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Difference between revisions of "Template talk:Next oroboros ecosystem agenda"

From Bioblast
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== O2k-Open Support alert circulars ==
== O2k-Open Support alert circulars ==
=== O2k-Open Support alert circular 2020(07): in prep ===
:{|
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]
| '''Do you know how to select the proper Fluorescence-Sensor and filter set?''' <br>
Using different Fluorescence-Sensors and [[Filters|filters]], the [https://www.oroboros.at/index.php/product/o2k-fluo-smart-module/ O2k-Fluo Smart-Module] allows for measurement of [[Hydrogen peroxide|hydrogen peroxide]], [[ATP]], [[Mitochondrial membrane potential|mitochondrial membrane potential]], [[Calcium|calcium]], and numerous other applications open for O2k-user innovation.<br>
- »'''[[O2k-Fluo_Smart-Module#Select_the_Smart_Fluo-Sensors|Selecting the Smart Fluo-Sensor]]'''« and »'''[[O2k-Fluo_LED2-Module#Setup_of_the_O2k-Fluo_LED2-Module|Setup of the O2k-Fluo LED2-Module]]'''«
|}
:::* Comments by Caro:
::::* For the question, do we use the term Fluorescence-Sensor or Smart Fluo-Sensors or fluorescence sensors which may include both Smart Fluo-Sensors (O2k-Fluo Smart-Module, https://www.oroboros.at/index.php/product/o2k-fluo-smart-module/) and Fluorescence-Sensors (O2k-Fluo LED2-Module, https://www.oroboros.at/index.php/product/o2k-fluo-led2-module/).
::::* I modified in the former text the text displayed for filters, hydrogen peroxide, mitochondrial membrane potential and calcium.
::::* Filters or LED filters and photodiode filters?
::::* The link from the filters should go to which page? [[Filters]] or https://www.oroboros.at/index.php/product/o2k-fluo-smart-module/ or https://wiki.oroboros.at/index.php/Talk:MitoPedia:_Fluorometry#Selecting_a_Filter_Set?
::::* Do we include in the entry the O2k-Fluo Smart-Module and the O2k-Fluo LED2-Module?
::::* In the last links, the first one goes to the "Select the Fluorescence-Sensors" page, but the second one does not go to the selection of filters.
::::* The link to "Select the Fluorescence-Sensors" goes to the O2k-Fluo LED2-Module and not to the section "Select the Smart Fluo-Sensors"
::::* Do we want to mention only the O2k-Fluo_Smart-Module or also the O2k-Fluo LED2-Module?
:::* Suggestions:
:{|
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]
| '''Do you know how to select the proper fluorescence sensors and filter set?''' <br>
Using different fluorescence sensors (with the [[O2k-Fluo_Smart-Module|O2k-Fluo Smart-Module]] or with the [https://wiki.oroboros.at/index.php/O2k-Fluo_LED2-Module|O2k-Fluo LED2-Module]) and LED and photodiode filters, the [https://www.oroboros.at/index.php/product/o2k-fluo-smart-module/ O2k-Fluo Smart-Module] allows for measurement of [[Hydrogen peroxide|hydrogen peroxide]], [[ATP]], [[Mitochondrial membrane potential|mitochondrial membrane potential]], [[Calcium|calcium]], and numerous other applications open for O2k-user innovation.<br>
- »'''[[O2k-Fluo_Smart-Module#Select_the_Smart_Fluo-Sensors|Selecting the Smart Fluo-Sensor]]'''« and »'''[https://wiki.oroboros.at/index.php/Talk:MitoPedia:_Fluorometry#Selecting_a_Filter_Set| Selecting a Filter Set]'''«
|}
:{|
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]
| '''Do you know how to select the proper fluorescence sensors and filter set?''' <br>
Using different Smart Fluo-Sensors and LED and photodiode filters, the [https://www.oroboros.at/index.php/product/o2k-fluo-smart-module/ O2k-Fluo Smart-Module] allows for measurement of [[Hydrogen peroxide|hydrogen peroxide]], [[ATP]], [[Mitochondrial membrane potential|mitochondrial membrane potential]], [[Calcium|calcium]], and numerous other applications open for O2k-user innovation.<br>
- »'''[[O2k-Fluo_Smart-Module#Select_the_Smart_Fluo-Sensors|Selecting the Smart Fluo-Sensor]]'''« and »'''[https://wiki.oroboros.at/index.php/Talk:MitoPedia:_Fluorometry#Selecting_a_Filter_Set| Selecting a Filter Set]'''«
