Difference between revisions of "O2k-Virtual Support self-study material"
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== General information == | == General information == | ||
:::: | :::: To benefit optimally from the tutored training sessions of the Virtual O2k-Workshop, please make yourself familiar with '''Materials for self-study''' in advance. These materials are either Open Access (OA) or will be sent upon registration (R). | ||
== O2k-Basic == | == O2k-Basic == | ||
=== Part 1.1: OroboPOS service and O2k instrumental === | === Part 1.1: OroboPOS service and O2k instrumental === | ||
:::* [[O2k-FluoRespirometer|O2k-Manual: O2k-FluoRespirometer]] | :::* [[O2k-FluoRespirometer|O2k-Manual: O2k-FluoRespirometer]] (OA) | ||
:::* [[MiPNet19.18B POS-service|O2k-Manual: POS Service]] | :::* [[MiPNet19.18B POS-service|O2k-Manual: POS Service]] (OA) | ||
:::* [https://www.youtube.com/watch?v=wMDcSypG3O4&feature=youtu.be O2k-Videosupport: OroboPOS] | :::* [https://www.youtube.com/watch?v=wMDcSypG3O4&feature=youtu.be O2k-Videosupport: OroboPOS] (OA) | ||
:::* [https://www.youtube.com/watch?v=HC6MLXO08k8&feature=youtu.be O2k-Videosupport: Cathode cleaning] | :::* [https://www.youtube.com/watch?v=HC6MLXO08k8&feature=youtu.be O2k-Videosupport: Cathode cleaning] (OA) | ||
:::* [https://www.youtube.com/watch?v=sDoU69-SLq0&feature=youtu.be O2k-Videosupport: Anode cleaning] | :::* [https://www.youtube.com/watch?v=sDoU69-SLq0&feature=youtu.be O2k-Videosupport: Anode cleaning] (OA) | ||
:::* [https://www.youtube.com/watch?v=iGafaPa4OyA&feature=youtu.be O2k-Videosupport: Membrane mounting] | :::* [https://www.youtube.com/watch?v=iGafaPa4OyA&feature=youtu.be O2k-Videosupport: Membrane mounting] (OA) | ||
:::* [https://www.youtube.com/watch?v=0EfCReolzdk&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=6 O2k-Videosupport: Start DatLab 7] | :::* [https://www.youtube.com/watch?v=0EfCReolzdk&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=6 O2k-Videosupport: Start DatLab 7] (OA) | ||
:::* [https://www.youtube.com/watch?v=pGzb0KfYwTQ&feature=youtu.be O2k-Videosupport: Insert OroboPOS] | :::* [https://www.youtube.com/watch?v=pGzb0KfYwTQ&feature=youtu.be O2k-Videosupport: Insert OroboPOS] (OA) | ||
:::* [https://www.youtube.com/watch?v=1MSsI1ePMu8&feature=youtu.be O2k-Videosupport: Insert O2k Chamber] | :::* [https://www.youtube.com/watch?v=1MSsI1ePMu8&feature=youtu.be O2k-Videosupport: Insert O2k Chamber] (OA) | ||
:::* [https://www.youtube.com/watch?v=DuaEJc7FBjk&feature=youtu.be O2k-Videosupport: O2k Chamber volume calibration] | :::* [https://www.youtube.com/watch?v=DuaEJc7FBjk&feature=youtu.be O2k-Videosupport: O2k Chamber volume calibration] (OA) | ||
=== Part 1.2: DatLab === | === Part 1.2: DatLab === | ||
:::* [[MitoPedia: DatLab]] | :::* [[MitoPedia: DatLab]] (OA) | ||
:::* [[Run DL-Protocol/Set O2 limit|DL-Protocols]] | :::* [[Run DL-Protocol/Set O2 limit|DL-Protocols]] (OA) | ||
=== Part 1.3: O<sub>2</sub>calibration and instrumental background === | === Part 1.3: O<sub>2</sub>calibration and instrumental background === | ||
:::* [[Gnaiger 2008 POS]] | :::* [[Gnaiger 2008 POS]] (OA) | ||
:::* [[MiPNet19.03 O2k-cleaning and ISS|SOP: O2k-cleaning and ISS]] | :::* [[MiPNet19.03 O2k-cleaning and ISS|SOP: O2k-cleaning and ISS]] (OA) | ||
:::* [[MiPNet06.03 POS-calibration-SOP|SOP: O<sub>2</sub>-calibration]] | :::* [[MiPNet06.03 POS-calibration-SOP|SOP: O<sub>2</sub>-calibration]] (OA) | ||
