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Difference between revisions of "O2k-Virtual Support self-study material"

From Bioblast
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:::* [[Run DL-Protocol/Set O2 limit|DL-Protocols]]
:::* [[Run DL-Protocol/Set O2 limit|DL-Protocols]]


=== Part 1.3: O2calibration and instrumental background  ===
=== Part 1.3: O<sub>2</sub>calibration and instrumental background  ===
:::* [[Gnaiger 2008 POS]]
:::* [[Gnaiger 2008 POS]]
:::* [[MiPNet19.03 O2k-cleaning and ISS|SOP: O2k-cleaning and ISS]]
:::* [[MiPNet19.03 O2k-cleaning and ISS|SOP: O2k-cleaning and ISS]]
:::* [[MiPNet06.03 POS-calibration-SOP|SOP: O2-calibration]]
:::* [[MiPNet06.03 POS-calibration-SOP|SOP: O<sub>2</sub>-calibration]]
:::* O2k-Videosupport: QC1: POS calibration*
:::* O2k-Videosupport: QC1: POS calibration*
:::* [[MiPNet14.06 Instrumental O2 background|SOP: O2 background]]
:::* [[MiPNet14.06 Instrumental O2 background|SOP: O<sub>2</sub> background]]


=== Part 2.1: Biological samples and experimental design ===
=== Part 2.1: Biological samples and experimental design ===
Line 52: Line 52:
=== Part 2.2: Biological experiment and data analysis ===  
=== Part 2.2: Biological experiment and data analysis ===  
:::* [[MiPNet24.06 Oxygen flux analysis - DatLab 7.4|Oxygen_flux_analysis]]
:::* [[MiPNet24.06 Oxygen flux analysis - DatLab 7.4|Oxygen_flux_analysis]]
:::* [https://www.youtube.com/watch?v=HRAGvmaK01I O2k-Videosupport: O2flux analysis with DatLab 7.4]
:::* [https://www.youtube.com/watch?v=HRAGvmaK01I O2k-Videosupport: O<sub>2</sub> flux analysis with DatLab 7.4]




== O2k-Advanced ==
== O2k-Advanced ==
''' O2k-Applications: Simultaneous  determination  of  O2 and H2O2fluxes'''  
''' O2k-Applications: Simultaneous  determination  of  O<sub>2</sub> and H<sub>2</sub>O<sub>2</sub> fluxes'''  
:::* [[Amplex UltraRed]]
:::* [[Amplex UltraRed]]
:::* O2k-Videosupport: Smart Fluo-Sensor assembly*
:::* O2k-Videosupport: Smart Fluo-Sensor assembly*
:::* O2k-Videosupport: Exchanging a filter set of the smart-fluo sensor*
:::* O2k-Videosupport: Exchanging a filter set of the smart-fluo sensor*
:::* [[Amplex_UltraRed#Calibration_with_H2O2|Calibration with H2O2]]
:::* [[Amplex_UltraRed#Calibration_with_H2O2|Calibration with H<sub>2</sub>O<sub>2</sub>]]
:::* [[MiPNet24.10 H2O2 flux analysis|H2O2flux analysis]]
:::* [[MiPNet24.10 H2O2 flux analysis|H´<sub>2</sub>O<sub>2</sub> flux analysis]]
:::* O2k-Videosupport: H2O2flux analysis with DatLab 7.4*
:::* O2k-Videosupport: H<sub>2</sub>O<sub>2</sub> flux analysis with DatLab 7.4*





Revision as of 16:26, 19 October 2020


                  


O2k-Open Support

O2k-Virtual Support self-study material



Template NextGen-O2k.jpg


MitoPedia O2k and high-resolution respirometry: O2k-Open Support 



COST Action MitoEAGLE

General information

The materials for self-study, with the names and links to materials, are recommended to be studied before the respective training sessions of the Virtual O2k-Workshop.

O2k-Basic

Part 1.1: OroboPOS service and O2k instrumental

Part 1.2: DatLab

Part 1.3: O2calibration and instrumental background

Part 2.1: Biological samples and experimental design

Pathway control and coupling control of mitochondrial respiration:

Sample types,mitochondrial preparations and sample addition:

SUIT protocols, SUITbrowser, DL-Protocols:

Part 2.2: Biological experiment and data analysis


O2k-Advanced

O2k-Applications: Simultaneous determination of O2 and H2O2 fluxes


NextGen-O2k KOL Training

  • O2k-Videosupport: SUIT-030_PB_ce_D069 demo experiment *
  • O2k-Videosupport: SUIT-030_PB_ce_D070 demo experiment*
* exclusive materials and links will be sent upon registration