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Difference between revisions of "Template:Next oroboros ecosystem agenda"

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The sensitivity to H<sub>2</sub>O<sub>2</sub> of the fluorometric signal changes during the experiment as a function of: (''1'') experimental time and accumulating UltroxRed (similar to resorufin), (''2'') changes of the optical properties due to titrations, and (''3'') the radical scavenging capacity of the sample. Therefore, H<sub>2</sub>O<sub>2</sub> calibrations are performed before and after sample addition, and after selected titration steps.  <br>
The sensitivity to H<sub>2</sub>O<sub>2</sub> of the fluorometric signal changes during the experiment as a function of: (''1'') experimental time and accumulating UltroxRed (similar to resorufin), (''2'') changes of the optical properties due to titrations, and (''3'') the radical scavenging capacity of the sample. Therefore, H<sub>2</sub>O<sub>2</sub> calibrations are performed before and after sample addition, and after selected titration steps.  <br>


- »'''[[Amplex UltraRed]]'''« and  »'''[[Komlodi_2021_BEC_AmR-O2 |Komlodie et al BEC 2021.4]]'''«<br>
- »'''[[Amplex UltraRed]]'''« and  »'''[[Komlodi_2021_BEC_AmR-O2 |Komlodi et al BEC 2021.4]]'''«<br>
|}
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Revision as of 07:51, 1 August 2022


2022 Week 31
O2k-Open SupportInfo: improve O2k-checklist: Get started with an O2k-experiment

The O2k-checklist provides an overview of all essential steps for starting an O2k-experiment.
- » MiPNet20.04_O2k-checklist« 
- » communicated by »Cecatto Cristiane« and »Schmitt Sabine«


MitoPedia: SUIT What are the substrate-uncoupler-inhibitor titration reference protocols?

These protocols provide a common baseline for comparison of mitochondrial respiratory control in a large variety of species, tissues and cell types, mt-preparations and laboratories
- » SUIT reference protocol«

- communicated by »Cardoso Luiza HD« and »Tindle-Solomon Lisa«


O2k-Videosupport
O2 flux analysis with DatLab 7.4

Use our latest O2 analysis template:

-  »O2k-Videosupport: O2 flux analysis with DatLab 7.4«


MitoPedia
Why do we perform multiple H2O2 calibrations in the Amplex® UltraRed assay?

The sensitivity to H2O2 of the fluorometric signal changes during the experiment as a function of: (1) experimental time and accumulating UltroxRed (similar to resorufin), (2) changes of the optical properties due to titrations, and (3) the radical scavenging capacity of the sample. Therefore, H2O2 calibrations are performed before and after sample addition, and after selected titration steps.

- »Amplex UltraRed« and  »Komlodi et al BEC 2021.4«


O2k-Videosupport
Watch these videos to get started with the Q-Module

- »O2k-Videosupport:Q-Module«

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O2k-Workshops.png 155th Oroboros O2k-Workshop on high-resolution respirometry (HRR) Oct 3-8

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O2k-Workshops.png 155th Oroboros O2k-Workshop on high-resolution respirometry (HRR) Oct 3-8

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O2k-Workshops.png 155th Oroboros O2k-Workshop on high-resolution respirometry (HRR) Oct 3-8

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O2k-Workshops.png 155th Oroboros O2k-Workshop on high-resolution respirometry (HRR) Oct 3-8

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