Difference between revisions of "Talk:O2k-Videosupport"

Revision as of 18:27, 12 February 2018

Last update: 2018-02-12

Videoclip for pfi

02:00 --> When BIOPS is added to the wells (plate): add the text: all wells 2 mL BIOPS. The text will stay until: 02:10.
02:18 --> We add 1 mL of BIOPS into the eppendorf tube. Show the pippete with 1 mL (instead of the 2 mL pippete). We add 1 mL of BIOPS to the Petridish as well.
03:00 --> Add text for the saponin concentration --> 5 mg/mL in BIOPS
03:37 --> Add text with the information --> 20 µL saponin in 2 mL BIOPS
03:42 --> Add text with the information --> BIOPS (it will be in the first line of the plate); BIOPS+saponin (in the second line); BIOPS (in the last line)
04:41 --> Incorporate images from the binocular. Johannes, maybe is possible to do any animation (with the panel showing all the steps)?
06:49 --> Add the text: Aluminum cup on the petri dish
07:50 --> Add the text: Blotting during 40 seconds
08:13 --> Add to the video (if Johannes took it, I think he took it. Checking if the forceps are dry after taking the wet weight) --> and add the text: check the forceps
08:16 --> Add the text info?: take wet weight
~08:39-08:40 --> When we take the forceps, we can see something.
~08:56-08:59 --> Add the text: Add O₂

Clips for pfi

1. Materials (00:00-01:30)

2. BIOPS and saponin (01:30-03:55)

3. Muscle fibre preparation: Mechanical Preparation (03:55-04:57)

4. Muscle fibre preparation: Chemical Permeabilization (04:57-06:33)

5. Wet Weight (06:34-08:17)

6. To the O2k (08:18-09:15)

Videoclip for Shredder

~00:19 --> Add text? Calculate volume needed for your thom? (here, pipette adding 5 mL, show pipette 5 mL (00:41)).
00:41 --> Not 5 mL here, show pippete with 500 µL.
~02:06 --> Text? Cut in small pieces?
~02:21 --> Text? Distribute the sample in the surface?
~04:02 --> 5 seconds instead of 10 seconds (in the position 2)
~07:33--> Not all the volume is take it. Does Johannes has another video?
Last part of the video is in the pfi video.

Clips for Shredder

1. Materials (00:00-01:30)

2. Tissue preparation (01:30-03:02)

3. Homogenization (~03:02-04:27)

4. Retrieval (~04:27-to the end)

5. To the O2k (from 09:12 in the pfi video)

@@ Line 1: / Line 1: @@
+Last update: 2018-02-12
 == Videoclip for pfi ==
-::::# When BIOPS and saponin is added to the wells (plate), maybe we could add a text: BIOPS; BIOPS+saponin; BIOPS
+::::# 02:00 --> When BIOPS is added to the wells (plate): add the text: all wells 2 mL BIOPS. The text will stay until: 02:10.
-::::# 02:18 --> we add 1 mL of BIOPS into the eppendorf tube. Show the pippete with 1 mL. We add 1 mL of BIOPS to the Petridish as well.
+::::# 02:18 --> We add 1 mL of BIOPS into the eppendorf tube. Show the pippete with 1 mL (instead of the 2 mL pippete). We add 1 mL of BIOPS to the Petridish as well.
-::::# ~03:03 --> any text for the saponin concentration? --> 5 mg/mL
+::::# 03:00 --> Add text for the saponin concentration --> 5 mg/mL in BIOPS
-::::# ~03:44 --> add text with the information? --> 20 µL saponin in 2 mL BIOPS
+::::# 03:37 --> Add text with the information --> 20 µL saponin in 2 mL BIOPS
-::::# ~03:50 --> add text with the information into the plate? --> BIOPS (2 mL?); BIOPS+saponin (50 µg saponin/mL); BIOPS (2 mL?)
+::::# 03:42 --> Add text with the information --> BIOPS (it will be in the first line of the plate); BIOPS+saponin (in the second line); BIOPS (in the last line)
-::::# Incorporate images from the fiberts (from the binocular)?:
+::::# 04:41 --> Incorporate images from the binocular. Johannes, maybe is possible to do any animation (with the panel showing all the steps)?
-::::::* ~04:30 --> "clean the tissue" picture? or all together (panel)?
+::::# 06:49 --> Add the text: Aluminum cup on the petri dish
-::::::* ~04:33 --> Divide the tissue in small pieces.
+::::# 07:50 --> Add the text: Blotting during 40 seconds
-::::::* I miss in the video the separation of the pfi.
+::::# 08:13 --> Add to the video (if Johannes took it, I think he took it. Checking if the forceps are dry after taking the wet weight) --> and add the text: check the forceps
-::::::* ~04:44 --> panel showing all tghe steps?
+::::# 08:16 --> Add the text info?: take wet weight
-::::# ~07:47 --> Blotting during 40 sec (add text).
-::::# ~08:13 --> Add video (if Johannes took it, I think he took it) --> checking if the forceps are wet or dry after the wet weight.
-::::# ~08:16 --> Text info?: take wet weight.
 ::::# ~08:39-08:40 --> When we take the forceps, we can see something.
-::::# ~08:56-08:59 --> Text? Needle into the gas phase?; text? Add O2.
+::::# ~08:56-08:59 --> Add the text: Add O<sub>2</sub>
 === Clips for pfi ===
-::::# Material preparation (00:00-03:55)
+::::1. Materials (00:00-01:30)
-::::::* Part I (00:00-01:30)
+::::2. BIOPS and saponin (01:30-03:55)
-::::::* Part II (01:30-03:55)
+::::3. Muscle fibre preparation: Mechanical Preparation (03:55-04:57)
-::::# Sample preparation (03:55-03:55). Together or different clips?
+::::4. Muscle fibre preparation: Chemical Permeabilization (04:57-06:33)
-::::::* Mechanical Preparation of Permeabilized Fibres (03:55-04:57)
+::::5. Wet Weight (06:34-08:17)
-::::::* Chemical Permeabilization (04:57-06:33)
+::::6. To the O2k (08:18-09:15)
-::::# Wet Weight of Muscle Fibres (06:34-08:17)
-::::# Transferring the sample into the O2k-Chamber/Add O2 (08:18-09:15)
@@ Line 39: / Line 36: @@
 === Clips for Shredder ===
-::::# Material preparation (00:00-01:30). Include sample preparation (01:30-03:02 or different clips?
+::::1. Materials (00:00-01:30)
-::::# Shredder homogenization (~03:02-04:27)
+::::2. Tissue preparation (01:30-03:02)
-::::# Sample collection (~04:27-to the end)
+::::3. Homogenization (~03:02-04:27)
-::::# Add sample into the O2k (07:47-is in the video for pfi, from 09:12).
+::::4. Retrieval (~04:27-to the end)
+::::5. To the O2k (from 09:12 in the pfi video)