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Difference between revisions of "Template:Current week ecosystem agenda"

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2022 Week 31
{{Template:Science for Ukraine}}
{{Template:Science for Ukraine}}
*'''Aug-01 Mo''' [[Image:O2k-Workshops.png|60px|link=MiPNet27.03 IOC154 Innsbruck AT]]  <big>'''[[MiPNet27.03 IOC154 Innsbruck AT|154th Oroboros O2k-Coaching Days - Advanced and NextGen-O2k]]'''</big> Aug 1-5


2024 Week 18
* '''Apr-29 Mo'''
:{|
:{|
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
|''' O2k-checklist: Get started with an O2k-experiment''' <br>
|'''How can I update DatLab 8 to the latest version?'''<br>
The O2k-checklist provides an overview of all essential steps for starting an O2k-experiment.
If your computer with DatLab 8 is connected to the internet, go to “Check for updates” in the Help menu of DatLab 8, and follow the simple installation instructions. <br>
<br>
- »'''[[Updating - DatLab]]'''«, »'''[https://www.oroboros.at/index.php/product/datlab/ DatLab 8]'''«<br>
- » '''[[MiPNet20.04_O2k-checklist]]'''« <br>
- communicated by »[[Leo Elettra]]« and »[[Baglivo Eleonora]]«
- » communicated by »[[Cecatto Cristiane]]« and »[[Schmitt Sabine]]«
|}
|}
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*'''Aug-02 Tu''' [[Image:O2k-Workshops.png|60px|link=MiPNet27.03 IOC154 Innsbruck AT]]  <big>'''[[MiPNet27.03 IOC154 Innsbruck AT|154th Oroboros O2k-Coaching Days - Advanced and NextGen-O2k]]'''</big> Aug 1-5
* '''Apr-30 Tu'''  
 
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* '''May-01 We'''  
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* '''May-02 Th'''
:{|
:{|
| width="110" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]]  
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
|''' What are the substrate-uncoupler-inhibitor titration reference protocols?'''<br>
|''' "In respirometric studies on blood cells, the purity of cell preparation should always be emphasized and documented." '''<br>
These protocols provide a common baseline for comparison of mitochondrial respiratory control in a large variety of species, tissues and cell types, mt-preparations and laboratories.
- »'''[[MiPNet21.17 BloodCellsIsolation]]'''«<br>
<br>
- communicated by »[[Timon-Gomez Alba]]« and »[[Baglivo Eleonora]]«
- »''' [[SUIT reference protocol]]'''«
 
- communicated by »[[Cardoso Luiza HD]]« and »[[Tindle-Solomon Lisa]]«
|}
|}
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*'''Aug-03 We''' [[Image:O2k-Workshops.png|60px|link=MiPNet27.03 IOC154 Innsbruck AT]]  <big>'''[[MiPNet27.03 IOC154 Innsbruck AT|154th Oroboros O2k-Coaching Days - Advanced and NextGen-O2k]]'''</big> Aug 1-5
* '''May-03 Fr'''
:{|
 
| width="110" align="center" | [[File:PlayVideo.jpg|left|80px|O2k-Videosupport|link=O2k-Videosupport]]
 
|''' O<sub>2</sub> flux analysis with DatLab 7.4'''<br>
Use the O<sub>2</sub> analysis template:  <br>


-  »'''[https://www.youtube.com/watch?v=HRAGvmaK01I%7C O2k-Videosupport: O<sub>2</sub> flux analysis with DatLab 7.4]'''«<br>
'''<big>Next Week</big>'''
|}
2024 Week 19
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*'''Aug-04 Th''' [[Image:O2k-Workshops.png|60px|link=MiPNet27.03 IOC154 Innsbruck AT]]  <big>'''[[MiPNet27.03 IOC154 Innsbruck AT|154th Oroboros O2k-Coaching Days - Advanced and NextGen-O2k]]'''</big> Aug 1-5
* '''May-06 Mo'''
:{|
:{|
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
 
|''' What is the effective concentration of digitonin for complete plasma membrane permeabilization?'''<br>
|''' Why do we perform multiple H<sub>2</sub>O<sub>2</sub> calibrations in the Amplex® UltraRed assay?'''<br>
It needs to be determined for each cell type and digitonin batch. The SUIT protocol SUIT-010 was designed to evaluate the optimum digitonin concentration to permeabilize the plasma membrane. <br>
The sensitivity to H<sub>2</sub>O<sub>2</sub> of the fluorometric signal changes during the experiment as a function of: (''1'') experimental time and accumulating UltroxRed (similar to resorufin), (''2'') changes of the optical properties due to titrations, and (''3'') the radical scavenging capacity of the sample. Therefore, H<sub>2</sub>O<sub>2</sub> calibrations are performed before and after sample addition, and after selected titration steps. <br>
- »'''[[Digitonin]]'''«, »'''[[SUIT-010]]'''« <br>
 
- communicated by »[[Schmitt Sabine]]« and »[[Timon-Gomez Alba]]«
- »'''[[Amplex UltraRed]]'''« and  »'''[[Komlodi_2021_BEC_AmR-O2 |Komlodi et al BEC 2021.4]]'''«<br>
|}
|}
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* '''May-07 Tu'''  
*'''Aug-05 Fr''' [[Image:O2k-Workshops.png|60px|link=MiPNet27.03 IOC154 Innsbruck AT]]  <big>'''[[MiPNet27.03 IOC154 Innsbruck AT|154th Oroboros O2k-Coaching Days - Advanced and NextGen-O2k]]'''</big> Aug 1-5
----
:{|
* '''May-08 We'''  
 
----
| width="110" align="center" | [[File:PlayVideo.jpg|left|80px|O2k-Videosupport|link=O2k-Videosupport]]
* '''May-09 Th'''  
 
----
|'''Watch these videos to get started with the Q-Module'''<br>
* '''May-10 Fr'''
 
- »'''[https://wiki.oroboros.at/index.php/O2k-Videosupport#Q-Module_.28NextGen-O2k_-_DatLab_7.29 O2k-Videosupport:Q-Module]'''«<br>
|}

Revision as of 12:04, 29 April 2024

Gentle Science
Oroboros declares solidarity against the Russian aggression-war in Ukraine and against all aggression-wars worldwide. -  »More details
A future for Ukraine is the future of its science
2024 Week 18
  • Apr-29 Mo
O2k-Open SupportInfo: improve How can I update DatLab 8 to the latest version?

If your computer with DatLab 8 is connected to the internet, go to “Check for updates” in the Help menu of DatLab 8, and follow the simple installation instructions.
- »Updating - DatLab«, »DatLab 8«
- communicated by »Leo Elettra« and »Baglivo Eleonora«


  • Apr-30 Tu

  • May-01 We

  • May-02 Th
O2k-Open SupportInfo: improve "In respirometric studies on blood cells, the purity of cell preparation should always be emphasized and documented."

- »MiPNet21.17 BloodCellsIsolation«
- communicated by »Timon-Gomez Alba« and »Baglivo Eleonora«


  • May-03 Fr

Next Week

2024 Week 19
  • May-06 Mo
MitoPedia
What is the effective concentration of digitonin for complete plasma membrane permeabilization?

It needs to be determined for each cell type and digitonin batch. The SUIT protocol SUIT-010 was designed to evaluate the optimum digitonin concentration to permeabilize the plasma membrane.
- »Digitonin«, »SUIT-010« 
- communicated by »Schmitt Sabine« and »Timon-Gomez Alba« 


  • May-07 Tu

  • May-08 We

  • May-09 Th

  • May-10 Fr