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Template talk:Next oroboros ecosystem agenda

From Bioblast
Antunes Diana (talk) 08:58, 5 May 2020 (CEST) Format for new Agenda/Alerts entries:
 {|
 | logos
 | title in bold <br>
 - link
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e.g.,

O2k-Open SupportWarning: loss of data Did you know that the proper use of TIP2k-Filter Papers may avoid an unstable O2 slope neg. signal during Instrumental background correction?

- »Instrumental background« and »TIP2k Filter Papers«

See the scheme:


Agenda/Alerts body text format

Bioblast alert 2020

Prep for circular done here: Talk:Bioblast alert 2020

MitoPedia alert

Next

  • Aug-26 We
MitoPedia
Why measuring ROX is important?

Rox is not necessarily equivalent to non-mitochondrial reduction of O2.
- »Residual oxygen consumption« and »BEC: Mitochondrial physiology«

Suggestion 1 by Timi:
MitoPedia
Why using the term residual oxygen consumption (Rox) is more accurate than non-mitochondrial respiration?

Rox refers rather to mitochondrial non-ET-pathway than non-mitochondrial respiration, because Rox can be also detected in purified mitochondria after ET-pathway inhibition, and it can be higher in pce and living cells than in isolated mitochondria.
- »BEC: Mitochondrial physiology«

  • I would change the question or keep the question but have a different answer, because I think that answer does not tell you why meausurement of ROX is important.
  • I moved the link to ROX to the question, because it might not be obvious for everyone what ROX means. Do you think we can have just one link to the BEC?
  • Should be define here what ROX means or the link is enough?
Suggestion 2 by Timi:
MitoPedia
Why measuring residual oxygen consumption (Rox) is important?

Rox refers to mitochondrial non-ET pathway related respiration rather than non-mitochondrial respiration due to oxidative side reactions remaining after ET-pathway inhibition.
- »BEC: Mitochondrial physiology«

  • I am not sure that this is a real answer to this question.

O2k-Brief alert

Next

  • ###-## Tu
O2k-brief repository Measuring ATP-ADP exchange via adenine nucleotide translocase (ANT) using the fluorophore Magnesium Green (MgG)
from the O2k-Network »HU Budapest Chinopoulos C« 
  • Chinopoulos C, Kiss G, Kawamata H, Starkov AA (2014) Measurement of ADP-ATP exchange in relation to mitochondrial transmembrane potential and oxygen consumption. Methods Enzymol 542:333-48. »Bioblast link«

O2k-Publications alert

Next

  • Aug-25 Tu
O2k-Publications in the MiPMap Low-intensity exercise stimulates bioenergetics and increases fat oxidation in mitochondria of blood mononuclear cells from sedentary adults
from the O2k-Network »LV Riga Makrecka-Kuka M«
  • Liepinsh E, Makarova E, Plakane L, Konrade I, Liepins K, Videja M, Sevostjanovs E, Grinberga S, Makrecka-Kuka M, Dambrova M (2020) Low-intensity exercise stimulates bioenergetics and increases fat oxidation in mitochondria of blood mononuclear cells from sedentary adults. Physiol Rep 8:e14489. »Bioblast link«

O2k-Open Support alert

Next

  • While in Home Office: Gnaigere (talk) 14:03, 19 March 2020 (CET) https://wiki.oroboros.at/index.php/Talk:O2k-Open_Support_alert - Many labs will move to home office mode. In line with our special DL7.4 home office offer (see Agenda today), therefore, I suggest to post in our Agenda analysis-linked O2k-Open Support cases during this time, and collect real-time operation cases for future releases.

Removed for correction

O2k-Open SupportWarning: loss of data Do you perform the leaky chamber test after chamber assembly?

An unnoticed leak from the chamber may affect your data, therefore we recommend performing the Leaky chamber test - especially after chamber reassembly.
- »Leaky chamber test« and » https://intern.oroboros.at/index.php/Overnight_test_after_O2k-chamber_assembly«

O2k-Open Support alert circulars

O2k-Open Support alert circular 2020(07): in prep

MitoPedia
Do you know how to select the proper Fluorescence-Sensor and filter set?

Using different Fluorescence-Sensors and filters, the O2k-Fluo Smart-Module allows for measurement of hydrogen peroxide, ATP, mitochondrial membrane potential, calcium, and numerous other applications open for O2k-user innovation.
- »Selecting the Smart Fluo-Sensor« and »Setup of the O2k-Fluo LED2-Module«

  • Comments by Caro:
  • Suggestions:
MitoPedia
Do you know how to select the proper fluorescence sensors and filter set?

