Template:Keywords: Coupling control: Difference between revisions

Revision as of 23:49, 10 November 2020

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The Blue Book 2020
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OXPHOS-, ROUTINE-, ET-, and LEAK states; respiratory capacities (P, R, E, L) corrected for residual oxygen consumption Rox.
4-compartmental OXPHOS model. (1) ET capacity E of the noncoupled electron transfer system ETS. OXPHOS capacity P is partitioned into (2) the dissipative LEAK component L, and (3) ADP-stimulated P-L net OXPHOS capacity. (4) If P-L is kinetically limited by a low capacity of the phosphorylation system to utilize the protonmotive force pmF, then the apparent E-P excess capacity is available to drive coupled processes other than phosphorylation P» (ADP to ATP) without competing with P».
(P-L)/P is the OXPHOS P-L control efficiency. The biochemical coupling efficiency is independent of kinetic control by the phosphorylation system when expressed as the E-L coupling efficiency, (E-L)/E. (E-P)/E is the kinetic E-P control efficiency.

Bioblast links: Coupling control - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>

Mitochondrial and cellular respiratory rates in coupling-control states

Respiratory rate	Definition
OXPHOS capacity	P = P´-Rox
ROUTINE respiration	R = R´-Rox
ET capacity	E = E´-Rox	» Level flow
		» Noncoupled respiration - Uncoupler^[1]
LEAK respiration	L = L´-Rox	» Static head
		» LEAK state with ATP
		» LEAK state with oligomycin
		» LEAK state without adenylates
Residual oxygen consumption Rox	L = L´-Rox

Chance and Williams nomenclature: respiratory states

» State 1 —» State 2 —» State 3 —» State 4 —» State 5

Flux-control ratios related to coupling in mt-preparations and living cells

» Flux-control ratio

» Coupling-control ratio

» Coupling-control protocol

FCR	Definition
L/P coupling-control ratio	L/P	» Respiratory acceptor control ratio, RCR = P/L
L/R coupling-control ratio	L/R
L/E coupling-control ratio	L/E	» Uncoupling-control ratio, UCR = E/L
P/E control ratio	P/E
R/E control ratio	R/E
net P/E control ratio	(P-L)/E
net R/E control ratio	(R-L)/E

Net, excess, and reserve capacities of respiration

Respiratory net rate	Definition	Icon
Net OXPHOS capacity P-L	P-L
Net ROUTINE activity R-L
Net ET capacity E-L	E-L
ET-excess capacity E-P	E-P
ET-reserve capacity E-R	E-R

Flux-control efficiencies related to coupling-control ratios

» Flux-control efficiency j_Z-Y

» Background state

» Reference state

» Metabolic control variable

Coupling-control efficiency		Definition
OXPHOS-coupling efficiency P-L	j_P-L	= (P-L)/P	= 1-L/P	» Biochemical coupling efficiency
ROUTINE-coupling efficiency R-L	j_R-L	= (R-L)/R	= 1-L/R
ET-coupling efficiency E-L	j_E-L	= (E-L)/E	= 1-L/E	» Biochemical coupling efficiency
ET-excess control efficiency E-P	j_E-P	= (E-P)/E	= 1-P/E
ET-reserve control efficiency E-R	j_E-R	= (E-R)/E	= 1-R/E

General

» Basal respiration

» Dyscoupled respiration

» Dyscoupling

» Electron leak

» Electron-transfer-pathway state

» Oxidative phosphorylation

» Oxygen flow

» Oxygen flux

» Permeabilized cells

» Phosphorylation system

» Proton leak

» Proton slip

» Respiratory state

» Uncoupling

↑ Gnaiger E. Is respiration uncoupled - noncoupled - dyscoupled? Mitochondr Physiol Network. »Uncoupler«

[1] Gnaiger E. Is respiration uncoupled - noncoupled - dyscoupled? Mitochondr Physiol Network. »Uncoupler«

[1]

