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| {{Technical service}}
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| Biological contamination is caused by the growing of biological material (originating from samples or otherwise). It should not be confused with other kinds of chamber contamination mentioned in [[Cleaning the glass chamber]].
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| Biological contamination of the chamber is typically detected by a high O2 flux at closed chamber near air saturation. It is detected by a [[sensor test]], an [[instrumental background test]] or by observing the flux at closed chamber for a short time routinely before any experiment. In the later two cases the high flux contamination may be caused by biological contamination of the chamber itself or by a contaminated medium. This can be tested by observing the flux in pure water.
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| The ideal counter agent is 70% Ethanol with 30% water (NOT 100% Ethanol). Make sure the 70% ethanol does not contain any additives. E.g. 70% ethanol used in hospital settings may contain antiseptics (information provided by [[Garcia-Roves PM]]).
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| Prevention of biological contamination of the chamber is primarily by storage under 70% ethanol. If repeated washing with 70% Ethanol does nor remove an already present biological contamination, the glass chamber has to be cleaned as described for Protein contamination under [[Cleaning the glass chamber]] by removing the glass chamber form the instrument and using a strong acid.
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| {{#set: Technical service =Chamber}}
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| [[Category:Technical service]]
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| __SHOWFACTBOX__
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