Using different fluorescence sensors (with the O2k-Fluo Smart-Module or with the LED2-Module) and LED and photodiode filters, the O2k-Fluo Smart-Module allows for measurement of hydrogen peroxide, ATP, mitochondrial membrane potential, calcium, and numerous other applications open for O2k-user innovation.
- »Selecting the Smart Fluo-Sensor« and »Selecting a Filter Set«


MitoPedia
Do you know how to select the proper fluorescence sensors and filter set?

Using different Smart Fluo-Sensors and LED and photodiode filters, the O2k-Fluo Smart-Module allows for measurement of hydrogen peroxide, ATP, mitochondrial membrane potential, calcium, and numerous other applications open for O2k-user innovation.
- »Selecting the Smart Fluo-Sensor« and »Selecting a Filter Set«

Comments by Timi:
Suggestion:
MitoPedia
Do you know how to select the proper fluorescence sensors and filter set?

Using different fluorescence sensors, LED and photodiode filters, the O2k-Fluo Smart-Module (or O2k-Fluo LED2-Module) allows for measurement of hydrogen peroxide, ATP, mitochondrial membrane potential, calcium, and numerous other applications open for O2k-user innovation.
- »Selecting the Smart Fluo-Sensor« and » Selecting a Filter Set«

Arguments:
• It refers generally to both O2k-Fluo Smart-Modul and O2k-Fluo LED2-Modul, therefore, I would write fluorescence sensors.
• The link for Selecting a Filter Set what you put is not appropriate because it goes to a discussion page what we have not published so far, therefore, I would stick to the links in the original alerts.
• The MitoPedia:Fluorometry discussion page does not contain new information. It it summary of information of all fluo-related pages. Therefore, you can find the same information on an other page.
• In the original link the link of Selecting a Filter Set goes to the table with the sensors and filters and not to the listed points.
  • Comments by Caro:
  • Yes, I agree that the content of one of the suggestions which I provided is more appropiated.
  • The question of the entry refers to how to select the appropiate fluorescence sensors and filters. At the end, the first link goes to the section Select the Smart Fluo-Sensors (this is good). The second link does not go to a page which helps with the second question (Selecting a Filter Set), it goes to the O2k-Fluo LED2-Module setup (what about the users with the O2k-FluoRespirometer) and then you can click in another link which goes to a page where some information about the filters can be found inside of the mounting filter section (which is not selecting a filter), and it is not as complete as it is shown in the link Talk:MitoPedia:_Fluorometry#Selecting_a_Filter_Set. Therefore, if I am an user, I do not see the question of the entry fixed. Why the content of the selection of filters is not moved to a front page?
  • Final version:
MitoPedia
Do you know how to select the proper fluorescence sensors and filter set?

Using different fluorescence sensors, LED and photodiode filters, the O2k-Fluo Smart-Module (or O2k-Fluo LED2-Module) allows for measurement of hydrogen peroxide, ATP, mitochondrial membrane potential, calcium, and numerous other applications open for O2k-user innovation.
- »Selecting the Smart Fluo-Sensor« and »Selecting a Filter Set«


MitoPedia
Do you know the different ways to adjust oxygen concentration in the Oroboros O2k?

- »Setting the oxygen concentration«

MitoPedia
Did you know that some chemicals are incompatible with the Amplex UltraRed assay?

- »Incompatible substrates«

  • Comments by Caro:
  • Question in bold as the other entries.

In preparation

For discussion

Here we discuss suggestions for the Oroboros Ecosystem Agenda 2020 (if the specific alert section is yet to be attributed)
MitoPedia: SUIT Controlled stability of the MitoKit-CII based on HPLC and LCMS analysis: No degradation of MitoKit-CII compounds MitoKit-CII/Succinate-nv (NV118) and MitoKit-CII/Malonate-nv (NV161) for following storage conditions:
  • Proven -80 °C solid stability for more than 3 years (3 years 7 months)
  • Proven -20 °C solid stability for 1 year
  • Proven DMSO stability at room temperature for 1 week
  • Proven DMSO stability at -20 °C for 6 months (ongoing)

2021

Postponed events

FAT4BRAIN » FAT4BRAIN School Riga LV IOC147«
»APS2020 Chicago US«
O2k-Workshops San Diego CA US