@@ Line 15: / Line 15: @@
 ! Respiratory rate !! Definition !! Icon
 |-
-| [[OXPHOS-capacity]] || ''P'' = ''P´''-''Rox'' || [[File:P.jpg |link=OXPHOS-capacity]]
+| [[OXPHOS capacity]] || ''P'' = ''P´''-''Rox'' || [[File:P.jpg |link=OXPHOS capacity]]
 |-
-| [[ROUTINE-respiration]] || ''R'' = ''R´''-''Rox'' || [[File:R.jpg |link=ROUTINE-respiration]]
+| [[ROUTINE respiration]] || ''R'' = ''R´''-''Rox'' || [[File:R.jpg |link=ROUTINE respiration]]
 |-
-| [[ET-capacity]] || ''E'' = ''E´''-''Rox'' || [[File:E.jpg |link=ET-capacity]] || » [[Level flow]]
+| [[ET capacity]] || ''E'' = ''E´''-''Rox'' || [[File:E.jpg |link=ET capacity]] || » [[Level flow]]
 |-
 |  ||  ||  || » [[Noncoupled respiration]] - [[Uncoupler]]<ref>Gnaiger E. Is respiration uncoupled - noncoupled - dyscoupled? Mitochondr Physiol Network. »[[Uncoupler]]«</ref>
 |-
-| [[LEAK-respiration]] || ''L'' = ''L´''-''Rox'' || [[File:L.jpg |link=LEAK-respiration]] || » [[Static head]]
+| [[LEAK respiration]] || ''L'' = ''L´''-''Rox'' || [[File:L.jpg |link=LEAK respiration]] || » [[Static head]]
 |-
-|  ||  ||  || » [[LEAK-state with ATP]]
+|  ||  ||  || » [[LEAK state with ATP]]
 |-
-|  ||  ||  || » [[LEAK-state with oligomycin]]
+|  ||  ||  || » [[LEAK state with oligomycin]]
 |-
-|  ||  ||  || » [[LEAK-state without adenylates]]
+|  ||  ||  || » [[LEAK state without adenylates]]
 |-
 | [[Residual oxygen consumption]] ''Rox'' || ''L'' = ''L´''-''Rox'' || [[File:ROX.jpg |link=Residual oxygen consumption]]
@@ Line 66: / Line 66: @@
 ! Respiratory net rate !! Definition !! Icon
 |-
-| [[Net OXPHOS-capacity P-L |Net OXPHOS-capacity ''P-L'']] || ''P-L'' || [[Image:P-L.jpg|50 px|link=Net OXPHOS-capacity P-L |Net OXPHOS-capacity ''P-L'']]
+| [[Net OXPHOS capacity P-L |Net OXPHOS capacity ''P-L'']] || ''P-L'' || [[Image:P-L.jpg|50 px|link=Net OXPHOS capacity P-L |Net OXPHOS  capacity ''P-L'']]
 |-
-| [[Net ROUTINE-activity R-L |Net ROUTINE-activity ''R-L'']] ||  || [[Image:R-L.jpg|50 px|link=Net ROUTINE-activity R-L |Net ROUTINE-activity ''R-L'']]
+| [[Net ROUTINE activity R-L |Net ROUTINE activity ''R-L'']] ||  || [[Image:R-L.jpg|50 px|link=Net ROUTINE activity R-L |Net ROUTINE activity ''R-L'']]
 |-
-| [[Net ET-capacity E-L |Net ET-capacity ''E-L'']] || ''E-L'' || [[Image:E-L.jpg|50 px|link=Net ET-capacity E-L |Net ET-capacity ''E-L'']]
+| [[Net ET capacity E-L |Net ET capacity ''E-L'']] || ''E-L'' || [[Image:E-L.jpg|50 px|link=Net ET capacity E-L |Net ET capacity ''E-L'']]
 |-
 | [[ET-excess capacity E-P |ET-excess capacity ''E-P'']] || ''E-P'' || [[Image:E-P.jpg|60 px|link=ET-excess capacity E-P |ET-excess capacity ''E-P'']]