|}
::::Comments by Timi:
:::::Suggestion:
:{|
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]
| '''Do you know how to select the proper fluorescence sensors and filter set?''' <br>
Using different fluorescence sensors, LED and photodiode filters, the [https://www.oroboros.at/index.php/product/o2k-fluo-smart-module/ O2k-Fluo Smart-Module] (or [https://wiki.oroboros.at/index.php/O2k-Fluo_LED2-Module O2k-Fluo LED2-Module]) allows for measurement of [[Hydrogen peroxide|hydrogen peroxide]], [[ATP]], [[Mitochondrial membrane potential|mitochondrial membrane potential]], [[Calcium|calcium]], and numerous other applications open for O2k-user innovation.<br>
- »'''[[O2k-Fluo_Smart-Module#Select_the_Smart_Fluo-Sensors|Selecting the Smart Fluo-Sensor]]'''« and »'''[[O2k-Fluo_LED2-Module#Setup_of_the_O2k-Fluo_LED2-Module| Selecting a Filter Set]]'''«
|}
:::::Arguments:
::::::• It refers generally to both O2k-Fluo Smart-Modul and O2k-Fluo LED2-Modul, therefore, I would write fluorescence sensors.
::::::• The link for Selecting a Filter Set what you put is not appropriate because it goes to a discussion page what we have not published so far, therefore, I would stick to the links in the original alerts.
::::::• The MitoPedia:Fluorometry discussion page does not contain new information. It it summary of information of all fluo-related pages. Therefore, you can find the same information on an other page.
::::::• In the original link the link of Selecting a Filter Set goes to the table with the sensors and filters and not to the listed points.
:::* Comments by Caro:
::::* Yes, I agree that the content of one of the suggestions which I provided is more appropiated.
::::* The question of the entry refers to how to select the appropiate fluorescence sensors and filters. At the end, the first link goes to the section Select the Smart Fluo-Sensors (this is good). The second link does not go to a page which helps with the second question (Selecting a Filter Set), it goes to the O2k-Fluo LED2-Module setup (what about the users with the O2k-FluoRespirometer) and then you can click in another link which goes to a page where some information about the filters can be found inside of the mounting filter section (which is not selecting a filter), and it is not as complete as it is shown in the link [[Talk:MitoPedia:_Fluorometry#Selecting_a_Filter_Set]]. Therefore, if I am an user, I do not see the question of the entry fixed. Why the content of the selection of filters is not moved to a front page?
:::* Final version:
:{|
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]
| '''Do you know how to select the proper fluorescence sensors and filter set?''' <br>
Using different fluorescence sensors, LED and photodiode filters, the [https://www.oroboros.at/index.php/product/o2k-fluo-smart-module/ O2k-Fluo Smart-Module] (or [https://wiki.oroboros.at/index.php/O2k-Fluo_LED2-Module O2k-Fluo LED2-Module]) allows for measurement of [[Hydrogen peroxide|hydrogen peroxide]], [[ATP]], [[Mitochondrial membrane potential|mitochondrial membrane potential]], [[Calcium|calcium]], and numerous other applications open for O2k-user innovation.<br>
- »'''[[O2k-Fluo_Smart-Module#Select_the_Smart_Fluo-Sensors|Selecting the Smart Fluo-Sensor]]'''« and »'''[https://wiki.oroboros.at/index.php/O2k-Fluo_LED2-Module#Fluorophores Selecting a Filter Set]'''«
|}
:{|
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]
| '''Do you know the different ways to adjust oxygen concentration in the Oroboros O2k?''' <br>
- »'''[[Setting_the_oxygen_concentration |Setting the oxygen concentration]]'''«
|}
:{|
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]
| '''Did you know that some chemicals are incompatible with the Amplex UltraRed assay?''' <br>
- »'''[https://wiki.oroboros.at/index.php/Amplex_UltraRed#Substances_incompatible_with_the_Amplex_UltraRed_assay Incompatible substrates]'''«
|}
:::* Comments by Caro:
::::* Question in bold as the other entries.


