 100% ethanol). Make sure the 70% ethanol does not contain any additives. E.g. 70% ethanol used in hospital settings may contain antiseptics (information provided by [[Garcia-Roves PM]]).
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| Prevention of biological contamination of the chamber is primarily by storage under 70% ethanol. If repeated washing with 70% Ethanol does not remove an existing biological contamination, the glass chamber has to be cleaned as described for protein contamination under [[Cleaning the glass chamber]] by removing the glass chamber from the instrument and using a strong acid.
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| {{#set: Technical service =Chamber}}
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| [[Category:Technical service]]
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