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2021 Week 18
{{Template:Science for Ukraine}}


*'''May-03 Mo'''  
2024 Week 18
 
* '''Apr-29 Mo'''
:{|
:{|
| width="100" align="center" |[[File:MitoFit Preprints pdf.png|100px|link=https://www.mitofit.org/index.php/Krako_Jakovljevic_2021_MitoFit_PD |MitoFit pdf]]
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
|'''Exploring photosynthesis and dark respiration in the microalgae ''Chlamydomonas reinhardtii<span dir="ltr"></span>'''''<br>
|'''How can I update DatLab 8 to the latest version?'''<br>
Measurement of oxygen dependence with High-Resolution PhotoRespirometry:<br>
If your computer with DatLab 8 is connected to the internet, go to “Check for updates” in the Help menu of DatLab 8, and follow the simple installation instructions. <br>
* Went N, Di Marcello M, Gnaiger E (2021) Oxygen dependence of photosynthesis and light-enhanced dark respiration studied by High-Resolution PhotoRespirometry. MitoFit Prep 2021.5.  [[Went 2021 MitoFit PB| doi:10.26124/mitofit:2021-0005]]
- »'''[[Updating - DatLab]]'''«, »'''[https://www.oroboros.at/index.php/product/datlab/ DatLab 8]'''«<br>
- communicated by »[[Leo Elettra]]« and »[[Baglivo Eleonora]]«
|}
|}
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*'''May-04 Tu'''  
* '''Apr-30 Tu'''
----
* '''May-01 We'''   
----
* '''May-02 Th'''  
:{|
:{|
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
|''' Do you know how to apply the air and zero calibration values in DatLab?'''<br>
|''' "In respirometric studies on blood cells, the purity of cell preparation should always be emphasized and documented." '''<br>
This important quality control step ensures accurate measurement of respiration.<br>
- »'''[[MiPNet21.17 BloodCellsIsolation]]'''«<br>
For details on how to apply air and zero calibration in DatLab, see:<br>
- communicated by »[[Timon-Gomez Alba]]« and »[[Baglivo Eleonora]]«
»'''[[Oxygen calibration - DatLab]]'''« and »'''[[Oxygen_calibration_-_DatLab#Troubleshooting|Troubleshooting]]'''« <br>
- communicated by »[[Komlodi Timea]]« and »[[Cecatto Cristiane]]«
|}
|}
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*'''May-05 We'''  
* '''May-03 Fr'''
 
'''<big>Next Week</big>'''
2024 Week 19
 
* '''May-06 Mo'''
:{|
:{|
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
|'''Succinate: a key regulator of mitochondrial function and HIF-1α upregulation in prostate cancer''' <br>
|''' What is the effective concentration of digitonin for complete plasma membrane permeabilization?'''<br>
:from the O2k-Network »[[AT Innsbruck Oroboros]]«, »[[AT Innsbruck Gnaiger E]]«  
It needs to be determined for each cell type and digitonin batch. The SUIT protocol SUIT-010 was designed to evaluate the optimum digitonin concentration to permeabilize the plasma membrane. <br>
*Weber A, Klocker H, Oberacher H, Gnaiger E, Neuwirt H, Sampson N, Eder IE (2018) Succinate accumulation is associated with a shift of mitochondrial respiratory control and HIF-1α upregulation in PTEN negative prostate cancer cells. Int J Mol Sci 19:2129. [[Weber 2018 Int J Mol Sci|»Bioblast link«]]
- »'''[[Digitonin]]'''«, »'''[[SUIT-010]]'''« <br>
- communicated by »[[Schmitt Sabine]]« and »[[Timon-Gomez Alba]]«
|}
|}
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*'''May-06 Th'''  
* '''May-07 Tu'''
 
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* '''May-08 We'''
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* '''May-09 Th'''  
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*'''May-07 Fr'''  
* '''May-10 Fr'''
:{|
| width="100" align="center" | [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]]
|'''Cell-permeable succinate can restore statin-inhibited mitochondrial respiration ''' <br>
:from the O2k-Network »[[RO Timisoara Muntean DM]]« and »[[SE Lund Elmer E]]«
 
* Avram VF, Chamkha I, Åsander-Frostner E, Ehinger JK, Timar RZ, Hansson MJ, Muntean DM, Elmér E (2021) Cell-permeable succinate rescues mitochondrial respiration in cellular models of statin toxicity. Int J Mol Sci 22:424. [[Avram 2021 Int J Mol Sci|»Bioblast link«]]
|}

Revision as of 12:04, 29 April 2024

Gentle Science
Oroboros declares solidarity against the Russian aggression-war in Ukraine and against all aggression-wars worldwide. -  »More details
A future for Ukraine is the future of its science
2024 Week 18
  • Apr-29 Mo
O2k-Open SupportInfo: improve How can I update DatLab 8 to the latest version?

If your computer with DatLab 8 is connected to the internet, go to “Check for updates” in the Help menu of DatLab 8, and follow the simple installation instructions.
- »Updating - DatLab«, »DatLab 8«
- communicated by »Leo Elettra« and »Baglivo Eleonora«


  • Apr-30 Tu

  • May-01 We

  • May-02 Th
O2k-Open SupportInfo: improve "In respirometric studies on blood cells, the purity of cell preparation should always be emphasized and documented."

- »MiPNet21.17 BloodCellsIsolation«
- communicated by »Timon-Gomez Alba« and »Baglivo Eleonora«


  • May-03 Fr

Next Week

2024 Week 19
  • May-06 Mo
MitoPedia
What is the effective concentration of digitonin for complete plasma membrane permeabilization?

It needs to be determined for each cell type and digitonin batch. The SUIT protocol SUIT-010 was designed to evaluate the optimum digitonin concentration to permeabilize the plasma membrane.
- »Digitonin«, »SUIT-010« 
- communicated by »Schmitt Sabine« and »Timon-Gomez Alba« 


  • May-07 Tu

  • May-08 We

  • May-09 Th

  • May-10 Fr
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