Difference between revisions of "Template:AmR assay"

Latest revision as of 15:04, 12 February 2019

Step	State	Pathway	Q-junction	Comment - Events (E) and Marks (M)
0DTPA				DTPA is an iron chelator, which decreases the chemical fluorescence background created by the Amplex UltraRed assay. Administration of DTPA into the O2k-chamber is recommended before all other chemicals because the iron chelation capacity of the compound is time-dependent (approx. 10-15 min). However, the experiments can be carried out in the absence of DTPA.
0SOD				SOD or superoxide dismutase converts the anion superoxide released by the mitochondria into H₂O₂, making it accessible to the Amplex UltraRed assay.
0HRP				HRP or horseradish peroxidase catalyses the conversion of Amplex UltraRed and H₂O₂ towards the fluorescent resorufin.
0AmR				AmR or Amplex UltraRed forms resorufin with H₂O₂ catalysed by horseradish peroxidase HRP.

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-[[Image:SUIT-MitoFit.png|right|190px|link=http://www.bioblast.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]]
-== Steps and respiratory states ==
 {| class="wikitable" border="1"
 {{Template:SUIT header}}
-|- 0DTPA
+|-
-| [[DTPA]]
+| 0[[DTPA]]
+|
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-|
+*{{Template:DTPA}}
- {{Template:DTPA}}
-|- 0SOD
+|-
-| [[Superoxide dismutase|SOD]]
+| 0[[Superoxide dismutase|SOD]]
+|
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+*{{Template:SOD}}
+|-
+| 0[[Horseradish peroxidase|HRP]]
+|
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- {{Template:SOD}}
-|- 0HRP
-| [[Horseradish peroxidase|HRP]]
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-|
+*{{Template:HRP}}
-|
+|-
- {{Template:HRP}}
+| 0[[Amplex UltraRed|AmR]]
-|- 0AmR
+|
-| [[Amplex UltraRed|AmR]]
-|
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- {{Template: AmR}}
+*{{Template: AmR}}