Template:Current week ecosystem agenda: Difference between revisions

Revision as of 12:04, 29 April 2024

Oroboros declares solidarity against the Russian aggression-war in Ukraine and against all aggression-wars worldwide. - »More details

-» A future for Ukraine is the future of its science

2024 Week 18

Apr-29 Mo

How can I update DatLab 8 to the latest version?

If your computer with DatLab 8 is connected to the internet, go to “Check for updates” in the Help menu of DatLab 8, and follow the simple installation instructions.
- »Updating - DatLab«, »DatLab 8«
- communicated by »Leo Elettra« and »Baglivo Eleonora«

Apr-30 Tu

May-01 We

May-02 Th

"In respirometric studies on blood cells, the purity of cell preparation should always be emphasized and documented."

- »MiPNet21.17 BloodCellsIsolation«
- communicated by »Timon-Gomez Alba« and »Baglivo Eleonora«

May-03 Fr

Next Week

2024 Week 19

May-06 Mo

What is the effective concentration of digitonin for complete plasma membrane permeabilization?

It needs to be determined for each cell type and digitonin batch. The SUIT protocol SUIT-010 was designed to evaluate the optimum digitonin concentration to permeabilize the plasma membrane.
- »Digitonin«, »SUIT-010«
- communicated by »Schmitt Sabine« and »Timon-Gomez Alba«

May-07 Tu

May-08 We

May-09 Th

May-10 Fr

@@ Line 1: / Line 1: @@
+{{Template:Science for Ukraine}}
-Week 48
+Week 18
-* '''Nov-23 Mo'''
+* '''Apr-29 Mo'''
 :{|
-| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Yellow-take care.png|25px|Take care]]
+| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
-|'''Cell culture medium and dithionite titrations – a bad combination for an instrumental O<sub>2</sub> background test?'''<br>
+|'''How can I update DatLab 8 to the latest version?'''<br>
-Cell culture media are not ideal for instrumental O<sub>2</sub> background test when using dithionite titrations because side reactions affecting the O<sub>2</sub> concentration and thus interfering with the test. Instead of cell culture media, use [[MiR05]], [[MiR06]] or a strongly buffered alkaline phosphate solution. <br>
+If your computer with DatLab 8 is connected to the internet, go to “Check for updates” in the Help menu of DatLab 8, and follow the simple installation instructions. <br>
--»[[MiPNet14.06 Instrumental O2 background]]« <br>
+- »'''[[Updating - DatLab]]'''«, »'''[https://www.oroboros.at/index.php/product/datlab/ DatLab 8]'''«<br>
-- communicated by »[[Komlodi Timea]]« and »[[Cecatto Cristiane]]«
+- communicated by »[[Leo Elettra]]« and »[[Baglivo Eleonora]]«
 |}
 ----
-* '''Nov-24 Tu'''
+* '''Apr-30 Tu'''
-:::: [[ISCaM 2020 Virtual Event]]
+----
+* '''May-01 We'''
 ----
-* '''Nov-25 We'''
+* '''May-02 Th'''
 :{|
-| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
+| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
-|'''Eight-week high-fat feeding increases mitochondrial ROS production in white adipocytes but mitochondrial respiration remains unaffected.'''<br>
+|''' "In respirometric studies on blood cells, the purity of cell preparation should always be emphasized and documented." '''<br>
-: from the O2k-Network »[[CA Guelph Holloway GP]]«
+- »'''[[MiPNet21.17 BloodCellsIsolation]]'''«<br>
-*Politis-Barber V, Brunetta HS, Paglialunga S, Petrick HL, Holloway GP (2020) Long-term high-fat feeding exacerbates short-term increases in adipose mitochondrial reactive oxygen species, without impairing mitochondrial respiration. Am J Physiol Endocrinol Metab 319:E376-87. [[Politis-Barber 2020 Am J Physiol Endocrinol Metab|»Bioblast link«]]
+- communicated by »[[Timon-Gomez Alba]]« and »[[Baglivo Eleonora]]«
 |}
 ----
-* '''Nov-26 Th'''
+* '''May-03 Fr'''
-----
+'''<big>Next Week</big>'''
-* '''Nov-27 Fr'''
+Week 19
+* '''May-06 Mo'''
 :{|
-| width="100" align="center" | [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]]
+| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
-|'''''HSP60'' knockdown and its effect on mitochondria’s role in pancreatic ductal adenocarcinoma''' <br>
+|''' What is the effective concentration of digitonin for complete plasma membrane permeabilization?'''<br>
+It needs to be determined for each cell type and digitonin batch. The SUIT protocol SUIT-010 was designed to evaluate the optimum digitonin concentration to permeabilize the plasma membrane. <br>
-* Zhou C, Sun H, Zheng C, Gao J, Fu Q, Hu N, Shao X, Zhou Y, Xiong J, Nie K, Zhou H, Shen L, Fang H, Lyu J (2018) Oncogenic HSP60 regulates mitochondrial oxidative phosphorylation to support Erk1/2 activation during pancreatic cancer cell growth. Cell Death Dis 9:161.  [[Zhou 2018 Cell Death Dis|»Bioblast link«]]
+- »'''[[Digitonin]]'''«, »'''[[SUIT-010]]'''« <br>
+- communicated by »[[Schmitt Sabine]]« and »[[Timon-Gomez Alba]]«
 |}
+----
+* '''May-07 Tu'''
+----
+* '''May-08 We'''
+----
+* '''May-09 Th'''
+----
+* '''May-10 Fr'''