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Difference between revisions of "Template:Current week ecosystem agenda"

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{{Template:Science for Ukraine}}
{{Template:Science for Ukraine}}
2023 Week 51


*'''Dec- 18 Mo'''
2024 Week 18
:::: '''»[[SBC 2023 Goa IN]]«''' Dec 18-20


* '''Apr-29 Mo'''
:{|
:{|
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
|''' Why add azide after ascorbate and TMPD when measuring Complex IV activity? '''<br>
|'''How can I update DatLab 8 to the latest version?'''<br>
The O<sub><small>2</small></sub> flux measured after the titration of ascorbate and TMPD is due to both CIV activity and autooxidation of these compounds. By subsequently inhibiting CIV activity with azide, the O<sub><small>2</small></sub> flux due to autooxidation (chemical background) can be determined and subtracted from the initial measurement. <br>  
If your computer with DatLab 8 is connected to the internet, go to “Check for updates” in the Help menu of DatLab 8, and follow the simple installation instructions. <br>
- »'''[[Complex IV]]'''«, »'''[[Chemical background]] '''« <br>
- »'''[[Updating - DatLab]]'''«, »'''[https://www.oroboros.at/index.php/product/datlab/ DatLab 8]'''«<br>
- communicated by »[[Grings Mateus]]« and »[[Timon-Gomez Alba]]«  
- communicated by »[[Leo Elettra]]« and »[[Baglivo Eleonora]]«
|}
|}
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*'''Dec- 19 Tu'''
* '''Apr-30 Tu'''  
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* '''May-01 We'''   
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*'''Dec- 20 We'''
* '''May-02 Th'''  
:{|
:{|
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
|'''"SIRT4 [Sirtuin 4] knockdown increases mitochondrial capacity to oxidize fatty acids, decreasing LEAK respiration and further increasing mitochondrial elongation via its regulation of mitochondrial fusion."''' <br>
|''' "In respirometric studies on blood cells, the purity of cell preparation should always be emphasized and documented." '''<br>
* Noone J, Rochfort KD, O'Sullivan F, O'Gorman DJ (2023) SIRT4 is a regulator of human skeletal muscle fatty acid metabolism influencing inner and outer mitochondrial membrane-mediated fusion. [[Noone 2023 Cell Signal|»Bioblast link«]]
- »'''[[MiPNet21.17 BloodCellsIsolation]]'''«<br>
:from the O2k-Network »[[US FL Orlando Goodpaster BH]]« and »[[IE Dublin O Gorman D]]«
- communicated by »[[Timon-Gomez Alba]]« and »[[Baglivo Eleonora]]«
|}
|}
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*'''Dec- 21 Th'''
* '''May-03 Fr'''
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*'''Dec- 22 Fr'''
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'''<big>Next Week</big>'''
'''<big>Next Week</big>'''
  2023 Week 52
  2024 Week 19


*'''Dec- 25 Mo'''
* '''May-06 Mo'''
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*'''Dec- 26 Tu'''
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*'''Dec- 27 We'''
:{|
:{|
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
|'''Do you observe a high signal at zero oxygen?'''<br>
|''' What is the effective concentration of digitonin for complete plasma membrane permeabilization?'''<br>
If the quality of your dithionite solution has already been checked, perform a leak current test to evaluate which electrical component is generating the out-of-scale signal.  
It needs to be determined for each cell type and digitonin batch. The SUIT protocol SUIT-010 was designed to evaluate the optimum digitonin concentration to permeabilize the plasma membrane. <br>
<br>
- »'''[[Digitonin]]'''«, »'''[[SUIT-010]]'''« <br>
- »'''[https://wiki.oroboros.at/index.php/High_signal_at_zero_oxygen Leak current test]'''«<br>
- communicated by »[[Schmitt Sabine]]« and »[[Timon-Gomez Alba]]«  
- communicated by »[[Baglivo Eleonora]]« and »[[Grings Mateus]]«
|}
|}
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*'''Dec- 28 Th'''
* '''May-07 Tu'''
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* '''May-08 We'''  
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*'''Dec- 29 Fr'''
* '''May-09 Th'''  
:{|
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
|'''"Leptin receptor signaling in Schwann cells adjusts catabolic processes, including mitochondrial respiration and autophagy, facilitating nerve repair."''' <br>
* Sundaram VK, Schütza V, Schröter NH, Backhaus A, Bilsing A, Joneck L, Seelbach A, Mutschler C, Gomez-Sanchez JA, Schäffner E, Sánchez EE, Akkermann D, Paul C, Schwagarus N, Müller S, Odle A, Childs G, Ewers D, Kungl T, Sitte M, Salinas G, Sereda MW, Nave KA, Schwab MH, Ost M, Arthur-Farraj P, Stassart RM, Fledrich R (2023) Adipo-glial signaling mediates metabolic adaptation in peripheral nerve regeneration. [[Sundaram_2023_Cell_Metab|»Bioblast link«]]
:from the O2k-Network »[[DE_Leipzip_Ost_M]]«
|}
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* '''May-10 Fr'''

Revision as of 12:04, 29 April 2024

Gentle Science
Oroboros declares solidarity against the Russian aggression-war in Ukraine and against all aggression-wars worldwide. -  »More details
A future for Ukraine is the future of its science
2024 Week 18
  • Apr-29 Mo
O2k-Open SupportInfo: improve How can I update DatLab 8 to the latest version?

If your computer with DatLab 8 is connected to the internet, go to “Check for updates” in the Help menu of DatLab 8, and follow the simple installation instructions.
- »Updating - DatLab«, »DatLab 8«
- communicated by »Leo Elettra« and »Baglivo Eleonora«


  • Apr-30 Tu

  • May-01 We

  • May-02 Th
O2k-Open SupportInfo: improve "In respirometric studies on blood cells, the purity of cell preparation should always be emphasized and documented."

- »MiPNet21.17 BloodCellsIsolation«
- communicated by »Timon-Gomez Alba« and »Baglivo Eleonora«


  • May-03 Fr

Next Week

2024 Week 19
  • May-06 Mo
MitoPedia
What is the effective concentration of digitonin for complete plasma membrane permeabilization?

It needs to be determined for each cell type and digitonin batch. The SUIT protocol SUIT-010 was designed to evaluate the optimum digitonin concentration to permeabilize the plasma membrane.
- »Digitonin«, »SUIT-010« 
- communicated by »Schmitt Sabine« and »Timon-Gomez Alba« 


  • May-07 Tu

  • May-08 We

  • May-09 Th

  • May-10 Fr