:::* O2k-Videosupport: QC1: POS calibration | :::* O2k-Videosupport: QC1: POS calibration (R) | ||
:::* [[MiPNet14.06 Instrumental O2 background|SOP: O<sub>2</sub> background]] | :::* [[MiPNet14.06 Instrumental O2 background|SOP: O<sub>2</sub> background]] (OA) | ||
=== Part 2.1: Biological samples and experimental design === | === Part 2.1: Biological samples and experimental design === | ||
'''Pathway control and coupling control of mitochondrial respiration:''' | '''Pathway control and coupling control of mitochondrial respiration:''' | ||
:::* [[Gnaiger 2020 MitoPathways|Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis]] | :::* [[Gnaiger 2020 MitoPathways|Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis]] (OA) | ||
:::* [[BEC 2020.1 doi10.26124bec2020-0001.v1|BEC 2020.1 Mitochondrial Physiology]] | :::* [[BEC 2020.1 doi10.26124bec2020-0001.v1|BEC 2020.1 Mitochondrial Physiology]] (OA) | ||
'''Sample types,mitochondrial preparations and sample addition:''' | '''Sample types,mitochondrial preparations and sample addition:''' | ||
:::* [[Sample]] | :::* [[Sample]] (OA) | ||
:::* [[Mitochondrial preparations]] | :::* [[Mitochondrial preparations]] (OA) | ||
:::* [[MiPNet17.03 Shredder vs Fibres]] | :::* [[MiPNet17.03 Shredder vs Fibres]] (OA) | ||
:::* [https://www.youtube.com/watch?v=EjQGUr5M8v0&feature=youtu.be O2k-Videosupport: Shredder tissue homogenate] | :::* [https://www.youtube.com/watch?v=EjQGUr5M8v0&feature=youtu.be O2k-Videosupport: Shredder tissue homogenate] (OA) | ||
:::* [https://www.youtube.com/watch?v=iN38rOkiBUQ&feature=youtu.be O2k-Videosupport: Fiber permeabilization] | :::* [https://www.youtube.com/watch?v=iN38rOkiBUQ&feature=youtu.be O2k-Videosupport: Fiber permeabilization] (OA) | ||
:::* [[Cell count and normalization in HRR]] | :::* [[Cell count and normalization in HRR]] (OA) | ||
:::* O2k-Videosupport: Addition of biological sample to the respirometer chamber | :::* O2k-Videosupport: Addition of biological sample to the respirometer chamber (R) | ||
'''SUIT protocols, SUITbrowser, DL-Protocols:''' | '''SUIT protocols, SUITbrowser, DL-Protocols:''' | ||
:::* [[MitoPedia: SUIT]] | :::* [[MitoPedia: SUIT]] (OA) | ||
:::* [https://suitbrowser.oroboros.at/ Oroboros SUITbrowser] | :::* [https://suitbrowser.oroboros.at/ Oroboros SUITbrowser] (OA) | ||
:::* [https://www.youtube.com/watch?v=8T33sp9KkJk O2k-Videosupport: How to find a DL-Protocol (DLP)] | :::* [https://www.youtube.com/watch?v=8T33sp9KkJk O2k-Videosupport: How to find a DL-Protocol (DLP)] (OA) | ||
:::* [https://www.youtube.com/watch?v=Vd66dHx-MyI O2k-Videosupport: Export DL-Protocol User (*.DLPU)] | :::* [https://www.youtube.com/watch?v=Vd66dHx-MyI O2k-Videosupport: Export DL-Protocol User (*.DLPU)] (OA) | ||
=== Part 2.2: Biological experiment and data analysis === | === Part 2.2: Biological experiment and data analysis === | ||
:::* [[MiPNet24.06 Oxygen flux analysis - DatLab 7.4|Oxygen_flux_analysis]] | :::* [[MiPNet24.06 Oxygen flux analysis - DatLab 7.4|Oxygen_flux_analysis]] (OA) | ||
:::* [https://www.youtube.com/watch?v=HRAGvmaK01I O2k-Videosupport: O<sub>2</sub> flux analysis with DatLab 7.4] | :::* [https://www.youtube.com/watch?v=HRAGvmaK01I O2k-Videosupport: O<sub>2</sub> flux analysis with DatLab 7.4] (OA) | ||
== O2k-Advanced == | == O2k-Advanced == | ||
''' O2k-Applications: Simultaneous determination of O<sub>2</sub> and H<sub>2</sub>O<sub>2</sub> fluxes''' | ''' O2k-Applications: Simultaneous determination of O<sub>2</sub> and H<sub>2</sub>O<sub>2</sub> fluxes''' | ||
