Difference between revisions of "Thomas 2011 Mitochondrion"
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{{Publication | {{Publication | ||
|title=Thomas RR, Khan SM, Portell FR, Smigrodzki RM, Bennett JP Jr (2011) Recombinant human mitochondrial transcription factor A stimulates mitochondrial biogenesis and ATP synthesis, improves motor function after MPTP, reduces oxidative stress and increases survival after endotoxin. Mitochondrion 11: 108- | |title=Thomas RR, Khan SM, Portell FR, Smigrodzki RM, Bennett JP Jr (2011) Recombinant human mitochondrial transcription factor A stimulates mitochondrial biogenesis and ATP synthesis, improves motor function after MPTP, reduces oxidative stress and increases survival after endotoxin. Mitochondrion 11:108-18. | ||
|info=[http://www.ncbi.nlm.nih.gov/pubmed/20727424 PMID:20727424 ] | |info=[http://www.ncbi.nlm.nih.gov/pubmed/20727424 PMID: 20727424 Open Access] | ||
|authors=Thomas RR, Khan SM, Portell FR, Smigrodzki RM, Bennett JP | |authors=Thomas RR, Khan SM, Portell FR, Smigrodzki RM, Bennett JP | ||
|year=2011 | |year=2011 | ||
|journal=Mitochondrion | |journal=Mitochondrion | ||
|abstract=Recombinant human mitochondrial transcription factor A protein (rhTFAM) was evaluated for its acute effects on cultured cells and chronic effects in mice. Fibroblasts incubated with rhTFAM acutely increased respiration in a chloramphenicol-sensitive manner. SH-SY5Y cells showed rhTFAM concentration-dependent reduction of methylpyridinium (MPP+)-induced oxidative stress and increases in lowered ATP levels and viability. Mice treated with weekly i.v. rhTFAM showed increased mitochondrial gene copy number, Complex I protein levels and ATP production rates; oxidative damage to proteins was decreased ~50%. rhTFAM treatment improved motor recovery rate after treatment with MPTP and dose-dependently improved survival in the lipopolysaccharide model of endotoxin sepsis. | |abstract=Recombinant human mitochondrial transcription factor A protein (rhTFAM) was evaluated for its acute effects on cultured cells and chronic effects in mice. Fibroblasts incubated with rhTFAM acutely increased respiration in a chloramphenicol-sensitive manner. SH-SY5Y cells showed rhTFAM concentration-dependent reduction of methylpyridinium (MPP+)-induced oxidative stress and increases in lowered ATP levels and viability. Mice treated with weekly i.v. rhTFAM showed increased mitochondrial gene copy number, Complex I protein levels and ATP production rates; oxidative damage to proteins was decreased ~50%. rhTFAM treatment improved motor recovery rate after treatment with MPTP and dose-dependently improved survival in the lipopolysaccharide model of endotoxin sepsis. | ||
|keywords= | |keywords=Recombinant TFAM, Mitochondrial biogenesis, ATP synthesis, MPTP, Sepsis | ||
|mipnetlab= | |mipnetlab=US VA Richmond Bennett JP, US VA Richmond Iyer S | ||
}} | }} | ||
{{Labeling | {{Labeling | ||
|injuries=Oxidative stress;RONS | |||
|organism=Human, Mouse | |||
|tissues=Heart, Skeletal muscle, Nervous system, Fibroblast | |||
|preparations=Homogenate, Isolated mitochondria | |||
|topics=ATP, ATP production | |||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
}} | }} | ||
Latest revision as of 15:36, 9 November 2016
| Thomas RR, Khan SM, Portell FR, Smigrodzki RM, Bennett JP Jr (2011) Recombinant human mitochondrial transcription factor A stimulates mitochondrial biogenesis and ATP synthesis, improves motor function after MPTP, reduces oxidative stress and increases survival after endotoxin. Mitochondrion 11:108-18. |
Thomas RR, Khan SM, Portell FR, Smigrodzki RM, Bennett JP (2011) Mitochondrion
Abstract: Recombinant human mitochondrial transcription factor A protein (rhTFAM) was evaluated for its acute effects on cultured cells and chronic effects in mice. Fibroblasts incubated with rhTFAM acutely increased respiration in a chloramphenicol-sensitive manner. SH-SY5Y cells showed rhTFAM concentration-dependent reduction of methylpyridinium (MPP+)-induced oxidative stress and increases in lowered ATP levels and viability. Mice treated with weekly i.v. rhTFAM showed increased mitochondrial gene copy number, Complex I protein levels and ATP production rates; oxidative damage to proteins was decreased ~50%. rhTFAM treatment improved motor recovery rate after treatment with MPTP and dose-dependently improved survival in the lipopolysaccharide model of endotoxin sepsis. • Keywords: Recombinant TFAM, Mitochondrial biogenesis, ATP synthesis, MPTP, Sepsis
• O2k-Network Lab: US VA Richmond Bennett JP, US VA Richmond Iyer S
Labels:
Stress:Oxidative stress;RONS Organism: Human, Mouse Tissue;cell: Heart, Skeletal muscle, Nervous system, Fibroblast Preparation: Homogenate, Isolated mitochondria
Regulation: ATP, ATP production
HRR: Oxygraph-2k
