Template:Current week ecosystem agenda: Difference between revisions

Revision as of 12:04, 29 April 2024

Oroboros declares solidarity against the Russian aggression-war in Ukraine and against all aggression-wars worldwide. - »More details

-» A future for Ukraine is the future of its science

2024 Week 18

Apr-29 Mo

How can I update DatLab 8 to the latest version?

If your computer with DatLab 8 is connected to the internet, go to “Check for updates” in the Help menu of DatLab 8, and follow the simple installation instructions.
- »Updating - DatLab«, »DatLab 8«
- communicated by »Leo Elettra« and »Baglivo Eleonora«

Apr-30 Tu

May-01 We

May-02 Th

"In respirometric studies on blood cells, the purity of cell preparation should always be emphasized and documented."

- »MiPNet21.17 BloodCellsIsolation«
- communicated by »Timon-Gomez Alba« and »Baglivo Eleonora«

May-03 Fr

Next Week

2024 Week 19

May-06 Mo

What is the effective concentration of digitonin for complete plasma membrane permeabilization?

It needs to be determined for each cell type and digitonin batch. The SUIT protocol SUIT-010 was designed to evaluate the optimum digitonin concentration to permeabilize the plasma membrane.
- »Digitonin«, »SUIT-010«
- communicated by »Schmitt Sabine« and »Timon-Gomez Alba«

May-07 Tu

May-08 We

May-09 Th

May-10 Fr

@@ Line 1: / Line 1: @@
 {{Template:Science for Ukraine}}
-Week 45
-*'''Nov- 06 Mo'''
+Week 18
-----
-*'''Nov- 07 Tu'''
+* '''Apr-29 Mo'''
 :{|
-| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Info: take care]]
+| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
-|'''How often should I re-assemble the O2k-chamber?'''<br>
+|'''How can I update DatLab 8 to the latest version?'''<br>
-We recommend disassembling, cleaning and re-assembling the O2k-chamber at least every 1-2 years. It is also necessary to disassemble it when there is persistent biological or protein contamination, medium leakage from the chamber, an oxygen leak of the chamber, or an unstable or unexpectedly high or low oxygen signal.
+If your computer with DatLab 8 is connected to the internet, go to “Check for updates” in the Help menu of DatLab 8, and follow the simple installation instructions. <br>
-<br>
+- »'''[[Updating - DatLab]]'''«, »'''[https://www.oroboros.at/index.php/product/datlab/ DatLab 8]'''«<br>
-- »'''[https://wiki.oroboros.at/index.php/O2k-chamber#O2k-chamber_assembly_-_possible_problems O2k-chamber assembly - possible problems]'''« and »'''[https://www.youtube.com/watch?v=1MSsI1ePMu8&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=3 O2k-Videosupport: Insert O2k-chamber]'''«<br>
+- communicated by »[[Leo Elettra]]« and »[[Baglivo Eleonora]]«
-- communicated by »[[Timon-Gomez Alba]]« and »[[Schmitt Sabine]]«
 |}
 ----
-*'''Nov- 08 We'''
+* '''Apr-30 Tu'''
 ----
-*'''Nov- 09 Th'''
+* '''May-01 We'''
 ----
-*'''Nov- 10 Fr'''
+* '''May-02 Th'''
 :{|
-| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
+| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
-|''' When should I analyze my data as flux control ratios (''FCR''s)?'''<br>
+|''' "In respirometric studies on blood cells, the purity of cell preparation should always be emphasized and documented." '''<br>
-''FCR''s enable the comparison of samples normalized with various mitochondrial content parameters or when sample concentration is unknown. This is achieved by internally normalizing O<sub><small>2</small></sub> fluxes in different respiratory states to the maximum flux in a common reference state. <br>
+- »'''[[MiPNet21.17 BloodCellsIsolation]]'''«<br>
-- »'''[[Flux control ratio]]'''« <br>
+- communicated by »[[Timon-Gomez Alba]]« and »[[Baglivo Eleonora]]«
-- communicated by »[[Baglivo Eleonora]]« and »[[Timon-Gomez Alba]]«
 |}
 ----
+* '''May-03 Fr'''
 '''<big>Next Week</big>'''
+Week 19
-Week 46
+* '''May-06 Mo'''
-*'''Nov- 13 Mo'''
-----
-*'''Nov- 14 Tu''' - World Diabetes Day
 :{|
-| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
+| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
-|'''"Liver [mitochondria] (...) adapt to metabolic challenges by increasing FAO-derived mitochondrial respiration in OBES [obesity model] and NASH [non-alcoholic steatohepatitis model], but by increasing both FAO- and TCA-derived respiration in DIAB [diabetes model]."''' <br>
+|''' What is the effective concentration of digitonin for complete plasma membrane permeabilization?'''<br>
-* Dewidar B, Mastrototaro L, Englisch C, Ress C, Granata C, Rohbeck E, Pesta D, Heilmann G, Wolkersdorfer M, Esposito I, Reina Do Fundo M, Zivehe F, Yavas A, Roden M (2023) Alterations of hepatic energy metabolism in murine models of obesity, diabetes and fatty liver diseases. [[Dewidar 2023 EBioMedicine|»Bioblast link«]]
+It needs to be determined for each cell type and digitonin batch. The SUIT protocol SUIT-010 was designed to evaluate the optimum digitonin concentration to permeabilize the plasma membrane. <br>
-:from the O2k-Network »[[DE Duesseldorf Roden M]]« and »[[DE Cologne Pesta D]]«
+- »'''[[Digitonin]]'''«, »'''[[SUIT-010]]'''« <br>
+- communicated by »[[Schmitt Sabine]]« and »[[Timon-Gomez Alba]]«
 |}
 ----
-*'''Nov- 15 We'''
+* '''May-07 Tu'''
+----
+* '''May-08 We'''
 ----
-*'''Nov- 16 Th''' - World Pancreatic Cancer Day
+* '''May-09 Th'''
-:{|
-| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
-|'''"To the advantage of PDAC [pancreatic ductal adenocarcinoma] patients, innovative and more effective therapeutics will be possible with a greater understanding of how pancreatic cancer cells control mitochondrial metabolism."''' <br>
-* Padinharayil H, Rai V, George A (2023) Mitochondrial metabolism in pancreatic ductal adenocarcinoma: from mechanism-based perspectives to therapy. [[Padinharayil 2023 Cancers (Basel)|»Bioblast link«]]
-|}
 ----
-*'''Nov- 17 Fr'''
+* '''May-10 Fr'''
----