Template:MitoPediaAlert2021: Difference between revisions
Tindle Lisa (talk | contribs) No edit summary |
Tindle Lisa (talk | contribs) No edit summary |
||
Line 1: | Line 1: | ||
:::::: <big>''Currently Answered Questions from the Β»[[OROBOROS_INSTRUMENTS|Oroboros Ecosystem Agenda]]Β« | :::::: <big>''Currently Answered Questions from the Β»[[OROBOROS_INSTRUMENTS|Oroboros Ecosystem Agenda]]Β« | ||
</big>__TOC__ | </big>__TOC__ | ||
== May 2021 == | |||
*'''May-11 Tu''' | |||
:{| | |||
| width="100" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]] | |||
|''' Measuring succinate pathway (S-pathway) in living cells '''<br> | |||
Using [[MitoKit-CII]] β cell permeable succinate and malonate, the [[Succinate pathway|S-pathway]] can be measuredΒ <br> | |||
- Β»'''[[SUIT-003 O2 ce D060]]'''Β«<br> | |||
- communicated by Β»[[Cecatto Cristiane]]Β« and Β»[[Komlodi Timea]]Β« | |||
|} | |||
== Apr 2021 == | |||
== Mar 2021 == | == Mar 2021 == | ||
*'''Mar-16 Tu''' Β | *'''Mar-16 Tu''' Β | ||
:{| | :{| |
Revision as of 08:38, 10 May 2021
- Currently Answered Questions from the Β»Oroboros Ecosystem AgendaΒ«
May 2021
- May-11 Tu
Measuring succinate pathway (S-pathway) in living cells
Using MitoKit-CII β cell permeable succinate and malonate, the S-pathway can be measured
- Β»SUIT-003 O2 ce D060Β«
- communicated by Β»Cecatto CristianeΒ« and Β»Komlodi TimeaΒ«
Apr 2021
Mar 2021
- Mar-16 Tu
CI inhibitors like rotenone inhibit fatty acid oxidation (FAO)-linked respiration
Inhibition of CI leads to accumulation of NADH in the mt-matrix. The resultant lack of NAD+ blocks FAO.
- Β»Fatty acid oxidation pathway control stateΒ«, Β»Mitochondrial Pathways: Chapter 5Β«
- communicated by Β»Cardoso LuizaΒ« and Β»Cecatto CristianeΒ«
- Mar-11 Th
Internal normalization of rate β what is the difference between flux control ratio and flux control efficiency?
A flux control ratio is the ratio of oxygen flux in a specific respiratory control state, normalized for maximum flux in a common reference state. Flux control efficiency expresses the control of respiration as a fractional change of oxygen flux or flow between protocol steps. Both values vary between theoretical lower and upper limits of 0 and 1.
- Β»Gnaiger 2020 BEC MitoPathways - Chapter 3Β«
- Β» Posted by: Β»Komlodi TimeaΒ« and Β»Cecatto CristianeΒ«
- Mar-2 Tu
Measuring fatty acid oxidation (FAO)-linked respiration requires a low concentration of malate
In the absence of malate, accumulation of Acetyl-CoA inhibits FAO.
- Β»Fatty acid oxidation pathway control stateΒ«, Β»Mitochondrial Pathways: Chapter 5Β«
- communicated by Β»Cecatto CristianeΒ« and Β»Cardoso LuizaΒ«
Jan 2021
- Jan-21 Th
Measuring ATP production with Magnesium Green β which sample preparations work?
Magnesium Green (MgG) can be used with mitochondrial preparations: isolated mitochondria, tissue homogenates, and permeabilized cells and tissues.
- Β»MgGΒ« and Β»Publications MgGΒ«
- communicated by Β»Cardoso LuizaΒ« and Β»Komlodi TimeaΒ«