== In preparation ==
== In preparation ==

Revision as of 11:04, 26 August 2020

Antunes Diana (talk) 08:58, 5 May 2020 (CEST) Format for new Agenda/Alerts entries:
 {|
 | logos
 | title in bold <br>
 - link
 |}
 

e.g.,

O2k-Open SupportWarning: loss of data Did you know that the proper use of TIP2k-Filter Papers may avoid an unstable O2 slope neg. signal during Instrumental background correction?

- »Instrumental background« and »TIP2k Filter Papers«

See the scheme:


Agenda/Alerts body text format

Bioblast alert 2020

Prep for circular done here: Talk:Bioblast alert 2020

MitoPedia alert

Next

O2k-Brief alert

Next

  • Sep-04 Fr
O2k-brief repository Measuring ATP-ADP exchange via adenine nucleotide translocase (ANT) using the fluorophore Magnesium Green (MgG)
from the O2k-Network »HU Budapest Chinopoulos C« 
  • Chinopoulos C, Kiss G, Kawamata H, Starkov AA (2014) Measurement of ADP-ATP exchange in relation to mitochondrial transmembrane potential and oxygen consumption. Methods Enzymol 542:333-48. »Bioblast link«

O2k-Publications alert

Next

  • Sep-01 Tu


O2k-Publications in the MiPMap Complex I dysfunction induced by N-methyl carbamate can be alleviated with a cell-permeable succinate prodrug
from the O2k-Network »US PA Philadelphia Kilbaugh T«, »US PA Philadelphia Jang DH« and »SE Lund Elmer E«
  • Janowska JI, Piel S, Saliba N, Kim CD, Jang DH, Karlsson M, Kilbaugh TJ, Ehinger JK (2020) Mitochondrial respiratory chain Complex I dysfunction induced by N-methyl carbamate ex vivo can be alleviated with a cell-permeable succinate prodrug carbamate toxicity and treatment. Toxicol In Vitro 65:104794. »Bioblast link«

O2k-Open Support alert

Next

  • While in Home Office: Gnaigere (talk) 14:03, 19 March 2020 (CET) https://wiki.oroboros.at/index.php/Talk:O2k-Open_Support_alert - Many labs will move to home office mode. In line with our special DL7.4 home office offer (see Agenda today), therefore, I suggest to post in our Agenda analysis-linked O2k-Open Support cases during this time, and collect real-time operation cases for future releases.

Removed for correction

O2k-Open SupportWarning: loss of data Do you perform the leaky chamber test after chamber assembly?

An unnoticed leak from the chamber may affect your data, therefore we recommend performing the Leaky chamber test - especially after chamber reassembly.
- »Leaky chamber test« and » https://intern.oroboros.at/index.php/Overnight_test_after_O2k-chamber_assembly«

  • Aug-17 Mo
O2k-Open SupportWarning: loss of data Have you observed an 'O2 slope neg.' > 4 pmol∙s−1∙mL−1 in the closed 2 mL chamber at air saturation without a biological sample?

This may be due to biological contamination of the medium or the O2k-Chamber.
For further details on how to clean the O2k-Chamber properly, see:
- » Cleaning of the O2k-Chamber«

O2k-Open Support alert circulars

In preparation

For discussion

Here we discuss suggestions for the Oroboros Ecosystem Agenda 2020 (if the specific alert section is yet to be attributed)
MitoPedia: SUIT Controlled stability of the MitoKit-CII based on HPLC and LCMS analysis: No degradation of MitoKit-CII compounds MitoKit-CII/Succinate-nv (NV118) and MitoKit-CII/Malonate-nv (NV161) for following storage conditions:
  • Proven -80 °C solid stability for more than 3 years (3 years 7 months)
  • Proven -20 °C solid stability for 1 year
  • Proven DMSO stability at room temperature for 1 week
  • Proven DMSO stability at -20 °C for 6 months (ongoing)

2021

Postponed events

FAT4BRAIN » FAT4BRAIN School Riga LV IOC147«
»APS2020 Chicago US«
O2k-Workshops San Diego CA US
Bioenergetics Vienna 2020.jpg Bioenergetics Vienna 2020
O2k-Workshops IOC Schroecken AT