Template:MitoPediaAlert2021: Difference between revisions

Revision as of 09:22, 15 March 2021

Currently Answered Questions from the »Oroboros Ecosystem Agenda«

Mar 2021

Mar-16 Tu

CI inhibitors like rotenone inhibit fatty acid oxidation (FAO)-linked respiration

Inhibition of CI leads to accumulation of NADH in the mt-matrix. The resultant lack of NAD⁺ blocks FAO.
- »Fatty acid oxidation pathway control state«, »Mitochondrial Pathways: Chapter 5«
- communicated by »Cardoso Luiza« and »Cecatto Cristiane«

Mar-11 Th

Internal normalization of rate – what is the difference between flux control ratio and flux control efficiency?

A flux control ratio is the ratio of oxygen flux in a specific respiratory control state, normalized for maximum flux in a common reference state. Flux control efficiency expresses the control of respiration as a fractional change of oxygen flux or flow between protocol steps. Both values vary between theoretical lower and upper limits of 0 and 1.
- »Gnaiger 2020 BEC MitoPathways - Chapter 3«
- » Posted by: »Komlodi Timea« and »Cecatto Cristiane«

Mar-2 Tu

Measuring fatty acid oxidation (FAO)-linked respiration requires a low concentration of malate

In the absence of malate, accumulation of Acetyl-CoA inhibits FAO.
- »Fatty acid oxidation pathway control state«, »Mitochondrial Pathways: Chapter 5«
- communicated by »Cecatto Cristiane« and »Cardoso Luiza«

Jan 2021

Jan-21 Th

Measuring ATP production with Magnesium Green – which sample preparations work?

Magnesium Green (MgG) can be used with mitochondrial preparations: isolated mitochondria, tissue homogenates, and permeabilized cells and tissues.
- »MgG« and »Publications MgG«
- communicated by »Cardoso Luiza« and »Komlodi Timea«

@@ Line 2: / Line 2: @@
 </big>__TOC__
 == Mar 2021 ==
+*'''Mar-16 Tu'''
+:{|
+| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
+|''' CI inhibitors like rotenone inhibit fatty acid oxidation (FAO)-linked respiration'''<br>
+Inhibition of CI leads to accumulation of NADH in the mt-matrix. The resultant lack of NAD<sup>+</sup> blocks FAO. <br>
+- »'''[[Fatty acid oxidation pathway control state]]'''«, »'''[https://wiki.oroboros.at/images/e/ec/BEC_2020.2_doi10.26124bec2020-0002.pdf Mitochondrial Pathways: Chapter 5]'''« <br>
+- communicated by »[[Cardoso Luiza]]« and »[[Cecatto Cristiane]]«
+|}
+----
+*'''Mar-11 Th'''
+:{|
+| width="110" align="center" | [[File:Gnaiger 2020 BEC MitoPathways.jpg|70px|link=https://www.bioenergetics-communications.org/index.php/bec/article/view/gnaiger_2020_mitopathways|Gnaiger 2020 BEC MitoPathways]]
+| '''Internal normalization of rate  – what is the difference between ''flux control ratio'' and ''flux control efficiency''?'''<br>
+A ''flux control ratio'' is the ratio of oxygen flux in a specific respiratory control state, normalized for maximum flux in a common reference state. ''Flux control efficiency'' expresses the control of respiration as a fractional change of oxygen flux or flow between protocol steps. Both values vary between theoretical lower and upper limits of 0 and 1. <br>
+- »[https://www.bioenergetics-communications.org/index.php/bec/article/view/gnaiger_2020_mitopathways Gnaiger 2020 BEC MitoPathways - Chapter 3]« <br>
+- » Posted by: »[[Komlodi Timea]]« and »[[Cecatto Cristiane]]«
+|}
+----
 *'''Mar-2 Tu'''