:::* [[Amplex UltraRed]] | :::* [[Amplex UltraRed]] (OA) | ||
:::* O2k-Videosupport: Smart Fluo-Sensor assembly | :::* O2k-Videosupport: Smart Fluo-Sensor assembly (R) | ||
:::* O2k-Videosupport: Exchanging a filter set of the smart-fluo sensor | :::* O2k-Videosupport: Exchanging a filter set of the smart-fluo sensor (R) | ||
:::* [[Amplex_UltraRed#Calibration_with_H2O2|Calibration with H<sub>2</sub>O<sub>2</sub>]] | :::* [[Amplex_UltraRed#Calibration_with_H2O2|Calibration with H<sub>2</sub>O<sub>2</sub>]] (OA) | ||
:::* [[MiPNet24.10 H2O2 flux analysis|H<sub>2</sub>O<sub>2</sub> flux analysis]] | :::* [[MiPNet24.10 H2O2 flux analysis|H<sub>2</sub>O<sub>2</sub> flux analysis]] (OA) | ||
:::* O2k-Videosupport: H<sub>2</sub>O<sub>2</sub> flux analysis with DatLab 7.4 | :::* O2k-Videosupport: H<sub>2</sub>O<sub>2</sub> flux analysis with DatLab 7.4 (R) | ||
== NextGen-O2k KOL Training == | == NextGen-O2k KOL Training == | ||
:::* O2k-Videosupport: SUIT-030_PB_ce_D069 demo experiment | :::* O2k-Videosupport: SUIT-030_PB_ce_D069 demo experiment (R) | ||
:::* O2k-Videosupport: SUIT-030_PB_ce_D070 demo experiment | :::* O2k-Videosupport: SUIT-030_PB_ce_D070 demo experiment (R) | ||
Revision as of 09:26, 21 October 2020
O2k-Virtual Support self-study material |
MitoPedia O2k and high-resolution respirometry:
O2k-Open Support
General information
- To benefit optimally from the tutored training sessions of the Virtual O2k-Workshop, please make yourself familiar with Materials for self-study in advance. These materials are either Open Access (OA) or will be sent upon registration (R).
O2k-Basic
Part 1.1: OroboPOS service and O2k instrumental
- O2k-Manual: O2k-FluoRespirometer (OA)
- O2k-Manual: POS Service (OA)
- O2k-Videosupport: OroboPOS (OA)
- O2k-Videosupport: Cathode cleaning (OA)
- O2k-Videosupport: Anode cleaning (OA)
- O2k-Videosupport: Membrane mounting (OA)
- O2k-Videosupport: Start DatLab 7 (OA)
- O2k-Videosupport: Insert OroboPOS (OA)
- O2k-Videosupport: Insert O2k Chamber (OA)
- O2k-Videosupport: O2k Chamber volume calibration (OA)
Part 1.2: DatLab
- MitoPedia: DatLab (OA)
- DL-Protocols (OA)
Part 1.3: O2calibration and instrumental background
- Gnaiger 2008 POS (OA)
- SOP: O2k-cleaning and ISS (OA)
- SOP: O2-calibration (OA)
- O2k-Videosupport: QC1: POS calibration (R)
- SOP: O2 background (OA)
Part 2.1: Biological samples and experimental design
Pathway control and coupling control of mitochondrial respiration:
Sample types,mitochondrial preparations and sample addition:
- Sample (OA)
- Mitochondrial preparations (OA)
- MiPNet17.03 Shredder vs Fibres (OA)
- O2k-Videosupport: Shredder tissue homogenate (OA)
- O2k-Videosupport: Fiber permeabilization (OA)
- Cell count and normalization in HRR (OA)
- O2k-Videosupport: Addition of biological sample to the respirometer chamber (R)
SUIT protocols, SUITbrowser, DL-Protocols:
Part 2.2: Biological experiment and data analysis
O2k-Advanced
O2k-Applications: Simultaneous determination of O2 and H2O2 fluxes
- Amplex UltraRed (OA)
- O2k-Videosupport: Smart Fluo-Sensor assembly (R)
- O2k-Videosupport: Exchanging a filter set of the smart-fluo sensor (R)
- Calibration with H2O2 (OA)
- H2O2 flux analysis (OA)
- O2k-Videosupport: H2O2 flux analysis with DatLab 7.4 (R)
NextGen-O2k KOL Training
- O2k-Videosupport: SUIT-030_PB_ce_D069 demo experiment (R)
- O2k-Videosupport: SUIT-030_PB_ce_D070 demo experiment (R)