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Difference between revisions of "Template:SUIT-001"

From Bioblast
Line 5: Line 5:
! Respiratory state
! Respiratory state
! Pathway control
! Pathway control
! Pathway to Q
! ET-Complex entry into Q-junction
! Comment


|-
|-
| 1PM
| 1PM
| [[PM]](L)
| [[PM]]<sub>''L''</sub>
| [[N]]
| [[N]]
| CI
| CI
| PM<sub>''L''</sub> or PM_L: Pyruvate & Malate, N-LEAK respiration, N<sub>''L''</sub>
NADH-linked substrates (type N; CI-linked pathway to Q). Non-phosphorylating resting state (LEAK state); ''L''<sub>n</sub> in the absence of ADP, ATP, AMP (no adenylates).


|-
|-
| 2D
| 2D
| [[PM]](P)
| [[PM]]<sub>''P''</sub>
| [[N]]
| [[N]]
| CI
| CI
| PM<sub>''P''</sub> or PM_P: N-OXPHOS capacity, N<sub>''P''</sub>
OXPHOS capacity, ''P'' (with saturating [ADP]), with NADH-linked substrates.


|-
|-
| 2c
| 2c
| [[PM]](P)c
| [[PM]]c<sub>''P''</sub>
| [[N]]
| [[N]]
| CI
| CI
| PMc<sub>''P''</sub> or PMc_P: Cytochrome c test for quality control
Addition of cytochrome ''c'' yields a test for integrity of the [[mtOM]]. Stimulation by added cytochrome ''c'' would indicate an injury of the mtOM and limitation of respiration in state N<sub>''P''</sub> due to loss of cytochrome ''c'' (''P'', OXPHOS capacity with type N substrates). Cytochrome ''c'' is added immediately after the earliest ADP-activation step.


|-
|-
| 3U
| 3U
| [[PM]](E)
| [[PM]]<sub>''E''</sub>
| [[N]]
| [[N]]
| CI
| CI
| PM<sub>''E''</sub> or PM_E: N-ET capacity, N<sub>''E''</sub>
Uncoupler titration (avoiding inhibition by high uncoupler concentrations) to obtain electron transfer (ET) capacity (noncoupled ET-state), as a test for limitation of OXPHOS-capacity by the phosphorylation system (ANT, ATP synthase, phosphate transporter) relative to ET-capacity, E.


|-
|-
Line 39: Line 52:
|-
|-
| 5S
| 5S
| [[PGMS]](E)
| [[PGMS]]<sub>''E''</sub>
| [[NS]]
| [[NS]]
| CI&II
| CI&II
| GMS<sub>''P''</sub> or GMS_P: NS-OXPHOS capacity, NS<sub>''P''</sub>
Respiratory stimulation by further addition of succinate, S, to type N substrates, with convergent electron flow in the NS-pathway (CI<small>&</small>II-linked pathway to the Q-junction) for reconstitution of TCA cycle function, in the coupled state as an estimate of OXPHOS-capacity, ''P''.


|-
|-
Line 51: Line 67:
|-
|-
| 7Rot
| 7Rot
| [[S]](E)
| [[S]]<sub>''E''</sub>
| [[S]]
| [[S]]
| CII
| CII
| S<sub>''E''</sub> or S_E: S-ET capacity
S-pathway ET-capacity after blocking CI with rotenone, inhibiting the N- and F-pathways.


