Difference between revisions of "Template:O2k-OpenSupportAlert2021"
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== May 2021 == | == May 2021 == | ||
*'''May-17 Mo''' | |||
:{| | |||
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]] | |||
|''' Planning to measure proton flux or mitochondrial membrane potential with ion-selective electrodes in the O2k?'''<br> | |||
Learn how to assemble the reference electrodes: | |||
<br> | |||
- »'''[https://www.youtube.com/watch?v=LlIKDFDDeyQ| O2k-Videosupport: Assembly of the reference electrode]'''« | |||
<br> | |||
- communicated by »[[Cardoso Luiza]]« and »[[Schmitt Sabine]]« | |||
|} | |||
---- | |||
*'''May-04 Tu''' | *'''May-04 Tu''' | ||
:{| | :{| | ||
Revision as of 08:18, 17 May 2021
- Currently Answered Questions from the »Oroboros Ecosystem Agenda«
May 2021
- May-17 Mo
Planning to measure proton flux or mitochondrial membrane potential with ion-selective electrodes in the O2k?
Learn how to assemble the reference electrodes:
- »O2k-Videosupport: Assembly of the reference electrode«
- communicated by »Cardoso Luiza« and »Schmitt Sabine«
- May-04 Tu
-
Do you know how to apply the air and zero calibration values in DatLab?
This important quality control step ensures accurate measurement of respiration.
For details on how to apply air and zero calibration in DatLab, see:
»Oxygen calibration - DatLab« and »Troubleshooting«
- communicated by »Komlodi Timea« and »Cecatto Cristiane«
Apr 2021
- Apr-27 Tu
Why is the fluorescence signal constantly at 10 V or at 0 V?
Follow these steps to localize and solve the problem:
- »Fluorescence signal is constantly at 10 V« and »0 V fluorescence signal«
- Apr-20 Tu
Noisy fluorescence signal?
Follow these steps to localize and solve the problem:
- »Noisy fluorescence signal«
- Apr-13 Tu
Do you know what to do when the fluorescence signal starts at a negative value?
A negative signal may be caused by wet Fluo-Sensors. For further information:
- »Negative fluorescence signal«
- Apr-06 Tu
-
A damaged OroboPOS-Holder may cause medium to leak from the O2k-Chamber.
See how you can check that the OroboPOS-Holders are intact.
- »O2k-OroboPOS-Holder«
Mar 2021
- Mar-25 Th
-
O2k-checklist: Get started with an O2k-experiment
The O2k-checklist provides an overview of all essential steps for starting an O2k-experiment.
- » MiPNet20.04_O2k-checklist«
- » communicated by »Cecatto Cristiane« and »Schmitt Sabine«
Feb 2021
- Feb-17 We
-
Do you know how to prepare permeabilized muscle fibers? The application of high-resolution respirometry in permeabilized muscle fibers provides a sensitive diagnostic tool for the analysis of mitochondrial (dys)function in small biopsy samples.
- »O2k-Videosupport: Fiber permeabilization«
- »Doerrier 2018 Methods Mol Biol«
- communicated by »Doerrier Carolina« and »Tindle-Solomon Lisa«
- Feb-08 Mo
-
Do you know how to properly insert the ISS-tip into the O2k-chamber?
With the stirrer bar still rotating, insert the ISS-tip to the bottom of the O2k-Chamber, so that it points away from you.
- »MiPNet19.03 O2k-cleaning and ISS: Section 2«
- communicated by »Doerrier Carolina«, »Cecatto Cristiane« and »Komlodi Timea«
- Feb-02 Tu
-
DatLab error messages: What do they mean and how to solve them?
- » DatLab error messages«
- communicated by »Garcia-Souza Luiz F« and »Tindle-Solomon Lisa«
Jan 2021
- Jan-11 Mo
-
Are you a beginner or need a refresher on using SUIT protocols with DatLab7.4?
- » O2k-Videosupport: How to perform an experiment with a SUIT DL-Protocol« and » Run DL-Protocol/Set O2 limit in DatLab «
- communicated by »Komlodi Timea« and Schmitt Sabine«
