Talk:O2k-Workshops in O2k-Network Labs

Preparation for a locally organized O2k-Workshop

Organization and programme

1A. Participants

1. How many participants are expected?
2. Is it a homogenous group of either (a) experienced users or (b) unexperienced interested participants? Or is the group mixed?
3. Is a list of participants available, indicating participants who are O2k-Users?
4. Are name tags prepared?

1B. O2k-Demo

1. For experienced users, a hands-on session on instrumental assembly and service is important, and a corresponding session should be organized in small groups.
2. For an unexperienced group, an O2k-Demo oriented workshop is perferrably conducted in a seminar room.
3. Is a demo experiment to be prepared with biological samples provided locally (tissue, cultured cells)? Usually we provide freeze-dried yeast as biological sample for a demo experiment of respiration in intact cells (see below). The demo experiment can be run in a seminar room.
4. Is a projector available for PowerPoint presentations and for connecting the laptop connected with DatLab to the O2k?

Instruments and accessories

2A. Which and how many OROBOROS Oxygraph-2k are locally available?

1. Specify O2k-Series number(s) of demo instrument.
2. Are O2k-Users invited to bring their instrument for practice and guided service? Do they bring their laptop for DatLab?

2B. Which accessories and consumables are locally available?

1. OroboPOS-Service Kit (necessary) including OroboPOS-Membranes and OroboPOS-Electrolyte Powder (or freshly prepared electrolyte).
2. ISS-Integrated Suction System (necessary).
3. Titration Set (some Hamilton syringes are specifically required for manual titrations).
4. HRR-Dissection Set (required if permeabilized tissue preparation is to be demonstrated).
5. PBI-Shredder HRR-Set (required if tissue homogenate should be prepared).
6. Microbalance-Set (required if tissue mass, particularly wet weight, should be determined).

2C. Which O2k-Modules are locally available?

1. TIP2k-Module (optional)
2. O2k-Fluorescence LED2-Module (recommended)
3. O2k-TPP+ and Ca2+ ISE-Module (only for special ISE workshop)
4. O2k-pH ISE-Module (only for special O2k-MultiSensor workshop)
5. O2k-NO Amp-Module and separate NO sensor (only for special O2k-MultiSensor workshop)