Talk:Rogers 2011 PLoS One
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Comments by Erich Gnaiger
This paper describes application of a strongly advertised multi-well system (XF, 'Seahorse Bioscience') for respirometry with isolated mitochondria. Whereas multi-well systems do provide high throughput, it must be critically assessed if this is equivalent to high output of useful data and information. Based on expertise in high-resolution respirometry and mitochondrial physiology, the paper by Rogers et al (2011) is discussed.
Conflicts of interest:
- Rogers GW and Ferrick DA are employees of Seahorse Bioscience (at the time of publication).
- Gnaiger E is founder and director of OROBOROS INSTRUMENTS.
Instrumental specifications of the XF multi-well system are missing
Comparison with low-resolution instruments
Low-resolution instruments (compare HRR) with Clark-type polarographic oxygen sensors were used for validation of data obtained with the XF.
