Difference between revisions of "O2k-Virtual Support self-study material"
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[[File:MITOEAGLE-representation.jpg|right|180px|link=http://www.mitoglobal.org/index.php/COST Action MitoEAGLE|COST Action MitoEAGLE]] | [[File:MITOEAGLE-representation.jpg|right|180px|link=http://www.mitoglobal.org/index.php/COST Action MitoEAGLE|COST Action MitoEAGLE]] | ||
__TOC__ | __TOC__ | ||
== O2k-Basic == | == O2k-Basic == | ||
=== Part 1.1: OroboPOS service and O2k instrumental | === Part 1.1: OroboPOS service and O2k instrumental === | ||
:::* [[O2k-FluoRespirometer|O2k-Manual: O2k-FluoRespirometer]] | :::* [[O2k-FluoRespirometer|O2k-Manual: O2k-FluoRespirometer]] | ||
:::* [[MiPNet19.18B POS-service|O2k-Manual: POS Service]] | :::* [[MiPNet19.18B POS-service|O2k-Manual: POS Service]] | ||
Revision as of 16:39, 19 October 2020
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O2k-Virtual Support self-study material |
MitoPedia O2k and high-resolution respirometry:
O2k-Open Support
O2k-Basic
Part 1.1: OroboPOS service and O2k instrumental
- O2k-Manual: O2k-FluoRespirometer
- O2k-Manual: POS Service
- O2k-Videosupport: OroboPOS
- O2k-Videosupport: Cathode cleaning
- O2k-Videosupport: Anode cleaning
- O2k-Videosupport: Membrane mounting
- O2k-Videosupport: Start DatLab 7
- O2k-Videosupport: Insert OroboPOS
- O2k-Videosupport: Insert O2k Chamber
- O2k-Videosupport: O2k Chamber volume calibration
Part 1.2: DatLab
Part 1.3: O2calibration and instrumental background
- Gnaiger 2008 POS
- SOP: O2k-cleaning and ISS
- SOP: O2-calibration
- O2k-Videosupport: QC1: POS calibration*
- SOP: O2 background
Part 2.1: Biological samples and experimental design
Pathway control and coupling control of mitochondrial respiration:
Sample types,mitochondrial preparations and sample addition:
- Sample
- Mitochondrial preparations
- MiPNet17.03 Shredder vs Fibres
- O2k-Videosupport: Shredder tissue homogenate
- O2k-Videosupport: Fiber permeabilization
- Cell count and normalization in HRR
- O2k-Videosupport: Addition of biological sample to the respirometer chamber*
SUIT protocols, SUITbrowser, DL-Protocols:
Part 2.2: Biological experiment and data analysis
O2k-Advanced
O2k-Applications: Simultaneous determination of O2 and H2O2fluxes
- Amplex UltraRed
- O2k-Videosupport: Smart Fluo-Sensor assembly*
- O2k-Videosupport: Exchanging a filter set of the smart-fluo sensor*
- Calibration with H2O2
- H2O2flux analysis
- O2k-Videosupport: H2O2flux analysis with DatLab 7.4*
NextGen-O2k KOL Training
- O2k-Videosupport: SUIT-030_PB_ce_D069 demo experiment *
- O2k-Videosupport: SUIT-030_PB_ce_D070 demo experiment*
* exclusive materials and links will be sent upon registration
