Difference between revisions of "Template:Current week ecosystem agenda"

Revision as of 12:04, 29 April 2024

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-» A future for Ukraine is the future of its science

2024 Week 18

Apr-29 Mo

How can I update DatLab 8 to the latest version?

If your computer with DatLab 8 is connected to the internet, go to “Check for updates” in the Help menu of DatLab 8, and follow the simple installation instructions.
- »Updating - DatLab«, »DatLab 8«
- communicated by »Leo Elettra« and »Baglivo Eleonora«

Apr-30 Tu

May-01 We

May-02 Th

"In respirometric studies on blood cells, the purity of cell preparation should always be emphasized and documented."

- »MiPNet21.17 BloodCellsIsolation«
- communicated by »Timon-Gomez Alba« and »Baglivo Eleonora«

May-03 Fr

Next Week

2024 Week 19

May-06 Mo

What is the effective concentration of digitonin for complete plasma membrane permeabilization?

It needs to be determined for each cell type and digitonin batch. The SUIT protocol SUIT-010 was designed to evaluate the optimum digitonin concentration to permeabilize the plasma membrane.
- »Digitonin«, »SUIT-010«
- communicated by »Schmitt Sabine« and »Timon-Gomez Alba«

May-07 Tu

May-08 We

May-09 Th

May-10 Fr

@@ Line 1: / Line 1: @@
+{{Template:Science for Ukraine}}
-Week 27
+Week 18
-* '''Jun-29 Mo'''
+* '''Apr-29 Mo'''
 :{|
-| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]]
+| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
-|'''Is the noise seen in the slope of the oxygen signal (O2 slope neg.) within recommended values?'''<br>
+|'''How can I update DatLab 8 to the latest version?'''<br>
-: An 'O2 slope neg.' within the range of ± 4 pmol∙s<sup>−1</sup>∙mL<sup>−1</sup> is acceptable<br>
+If your computer with DatLab 8 is connected to the internet, go to “Check for updates” in the Help menu of DatLab 8, and follow the simple installation instructions. <br>
-- » [[Oxygen_calibration_-_DatLab#Instrumental_DL-Protocols| Air and zero calibration]]«
+- »'''[[Updating - DatLab]]'''«, »'''[https://www.oroboros.at/index.php/product/datlab/ DatLab 8]'''«<br>
+- communicated by »[[Leo Elettra]]« and »[[Baglivo Eleonora]]«
 |}
 ----
-* '''Jun-30 Tu'''
+* '''Apr-30 Tu'''
+----
+* '''May-01 We'''
+----
+* '''May-02 Th'''
 :{|
-| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
+| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
-|'''The antidiabetic drug empagliflozin protects cardiac mitochondria against high-fat diet-induced dysfunction in mice'''<br>
+|''' "In respirometric studies on blood cells, the purity of cell preparation should always be emphasized and documented." '''<br>
-:from the O2k-Network »[[LV Riga Makrecka-Kuka M]]«
+- »'''[[MiPNet21.17 BloodCellsIsolation]]'''«<br>
-*Makrecka-Kuka M, Korzh S, Videja M, Vilks K, Cirule H, Kuka J, Dambrova M, Liepinsh E (2020) Empagliflozin protects cardiac mitochondrial fatty acid metabolism in a mouse model of diet-induced lipid overload. Cardiovasc Drugs Ther [Epub ahead of print]. [[Makrecka-Kuka 2020 Cardiovasc Drugs Ther|»Bioblast link«]]
+- communicated by »[[Timon-Gomez Alba]]« and »[[Baglivo Eleonora]]«
 |}
 ----
-* '''Jul-01 We'''
+* '''May-03 Fr'''
+'''<big>Next Week</big>'''
+Week 19
-----
+* '''May-06 Mo'''
-* '''Jul-02 Th'''
 :{|
- | width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]
+| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
- | '''Did you know that if [[ET_capacity|ET capacity]] [[File:E.jpg]] is lower than [[OXPHOS_capacity|OXPHOS capacity]] [[File:P.jpg]] ([[ET_capacity#The_important_difference_between_states_P_and_E|''P''>''E'']]) in [[Living_cells | living cells]] or [[Mitochondrial_preparations | mitochondrial preparations]], then you have encountered an experimental artefact? The possible triggers of this experimental artefact are:''' <br>
+|''' What is the effective concentration of digitonin for complete plasma membrane permeabilization?'''<br>
-- loss of oxidative capacity during the time course of the respirometric assay,<br>
+It needs to be determined for each cell type and digitonin batch. The SUIT protocol SUIT-010 was designed to evaluate the optimum digitonin concentration to permeabilize the plasma membrane. <br>
-- insufficient [[Uncoupler|uncoupler]] concentrations,<br>
+- »'''[[Digitonin]]'''«, »'''[[SUIT-010]]'''« <br>
-- over titration of an uncoupler, which inhibits ET capacity,<br>
+- communicated by »[[Schmitt Sabine]]« and »[[Timon-Gomez Alba]]«
-- inhibition of ET capacity due to the use of high [[Oligomycin|oligomycin]] (inhibitor of the ATP synthase) concentrations employed to measure [[LEAK_respiration|LEAK respiration]] prior ET capacity.
-Information from: Gnaiger Erich ''et al'' ― MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1 »[[BEC_2020.1_doi10.26124bec2020-0001.v1|Bioblast link]]«
 |}
 ----
-* '''Jul-03 Fr'''
+* '''May-07 Tu'''
-:{|
+----
-| width="100" align="center" | [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]]
+* '''May-08 We'''
-|'''Mitochondrial H<sub>2</sub>O<sub>2</sub> flux in cardiac and skeletal muscle preparations is influenced by experimental oxygen concentration''' <br>
+----
-: from the O2k-Network »[[US CO Fort Collins Chicco AJ]]« <br>
+* '''May-09 Th'''
-*           Li Puma LC, Hedges M, Heckman JM, Mathias AB, Engstrom MR, Brown AB, Chicco AJ (2020) Experimental oxygen concentration influences rates of mitochondrial hydrogen peroxide release from cardiac and skeletal muscle preparations. Am J Physiol Regul Integr Comp Physiol 318:R972-80. [[Li Puma 2020 Am J Physiol Regul Integr Comp Physiol|»Bioblast link«]]
+----
-|}
+* '''May-10 Fr'''