Template:Current week ecosystem agenda: Difference between revisions

Revision as of 12:04, 29 April 2024

Oroboros declares solidarity against the Russian aggression-war in Ukraine and against all aggression-wars worldwide. - »More details

-» A future for Ukraine is the future of its science

2024 Week 18

Apr-29 Mo

How can I update DatLab 8 to the latest version?

If your computer with DatLab 8 is connected to the internet, go to “Check for updates” in the Help menu of DatLab 8, and follow the simple installation instructions.
- »Updating - DatLab«, »DatLab 8«
- communicated by »Leo Elettra« and »Baglivo Eleonora«

Apr-30 Tu

May-01 We

May-02 Th

"In respirometric studies on blood cells, the purity of cell preparation should always be emphasized and documented."

- »MiPNet21.17 BloodCellsIsolation«
- communicated by »Timon-Gomez Alba« and »Baglivo Eleonora«

May-03 Fr

Next Week

2024 Week 19

May-06 Mo

What is the effective concentration of digitonin for complete plasma membrane permeabilization?

It needs to be determined for each cell type and digitonin batch. The SUIT protocol SUIT-010 was designed to evaluate the optimum digitonin concentration to permeabilize the plasma membrane.
- »Digitonin«, »SUIT-010«
- communicated by »Schmitt Sabine« and »Timon-Gomez Alba«

May-07 Tu

May-08 We

May-09 Th

May-10 Fr

@@ Line 1: / Line 1: @@
-Week 30
+{{Template:Science for Ukraine}}
-* '''Jul-20 Mo'''
+Week 18
+* '''Apr-29 Mo'''
 :{|
 | width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
-|'''How can you compare respirometric experiments using an internal normalization?'''<br>
+|'''How can I update DatLab 8 to the latest version?'''<br>
-Flux control ratios (''FCR'') are independent of external measurements (''e.g.'' protein determination, citrate synthase activity) and therefore can be used to analyze data when other normalization methods cannot be applied. Learn more about how to use this option to assess qualitative changes of mitochondrial respiratory control with DatLab and the “O2 analysis template DL7.4”.
+If your computer with DatLab 8 is connected to the internet, go to “Check for updates” in the Help menu of DatLab 8, and follow the simple installation instructions. <br>
-<br>
+- »'''[[Updating - DatLab]]'''«, »'''[https://www.oroboros.at/index.php/product/datlab/ DatLab 8]'''«<br>
-- »[[Flux control ratio]]«
+- communicated by »[[Leo Elettra]]« and »[[Baglivo Eleonora]]«
-Read more: Gnaiger Erich ''et al'' ― MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1 »[[BEC_2020.1_doi10.26124bec2020-0001.v1|Bioblast link]]«
 |}
 ----
-* '''Jul-21 Tu'''
+* '''Apr-30 Tu'''
+----
+* '''May-01 We'''
+----
+* '''May-02 Th'''
 :{|
-| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
+| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
-|'''Absence of AMPK in skeletal muscle impacts mitochondrial content and respiration in addition to mitigating glucose intolerance induced by a high-fat diet'''<br>
+|''' "In respirometric studies on blood cells, the purity of cell preparation should always be emphasized and documented." '''<br>
-:from the O2k-Network »[[US TN Nashville Wasserman DH]]« and »[[US MN Minneapolis Hughey C]]«
+- »'''[[MiPNet21.17 BloodCellsIsolation]]'''«<br>
-*Lantier L, Williams AS, Williams IM, Guerin A, Bracy DP, Goelzer M, Foretz M, Viollet B, Hughey CC, Wasserman DH (2020) Reciprocity between skeletal muscle AMPK deletion and insulin action in diet-induced obese mice. Diabetes [Epub ahead of print].  [[Lantier 2020 Diabetes|»Bioblast link«]]
+- communicated by »[[Timon-Gomez Alba]]« and »[[Baglivo Eleonora]]«
 |}
 ----
-* '''Jul-22 We'''
+* '''May-03 Fr'''
+'''<big>Next Week</big>'''
+Week 19
-----
+* '''May-06 Mo'''
-* '''Jul-23 Th'''
 :{|
-| width="110" align="center" | [[File:O2k-Webinars icon.png|left|80px|MitoPedia]]
+| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
-| '''First [[O2k-Webinars|O2k-Webinar]] - O2k-Applications: H<sub>2</sub>O<sub>2</sub> production by [[Komlodi Timea|Dr. Tímea Komlódi]] '''
+|''' What is the effective concentration of digitonin for complete plasma membrane permeabilization?'''<br>
+It needs to be determined for each cell type and digitonin batch. The SUIT protocol SUIT-010 was designed to evaluate the optimum digitonin concentration to permeabilize the plasma membrane. <br>
+- »'''[[Digitonin]]'''«, »'''[[SUIT-010]]'''« <br>
+- communicated by »[[Schmitt Sabine]]« and »[[Timon-Gomez Alba]]«
 |}
 ----
-* '''Jul-24 Fr'''
+* '''May-07 Tu'''
-:{|
+----
-| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]
+* '''May-08 We'''
-| '''Do you know how to assess the integrity of the [[mitochondrial outer membrane]] in [[mitochondrial preparations]]? '''
+----
-It is possible to do so using exogenously added [[cytochrome c|cytochrome ''c'']]: <br>
+* '''May-09 Th'''
-- »'''[[Cytochrome_c_control_factor#Determination_of_cytochrome_c_loss|Cytochrome c control factor]]'''«
-|}
 ----
-* '''Jul-25 Sa'''
+* '''May-10 Fr'''