|-
|-
Line 63: Line 82:
|-
|-
| 9Ama
| 9Ama
|
| ROX
| ROX
|-
| 10Tm
| [[Tm]](E)
| [[CIV]]
| CIV
|-
| 11Azd
|
| ROX
| ROX
|}
[[SUIT-011]]
{| class="wikitable" border="1"
|-
! Step
! Respiratory state
! Pathway control
! ET-Complex entry into Q-junction
! Comment
|-
| 1GM
| [[GM]]<sub>''L''</sub>
| [[N]]
| CI
| GM<sub>''L''</sub> or GM_L: N-LEAK respiration, N<sub>''L''</sub>
NADH-linked substrates glutamate & malate (type N; CI-linked pathway to Q). Non-phosphorylating resting state (LEAK state); ''L''<sub>n</sub> in the absence of ADP, ATP, AMP (no adenylates).
|-
| 2D
| [[GM]]<sub>''P''</sub>
| [[N]]
| CI
| GM<sub>''P''</sub> or GM_P: N-OXPHOS capacity, N<sub>''P''</sub>
OXPHOS capacity, ''P'' (with saturating [ADP]), with NADH-linked substrates glutamate&malate.
|-
| D(c)
| [[GM]]c<sub>''P''</sub>
| [[N]]
| CI
| GMc<sub>''P''</sub> or GMc_P: Cytochrome c test for quality control
Addition of cytochrome ''c'' yields a test for integrity of the [[mtOM]]. Stimulation by added cytochrome ''c'' would indicate an injury of the mtOM and limitation of respiration in state GM<sub>''P''</sub> due to loss of cytochrome ''c'' (''P'', OXPHOS capacity with type N substrates). Cytochrome ''c'' is added immediately after the earliest ADP-activation step.
|-
| 3S
| [[GMS]]<sub>''P''</sub>
| [[NS]]
| CI&II
| GMS<sub>''P''</sub> or GMS_P: NS-OXPHOS capacity, NS<sub>''P''</sub>
Respiratory stimulation by further addition of succinate, S, to type N substrates, with convergent electron flow in the NS-pathway (CI<small>&</small>II-linked pathway to the Q-junction) for reconstitution of TCA cycle function, in the coupled state as an estimate of OXPHOS-capacity, ''P''.
|-
| 4U
| [[GMS]]<sub>''E''</sub>
| [[NS]]
| CI&II
| GMS<sub>''E''</sub> or GMS_E: NS-ET capacity, NS<sub>''E''</sub>
Uncoupler titration (avoiding inhibition by high uncoupler concentrations) to obtain electron transfer (ET) capacity (noncoupled ET-state), as a test for limitation of OXPHOS-capacity by the phosphorylation system (ANT, ATP synthase, phosphate transporter) relative to ET-capacity, E.
|-
| 5Rot
| [[S]]<sub>''E''</sub>
| [[S]]
| CII
| S<sub>''E''</sub> or S_E: S-ET capacity
S-pathway ET-capacity after blocking CI with rotenone.
|-
| 6Ama
| [[ROX]]
| [[ROX]]
|  
|  
Line 152: Line 88:


''Rox'' is due to oxidative side reactions, estimated after addition of Antimycin A (inhibitor of CIII). ''Rox'' is subtracted from oxygen flux as a baseline for all respiratory states, to obtain mitochondrial respiration (mt).
''Rox'' is due to oxidative side reactions, estimated after addition of Antimycin A (inhibitor of CIII). ''Rox'' is subtracted from oxygen flux as a baseline for all respiratory states, to obtain mitochondrial respiration (mt).
|}
|}

Revision as of 10:26, 11 January 2019

SUIT-001

Step Respiratory state Pathway control ET-Complex entry into Q-junction Comment
1PM PML N CI PML or PM_L: Pyruvate & Malate, N-LEAK respiration, NL

NADH-linked substrates (type N; CI-linked pathway to Q). Non-phosphorylating resting state (LEAK state); Ln in the absence of ADP, ATP, AMP (no adenylates).

2D PMP N CI PMP or PM_P: N-OXPHOS capacity, NP

OXPHOS capacity, P (with saturating [ADP]), with NADH-linked substrates.

2c PMcP N CI PMcP or PMc_P: Cytochrome c test for quality control

Addition of cytochrome c yields a test for integrity of the mtOM. Stimulation by added cytochrome c would indicate an injury of the mtOM and limitation of respiration in state NP due to loss of cytochrome c (P, OXPHOS capacity with type N substrates). Cytochrome c is added immediately after the earliest ADP-activation step.

3U PME N CI PME or PM_E: N-ET capacity, NE

Uncoupler titration (avoiding inhibition by high uncoupler concentrations) to obtain electron transfer (ET) capacity (noncoupled ET-state), as a test for limitation of OXPHOS-capacity by the phosphorylation system (ANT, ATP synthase, phosphate transporter) relative to ET-capacity, E.

4G PGM(E) N CI
5S PGMSE NS CI&II GMSP or GMS_P: NS-OXPHOS capacity, NSP

Respiratory stimulation by further addition of succinate, S, to type N substrates, with convergent electron flow in the NS-pathway (CI&II-linked pathway to the Q-junction) for reconstitution of TCA cycle function, in the coupled state as an estimate of OXPHOS-capacity, P.

6Oct OctPGMS(E) FNS FAO&CI&II
7Rot SE S CII SE or S_E: S-ET capacity

S-pathway ET-capacity after blocking CI with rotenone, inhibiting the N- and F-pathways.

8Gp SGp(E) SGp CII&GpDH
9Ama ROX ROX: residual oxygen consumption

Rox is due to oxidative side reactions, estimated after addition of Antimycin A (inhibitor of CIII). Rox is subtracted from oxygen flux as a baseline for all respiratory states, to obtain mitochondrial respiration (mt).

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