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Difference between revisions of "Template:Current week ecosystem agenda"

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{{Template:Science for Ukraine}}
{{Template:Science for Ukraine}}
2023 Week 41


*'''Oct- 09 Mo'''
2024 Week 18
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*'''Oct- 10 Tu'''
* '''Apr-29 Mo'''
:{|
:{|
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
|'''What is the best fluorophore and concentration thereof for measuring mt-membrane potential?'''<br>
|'''How can I update DatLab 8 to the latest version?'''<br>
It depends on sample characteristics -  test the concentration of different fluorophores (safranin, TMRM and rhodamine 123) with your specific model to evaluate for inhibitory or uncoupling effects on respiration. <br>
If your computer with DatLab 8 is connected to the internet, go to “Check for updates” in the Help menu of DatLab 8, and follow the simple installation instructions. <br>
- »'''[https://wiki.oroboros.at/index.php/Mitochondrial_membrane_potential#Different_methods_for_measurements_of_mt-membrane_potential mt-membrane potential measurement]'''« <br>
- »'''[[Updating - DatLab]]'''«, »'''[https://www.oroboros.at/index.php/product/datlab/ DatLab 8]'''«<br>
- communicated by »[[Grings Mateus]]« and »[[Cardoso Luiza]]«  
- communicated by »[[Leo Elettra]]« and »[[Baglivo Eleonora]]«
|}
|}
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*'''Oct- 11 We'''
* '''Apr-30 Tu'''  
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*'''Oct- 12 Th''' - World Arthritis Day
* '''May-01 We'''   
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* '''May-02 Th'''  
:{|
:{|
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
|'''“Mitochondrial dysfunction did not seem to be a major contributing factor for insulin resistance in RA [rheumatoid arthritis]''' <br>
|''' "In respirometric studies on blood cells, the purity of cell preparation should always be emphasized and documented." '''<br>
* Moellering DR, Smith-Johnston K, Kelley C, Sammy MJ, Benedict J, Brock G, Johnson J, Baskin KK, Jarjour WN, Belury MA, Reiser PJ, Nagareddy PR, Hanaoka BY (2023) Association between skeletal muscle mitochondrial dysfunction and insulin resistance in patients with rheumatoid arthritis: a case-control study. [[Moellering 2023 Arthritis Res Ther|»Bioblast link«]]
- »'''[[MiPNet21.17 BloodCellsIsolation]]'''«<br>
:from the O2k-Network »[[US AL Birmingham Moellering_DR]]«
- communicated by »[[Timon-Gomez Alba]]« and »[[Baglivo Eleonora]]«
|}
|}
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* '''May-03 Fr'''


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'''<big>Next Week</big>'''
*'''Oct- 13 Fr'''
  2024 Week 19
----
  2023 Week 42


*'''Oct- 16 Mo'''
* '''May-06 Mo'''
:::: '''»[[2nd Workshop on Mitochondrial Functional Diagnostics Innsbruck AT]]«'''
----
*'''Oct- 17 Tu'''
:{|
:{|
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
|'''Noticing higher values of J°1 when using the O2k sV-chamber (0.5 mL) compared with the 2mL-chamber? '''<br>
|''' What is the effective concentration of digitonin for complete plasma membrane permeabilization?'''<br>
The instrumental oxygen background is volume-dependent in the closed chamber; therefore, the values of J°1 are about four times higher in the sV chamber.  
It needs to be determined for each cell type and digitonin batch. The SUIT protocol SUIT-010 was designed to evaluate the optimum digitonin concentration to permeabilize the plasma membrane. <br>
<br>
- »'''[[Digitonin]]'''«, »'''[[SUIT-010]]'''« <br>
- »'''[[O2k-sV-Module|O2k-sV-Module]]'''«, »'''[[MiPNet06.03 POS-calibration-SOP|POS calibration-SOP]]'''«<br>
- communicated by »[[Schmitt Sabine]]« and »[[Timon-Gomez Alba]]«  
- communicated by »[[Timon-Gomez Alba]]« and »[[Leo Elettra]]«
|}
|}
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*'''Oct- 18 We'''
* '''May-07 Tu'''  
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*'''Oct- 19 Th'''
* '''May-08 We'''  
:{|
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
|'''"MDH2 is a metabolic switch rewiring [electron transfer system] and TCA fuelling"''' <br>
* Molinié T, Cougouilles E, David C, Cahoreau E, Portais JC, Mourier A (2022) MDH2 produced OAA is a metabolic switch rewiring the fuelling of respiratory chain and TCA cycle. [[Molinie 2022 Biochim Biophys Acta Bioenerg|»Bioblast link«]]
:from the O2k-Network »[[FR Bordeaux Mourier A]]«
|}
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*'''Oct- 20 Fr'''
* '''May-09 Th'''  
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* '''May-10 Fr'''

Revision as of 12:04, 29 April 2024

Gentle Science
Oroboros declares solidarity against the Russian aggression-war in Ukraine and against all aggression-wars worldwide. -  »More details
A future for Ukraine is the future of its science
2024 Week 18
  • Apr-29 Mo
O2k-Open SupportInfo: improve How can I update DatLab 8 to the latest version?

If your computer with DatLab 8 is connected to the internet, go to “Check for updates” in the Help menu of DatLab 8, and follow the simple installation instructions.
- »Updating - DatLab«, »DatLab 8«
- communicated by »Leo Elettra« and »Baglivo Eleonora«


  • Apr-30 Tu

  • May-01 We

  • May-02 Th
O2k-Open SupportInfo: improve "In respirometric studies on blood cells, the purity of cell preparation should always be emphasized and documented."

- »MiPNet21.17 BloodCellsIsolation«
- communicated by »Timon-Gomez Alba« and »Baglivo Eleonora«


  • May-03 Fr

Next Week

2024 Week 19
  • May-06 Mo
MitoPedia
What is the effective concentration of digitonin for complete plasma membrane permeabilization?

It needs to be determined for each cell type and digitonin batch. The SUIT protocol SUIT-010 was designed to evaluate the optimum digitonin concentration to permeabilize the plasma membrane.
- »Digitonin«, »SUIT-010« 
- communicated by »Schmitt Sabine« and »Timon-Gomez Alba« 


  • May-07 Tu

  • May-08 We

  • May-09 Th

  • May-10 Fr